Detailed information of component protein
Summary
| Component ID | T6CP013343 |
| Component protein type | TssM |
| T6SS ID (Type) | T6SS01029 |
| Strain | Vibrio fluvialis 85003 |
| Replicon | chromosome |
| Sequence | Protein sequence (1137 a.a.); Nucleotide sequence (3411 bp) |
| Reference |
External database links
| Locus tag (Gene) | - |
| Coordinate (Strand) | 1341..4751 (-) |
| NCBI ID | 1185549767 |
| GenBank | KY319184 |
| Uniprot ID | A0A1W6EUJ3_VIBFL |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| IcmF-related_N | PF14331.8 | 4.6e-52 | 165..416 |
| IcmF-related | PF06761.14 | 3.1e-35 | 472..765 |
| IcmF_C | PF06744.14 | 1.8e-15 | 1012..1117 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-6 | MMDKKK |
| Transmembrane helix | 7-29 | AILFSTLATFLVGLITCLSLRLT |
| Outside | 30-33 | GTYW |
| Transmembrane helix | 34-56 | LTPWWAVALQVTLIVLFLALVGW |
| Inside | 57-412 | GTYAYLRRPKVDETLKQHDEWRKRVLKNRFALIWKKHSKVTSNPYHVPWYVHVSADVSSDVRWLQQMGFESVESIEPVPDDLIAVRFWVSDSAVLVSVDMSFPLATRQASLDVVCELLMKKRDRQAFNGVLCTVDLDHLLQKNEIAASEFSQQYRTLLSDLNQRTGLSLPTYCVFTNMAAVRDFCELFSTLDEAAREQPFGVLRPADEGKGYDKDWFDESFDDLTRRLAITASESLKQQLHADFRASSVAGIFQLSALRYDIEDFLSLTFSQHAFDEVALHFRGFFFVNVGGEGESTDILTYMHASDLGFESLAGMQQHASSPSLFGKNLFRSCIVKEAPLVGVYRQREWRYRASR |
| Transmembrane helix | 413-430 | VAIFGGLLLLFGGFLWML |
| Outside | 431-1136 | KASFDYQQSLDNQALIKLNQYKENLKLDTIVADDLSSPVFSLSELRDINNLYNKQHRPWYVSDWLPDSSIRRFVHQTYYEELSSVLLTLMRDYILKDLFVYNSLDDKVKMLELLNYHQILYSKHRDDVDSLVNYYLTSLKEEGEGDVALLERFKLLAEDLFRSGATPPESEPELLALVRKSLSANDISELLYQHILQHPDFSRRVDMRTKLNPSYQNVFEFKPGFTGYLIPYLFTREGFEDLYNETGFQLASEAIKSYEGVMGNISGDAEMNRINRQLRERYIGDYVKYWQDLMANISWVEVSGWGEISKQIDMTVDPVYSPLSQFYNLVAVHTNLSQKKEGEPAPKESREAKEQQTEEGEPSVTAISNTSERVRASIAAPFKAMHQLVAVNEAGQSRLSIALAQLQQTRDWIEKSKETQDRGSYFLAQLQNTDSNNPLAQLNTLAADYNDPVLPVLMRGVARTTNRLAQSAMKTVINNDWSVLYGGYVRHFAGRYPFDKKAEFDASLADFSDFFKSGGQFDEFARKYAAYFDLTHGRQTQLKGFIVNQMLPLSDSYQPFSDAVRRMQSGLFIDGRVGVEFLLRAEKMSPDLTGFTLESGSKLFEYRNGPLLWQSLSWPIPANQTQDIAIITKDDKGAETREKLSGEWSWFRVADKMQSASVVGSDDVAWRYQSDSNEVSLKIKSKSAEQPFAPRFFDGLNIPIEL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.8745 | - | - |
Protein sequence: 1137 a.a.
