SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP013391
Component protein type	TssD
T6SS ID (Type)	T6SS01033 (Type i3)
Strain	Serratia sp. FS14
Replicon	chromosome
Sequence	Protein sequence (175 a.a.); Nucleotide sequence (525 bp)
Reference	[1] Li P, Xu D, Ma T, et al. Crystal structures of the kinase domain of PpkA, a key regulatory component of T6SS, reveal a general inhibitory mechanism. Biochem J. 2018 Jul 17;475(13):2209-2224. doi: 10.1042/BCJ20180077. PMID: 29858276

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	L085_RS12880
Coordinate (Strand)	2834120..2834644 (+)
NCBI ID	WP_004935369.1
RefSeq	NZ_CP005927
Uniprot ID	A0A5C7DDD9_9GAMM, A0A7C8CI70_9ENTR, A0A7X2USK1_9GAMM, A0A1B3FE12_9GAMM, A0A0A5TBI5_SERMA
KEGG ID	ssur:ATE40_012360
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_HCP	PF05638.14	2.7e-38	10..149

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-174	MSTKALVDYFLKIEGVDGESPDQTYPGWIQLQAWQWAEENAGRWGFGSGGGSGKVEMKDFEFRMVSNKASPKLFLMCATGEHIPQAKLVCRKSGQGQQDFIAVTFSNCLVSSFKTVGNMPLNSTIGTEIDTVLPTDVIKLNFARIEVEYKEQNNDGSMGAVIKTGYDLKLNARI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9864	-	-

Protein sequence: 175 a.a.

						>T6CP013391 NZ_CP005927:2834120-2834644 [Serratia sp. FS14] [TssD]
MSTKALVDYFLKIEGVDGESPDQTYPGWIQLQAWQWAEENAGRWGFGSGGGSGKVEMKDFEFRMVSNKASPKLFLMCATG
EHIPQAKLVCRKSGQGQQDFIAVTFSNCLVSSFKTVGNMPLNSTIGTEIDTVLPTDVIKLNFARIEVEYKEQNNDGSMGA
VIKTGYDLKLNARI*					

Nucleotide sequence: 525 bp

						>T6CP013391 NZ_CP005927:2834120-2834644 [Serratia sp. FS14] [TssD]
ATGAGCACGAAAGCTTTAGTAGACTATTTTTTGAAAATTGAGGGTGTTGATGGTGAATCACCCGACCAAACCTACCCTGG
CTGGATTCAGCTGCAAGCCTGGCAATGGGCGGAAGAAAACGCCGGCCGTTGGGGGTTCGGGAGCGGCGGCGGTTCAGGCA
AAGTCGAGATGAAAGACTTTGAGTTTCGCATGGTGAGCAACAAGGCTTCGCCGAAACTGTTCCTGATGTGCGCCACCGGC
GAGCATATTCCTCAGGCAAAACTTGTTTGCCGTAAATCAGGCCAGGGTCAGCAAGACTTCATTGCCGTGACCTTCAGCAA
CTGCCTGGTTTCCTCATTCAAGACCGTGGGTAATATGCCGCTGAATTCCACCATCGGTACTGAAATCGATACCGTGCTGC
CAACCGACGTTATCAAGCTGAATTTCGCACGTATCGAAGTTGAGTACAAAGAGCAAAACAATGATGGCTCCATGGGTGCG
GTAATTAAGACGGGGTATGACCTCAAGCTTAATGCCCGTATCTGA					