SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP013345
Component protein type	TssJ
T6SS ID (Type)	T6SS01029 (Type i5)
Strain	Vibrio fluvialis 85003
Replicon	chromosome
Sequence	Protein sequence (172 a.a.); Nucleotide sequence (516 bp)
Reference	[1] Huang Y, Du P, Zhao M, et al. Functional Characterization and Conditional Regulation of the Type VI Secretion System in Vibrio fluvialis. Front Microbiol. 2017 Mar 30;8:528. doi: 10.3389/fmicb.2017.00528. eCollection 2017. PMID: 28424671

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	7325..7840 (-)
NCBI ID	1185549770
GenBank	KY319184
Uniprot ID	A0A1W6EUJ1_VIBFL
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	3.7e-28	37..154

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-171	MSWLNRIRLAAISCCALVLSACSSSSSTTTFAPTLKVPLVVEASKDINVFDDEQARPVVVRVYQLQDAGNFQKADFISLYNADQSVLADSLVDLQTLAPVLPGELRNLTLDVQQQSRFIAVLVEFAHYETATAKAYMALVSDPEEYPVYIHITKQKVELTQPVSKSWWNVF

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9986	pos: 21-22. LSA-CS.	MSWLNRIRLAAISCCALVLSA

Protein sequence: 172 a.a.

						>T6CP013345 KY319184:c7840-7325 [Vibrio fluvialis] [TssJ]
MSWLNRIRLAAISCCALVLSACSSSSSTTTFAPTLKVPLVVEASKDINVFDDEQARPVVVRVYQLQDAGNFQKADFISLY
NADQSVLADSLVDLQTLAPVLPGELRNLTLDVQQQSRFIAVLVEFAHYETATAKAYMALVSDPEEYPVYIHITKQKVELT
QPVSKSWWNVF*					

Nucleotide sequence: 516 bp

						>T6CP013345 KY319184:c7840-7325 [Vibrio fluvialis] [TssJ]
ATGAGCTGGCTGAATAGGATTCGCCTTGCGGCAATCAGCTGCTGCGCGTTGGTGCTGAGTGCTTGTAGTTCAAGCAGTTC
AACCACCACGTTTGCCCCCACACTCAAAGTGCCGCTGGTGGTCGAAGCGAGTAAAGACATCAATGTGTTTGATGATGAAC
AAGCGCGACCGGTGGTCGTACGCGTGTATCAGCTGCAGGATGCCGGCAATTTCCAGAAAGCCGATTTCATCAGCCTGTAC
AACGCGGATCAGAGCGTGCTGGCCGATTCGCTGGTGGATTTGCAGACACTCGCGCCTGTCCTGCCGGGAGAGCTGCGCAA
CCTGACCTTGGATGTCCAGCAACAAAGCCGCTTTATCGCCGTATTGGTCGAATTTGCACATTACGAAACCGCGACCGCCA
AAGCGTATATGGCGCTGGTGAGCGATCCCGAAGAGTACCCAGTTTATATCCACATCACCAAGCAGAAGGTTGAGCTGACT
CAACCGGTCAGCAAGTCGTGGTGGAACGTGTTTTAA					