>T6CP013343 KY319184:c4751-1341 [Vibrio fluvialis] [TssM]
MMDKKKAILFSTLATFLVGLITCLSLRLTGTYWLTPWWAVALQVTLIVLFLALVGWGTYAYLRRPKVDETLKQHDEWRKR
VLKNRFALIWKKHSKVTSNPYHVPWYVHVSADVSSDVRWLQQMGFESVESIEPVPDDLIAVRFWVSDSAVLVSVDMSFPL
ATRQASLDVVCELLMKKRDRQAFNGVLCTVDLDHLLQKNEIAASEFSQQYRTLLSDLNQRTGLSLPTYCVFTNMAAVRDF
CELFSTLDEAAREQPFGVLRPADEGKGYDKDWFDESFDDLTRRLAITASESLKQQLHADFRASSVAGIFQLSALRYDIED
FLSLTFSQHAFDEVALHFRGFFFVNVGGEGESTDILTYMHASDLGFESLAGMQQHASSPSLFGKNLFRSCIVKEAPLVGV
YRQREWRYRASRVAIFGGLLLLFGGFLWMLKASFDYQQSLDNQALIKLNQYKENLKLDTIVADDLSSPVFSLSELRDINN
LYNKQHRPWYVSDWLPDSSIRRFVHQTYYEELSSVLLTLMRDYILKDLFVYNSLDDKVKMLELLNYHQILYSKHRDDVDS
LVNYYLTSLKEEGEGDVALLERFKLLAEDLFRSGATPPESEPELLALVRKSLSANDISELLYQHILQHPDFSRRVDMRTK
LNPSYQNVFEFKPGFTGYLIPYLFTREGFEDLYNETGFQLASEAIKSYEGVMGNISGDAEMNRINRQLRERYIGDYVKYW
QDLMANISWVEVSGWGEISKQIDMTVDPVYSPLSQFYNLVAVHTNLSQKKEGEPAPKESREAKEQQTEEGEPSVTAISNT
SERVRASIAAPFKAMHQLVAVNEAGQSRLSIALAQLQQTRDWIEKSKETQDRGSYFLAQLQNTDSNNPLAQLNTLAADYN
DPVLPVLMRGVARTTNRLAQSAMKTVINNDWSVLYGGYVRHFAGRYPFDKKAEFDASLADFSDFFKSGGQFDEFARKYAA
YFDLTHGRQTQLKGFIVNQMLPLSDSYQPFSDAVRRMQSGLFIDGRVGVEFLLRAEKMSPDLTGFTLESGSKLFEYRNGP
LLWQSLSWPIPANQTQDIAIITKDDKGAETREKLSGEWSWFRVADKMQSASVVGSDDVAWRYQSDSNEVSLKIKSKSAEQ
PFAPRFFDGLNIPIEL*
MMDKKKAILFSTLATFLVGLITCLSLRLTGTYWLTPWWAVALQVTLIVLFLALVGWGTYAYLRRPKVDETLKQHDEWRKR
VLKNRFALIWKKHSKVTSNPYHVPWYVHVSADVSSDVRWLQQMGFESVESIEPVPDDLIAVRFWVSDSAVLVSVDMSFPL
ATRQASLDVVCELLMKKRDRQAFNGVLCTVDLDHLLQKNEIAASEFSQQYRTLLSDLNQRTGLSLPTYCVFTNMAAVRDF
CELFSTLDEAAREQPFGVLRPADEGKGYDKDWFDESFDDLTRRLAITASESLKQQLHADFRASSVAGIFQLSALRYDIED
FLSLTFSQHAFDEVALHFRGFFFVNVGGEGESTDILTYMHASDLGFESLAGMQQHASSPSLFGKNLFRSCIVKEAPLVGV
YRQREWRYRASRVAIFGGLLLLFGGFLWMLKASFDYQQSLDNQALIKLNQYKENLKLDTIVADDLSSPVFSLSELRDINN
LYNKQHRPWYVSDWLPDSSIRRFVHQTYYEELSSVLLTLMRDYILKDLFVYNSLDDKVKMLELLNYHQILYSKHRDDVDS
LVNYYLTSLKEEGEGDVALLERFKLLAEDLFRSGATPPESEPELLALVRKSLSANDISELLYQHILQHPDFSRRVDMRTK
LNPSYQNVFEFKPGFTGYLIPYLFTREGFEDLYNETGFQLASEAIKSYEGVMGNISGDAEMNRINRQLRERYIGDYVKYW
QDLMANISWVEVSGWGEISKQIDMTVDPVYSPLSQFYNLVAVHTNLSQKKEGEPAPKESREAKEQQTEEGEPSVTAISNT
SERVRASIAAPFKAMHQLVAVNEAGQSRLSIALAQLQQTRDWIEKSKETQDRGSYFLAQLQNTDSNNPLAQLNTLAADYN
