SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP013388
Component protein type	TssA
T6SS ID (Type)	T6SS01033 (Type i3)
Strain	Serratia sp. FS14
Replicon	chromosome
Sequence	Protein sequence (322 a.a.); Nucleotide sequence (966 bp)
Reference	[1] Li P, Xu D, Ma T, et al. Crystal structures of the kinase domain of PpkA, a key regulatory component of T6SS, reveal a general inhibitory mechanism. Biochem J. 2018 Jul 17;475(13):2209-2224. doi: 10.1042/BCJ20180077. PMID: 29858276

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	L085_RS12865
Coordinate (Strand)	2830818..2831783 (+)
NCBI ID	WP_004935378.1
RefSeq	NZ_CP005927
Uniprot ID	A0A0P0QGC3_SERMA
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	1.4e-11	12..122

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-321	MNEVISTEVNGVEFDPRYSEIEFTLSEYDSQTDPLNAVDNARDINWHAIREQSAALLEQCFDLHVVLWFMRANLHIDGFSAFYQGIKDIDAKYAEEGATIFPQADMEPSSDSFHAAALGWLATSSCLHEIKNSKIFPDTPLTTDELLNMRLDEQEGKSLHFSEVVKVLGQADSYFAGRNLPSLKEQLALEIDALERVENYANLQAEDYRLDCRQVRDYLKQFSRQLASLEQQELPAERDDAEIDSPDADAPVAMPGKYIRSRQDVILLLDQVLDYFQNYEPSHPAPILIRRSQKMIGMDFATIVEELLPESLASLNQLSGK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9912	-	-

Protein sequence: 322 a.a.

						>T6CP013388 NZ_CP005927:2830818-2831783 [Serratia sp. FS14] [TssA]
MNEVISTEVNGVEFDPRYSEIEFTLSEYDSQTDPLNAVDNARDINWHAIREQSAALLEQCFDLHVVLWFMRANLHIDGFS
AFYQGIKDIDAKYAEEGATIFPQADMEPSSDSFHAAALGWLATSSCLHEIKNSKIFPDTPLTTDELLNMRLDEQEGKSLH
FSEVVKVLGQADSYFAGRNLPSLKEQLALEIDALERVENYANLQAEDYRLDCRQVRDYLKQFSRQLASLEQQELPAERDD
AEIDSPDADAPVAMPGKYIRSRQDVILLLDQVLDYFQNYEPSHPAPILIRRSQKMIGMDFATIVEELLPESLASLNQLSG
K*					

Nucleotide sequence: 966 bp

						>T6CP013388 NZ_CP005927:2830818-2831783 [Serratia sp. FS14] [TssA]
ATGAATGAAGTCATCTCAACGGAAGTGAATGGCGTCGAATTTGATCCACGATACAGTGAAATTGAATTCACGCTTTCGGA
ATATGACAGCCAAACCGATCCGCTTAATGCCGTAGACAATGCGCGTGATATTAATTGGCACGCCATTCGCGAGCAGTCTG
CCGCGTTGCTGGAACAGTGCTTCGATTTACATGTCGTGCTGTGGTTTATGCGCGCCAATCTTCATATCGACGGTTTTTCG
GCCTTCTACCAGGGCATTAAGGATATTGACGCCAAATATGCCGAGGAGGGCGCCACCATTTTCCCTCAGGCGGACATGGA
ACCCTCATCGGACAGCTTTCATGCGGCCGCATTAGGGTGGCTCGCCACATCGTCCTGTCTGCACGAAATCAAGAACAGCA
AGATTTTTCCCGACACGCCGTTAACCACCGACGAGCTGCTGAATATGCGGCTTGACGAGCAGGAAGGCAAAAGCCTGCAT
TTTTCAGAGGTGGTAAAGGTGCTGGGGCAAGCCGACAGTTATTTTGCCGGCAGAAATCTGCCTTCGCTGAAAGAGCAATT
GGCGCTGGAAATCGATGCGCTGGAGCGCGTCGAGAATTACGCCAATCTGCAGGCTGAAGATTATCGTTTGGACTGCCGGC
AGGTACGCGACTATTTGAAACAGTTCAGCCGCCAGCTGGCTTCACTGGAACAGCAGGAATTGCCGGCTGAACGGGATGAC
GCGGAGATTGACTCGCCGGATGCTGACGCGCCTGTCGCCATGCCCGGCAAATATATCCGTTCCCGGCAGGATGTCATCTT
GCTGTTGGATCAGGTTTTGGACTATTTCCAAAACTATGAGCCCAGCCATCCGGCCCCCATTCTTATTCGACGTTCACAAA
AAATGATCGGTATGGACTTCGCGACGATTGTTGAGGAGCTTTTGCCGGAATCTCTGGCCTCGTTAAATCAATTGTCCGGG
AAGTAA					