DPVLPVLMRGVARTTNRLAQSAMKTVINNDWSVLYGGYVRHFAGRYPFDKKAEFDASLADFSDFFKSGGQFDEFARKYAA
YFDLTHGRQTQLKGFIVNQMLPLSDSYQPFSDAVRRMQSGLFIDGRVGVEFLLRAEKMSPDLTGFTLESGSKLFEYRNGP
LLWQSLSWPIPANQTQDIAIITKDDKGAETREKLSGEWSWFRVADKMQSASVVGSDDVAWRYQSDSNEVSLKIKSKSAEQ
PFAPRFFDGLNIPIEL*
Nucleotide sequence: 3411 bp
>T6CP013343 KY319184:c4751-1341 [Vibrio fluvialis] [TssM]
ATGATGGATAAGAAAAAAGCCATTCTCTTTTCAACGCTGGCGACATTCCTGGTTGGACTGATTACGTGTCTCTCGTTGAG
GCTGACCGGGACTTACTGGCTGACGCCGTGGTGGGCGGTGGCTCTGCAAGTGACGTTGATTGTGCTGTTTCTGGCGCTGG
TGGGATGGGGAACGTACGCGTACTTGCGTCGCCCGAAAGTGGATGAAACCCTCAAGCAGCACGACGAGTGGCGTAAACGC
GTGCTCAAGAACCGCTTTGCTCTGATTTGGAAAAAGCACAGTAAGGTGACCTCTAACCCGTATCATGTGCCTTGGTATGT
GCATGTGTCGGCGGACGTGAGTTCTGACGTTCGCTGGTTGCAACAGATGGGCTTTGAGAGTGTGGAATCCATAGAACCTG
TACCGGATGACTTGATTGCGGTGCGTTTTTGGGTCTCCGACAGTGCGGTGTTGGTGTCAGTTGATATGAGTTTCCCGCTC
GCTACCCGTCAGGCAAGCCTGGATGTGGTATGCGAACTGCTGATGAAAAAGCGCGATCGTCAGGCGTTCAATGGTGTTCT
GTGCACAGTCGATCTCGATCATCTGCTACAGAAAAACGAAATTGCGGCCAGCGAGTTCAGCCAGCAGTACCGAACGCTGC
TGAGCGATTTGAACCAACGTACCGGATTGTCGCTACCGACCTACTGCGTGTTTACCAATATGGCCGCAGTACGTGATTTC
TGTGAGTTGTTTTCGACGCTGGATGAAGCCGCACGTGAACAGCCTTTTGGTGTTCTGCGTCCGGCGGACGAAGGCAAAGG
CTATGATAAAGACTGGTTTGATGAATCGTTTGACGATCTGACCCGTCGCCTGGCGATCACGGCCAGCGAATCACTCAAAC
AACAGTTACACGCGGATTTTCGTGCCTCTTCGGTGGCGGGCATTTTCCAGCTCTCTGCTCTGCGCTACGACATTGAAGAC
TTTTTGTCGCTCACCTTCAGCCAGCATGCGTTTGATGAAGTTGCGCTGCATTTTCGCGGCTTCTTCTTCGTTAACGTCGG
CGGAGAAGGGGAATCCACTGACATTCTGACTTACATGCACGCCTCGGATCTCGGCTTTGAATCTCTGGCCGGTATGCAGC
AACACGCCTCCAGCCCGAGCCTGTTTGGCAAGAATCTGTTTCGCAGCTGTATTGTTAAAGAAGCGCCATTGGTGGGTGTA
TACCGTCAACGTGAGTGGCGTTACCGTGCTTCGCGAGTTGCAATTTTTGGTGGCTTATTGCTGCTGTTTGGTGGCTTTTT
GTGGATGTTAAAGGCCAGTTTTGACTATCAGCAGTCCTTGGACAATCAGGCGTTGATCAAACTGAATCAATACAAAGAGA
ATCTGAAACTGGATACGATTGTCGCCGATGATCTCTCATCGCCCGTGTTCAGTTTGTCGGAACTGCGAGACATCAACAAT
CTGTACAACAAACAGCATCGTCCTTGGTATGTCAGTGACTGGCTGCCGGATTCGAGTATCAGACGCTTCGTCCACCAAAC
GTATTATGAAGAGCTGAGCAGCGTACTGCTGACTCTGATGCGCGACTACATCCTCAAAGATCTGTTTGTCTACAACTCGC
TGGATGACAAAGTGAAAATGCTGGAGCTGCTGAACTATCATCAGATCCTGTATAGCAAACATCGTGATGATGTCGATTCG
TTGGTCAATTACTACCTGACTTCGCTCAAAGAGGAAGGGGAAGGCGATGTCGCACTGCTCGAACGCTTTAAGTTGCTGGC
CGAAGATCTGTTCCGCAGTGGGGCCACGCCGCCGGAATCGGAACCTGAACTGCTGGCGCTGGTGCGTAAGTCGCTGTCTG
CCAACGACATCAGTGAACTGCTCTATCAGCACATTTTGCAGCACCCGGATTTTTCACGCCGTGTGGATATGCGCACCAAG
CTGAATCCGTCCTATCAGAACGTGTTCGAGTTCAAGCCTGGCTTTACCGGCTATCTGATTCCGTATCTGTTTACCCGTGA
AGGCTTTGAAGATCTGTACAACGAAACCGGCTTTCAACTGGCGTCAGAAGCGATCAAATCTTATGAAGGTGTGATGGGCA
ATATCAGCGGTGATGCTGAAATGAACCGCATCAACCGCCAGTTGCGTGAACGCTATATCGGCGATTACGTCAAATACTGG
CAGGATTTGATGGCTAACATCAGCTGGGTGGAAGTGTCTGGATGGGGTGAGATCAGCAAACAGATCGATATGACGGTCGA
TCCGGTCTATTCGCCGCTCAGCCAGTTCTACAATCTGGTCGCGGTGCATACCAACCTGAGCCAGAAGAAAGAAGGCGAAC
CCGCGCCAAAAGAGAGCCGTGAAGCCAAAGAGCAACAGACGGAAGAGGGCGAACCGAGCGTGACCGCGATCAGCAATACC
AGCGAACGCGTGCGTGCATCGATTGCTGCGCCGTTCAAGGCGATGCATCAACTGGTGGCGGTGAACGAAGCGGGGCAAAG
CCGACTCAGTATTGCGCTGGCACAGTTGCAACAAACCCGTGACTGGATAGAGAAGTCCAAAGAAACGCAGGATCGCGGCA
GCTACTTCCTGGCGCAGTTGCAAAACACCGATTCCAATAACCCGTTGGCCCAGCTCAATACACTGGCGGCGGATTACAAT
GATCCCGTTCTGCCGGTATTGATGCGCGGCGTGGCGCGAACGACTAACCGATTGGCACAAAGTGCGATGAAAACGGTGAT
CAATAACGACTGGAGCGTGCTGTACGGCGGCTATGTGCGTCATTTTGCCGGCCGTTACCCGTTTGATAAGAAAGCAGAGT
TCGATGCCTCGTTGGCCGATTTTTCGGATTTCTTTAAATCCGGTGGTCAGTTTGATGAGTTTGCGCGGAAATACGCTGCC
TACTTTGACCTGACGCATGGGCGCCAAACGCAGCTCAAAGGCTTTATCGTCAATCAGATGCTGCCGTTGTCCGACTCTTA
TCAACCGTTCAGCGATGCGGTCAGACGCATGCAAAGTGGCCTGTTTATTGATGGGCGTGTCGGCGTCGAGTTTTTGCTGC
GGGCGGAAAAGATGTCGCCGGATCTGACCGGATTTACTCTCGAATCAGGCAGCAAACTGTTTGAATACCGCAATGGTCCG
CTGCTGTGGCAATCGCTCAGCTGGCCGATCCCGGCTAACCAAACTCAGGACATCGCCATCATCACCAAAGATGATAAAGG
GGCAGAAACCCGCGAGAAGCTGAGTGGTGAATGGAGCTGGTTCCGTGTCGCAGACAAGATGCAGAGCGCATCGGTGGTGG
GCAGTGATGATGTGGCCTGGCGCTATCAGTCAGACAGCAACGAAGTCAGCCTCAAGATTAAGTCGAAAAGTGCGGAGCAG
CCGTTTGCCCCCCGTTTCTTTGACGGCCTGAACATTCCAATCGAACTATGA
ATGATGGATAAGAAAAAAGCCATTCTCTTTTCAACGCTGGCGACATTCCTGGTTGGACTGATTACGTGTCTCTCGTTGAG
GCTGACCGGGACTTACTGGCTGACGCCGTGGTGGGCGGTGGCTCTGCAAGTGACGTTGATTGTGCTGTTTCTGGCGCTGG
TGGGATGGGGAACGTACGCGTACTTGCGTCGCCCGAAAGTGGATGAAACCCTCAAGCAGCACGACGAGTGGCGTAAACGC
GTGCTCAAGAACCGCTTTGCTCTGATTTGGAAAAAGCACAGTAAGGTGACCTCTAACCCGTATCATGTGCCTTGGTATGT
GCATGTGTCGGCGGACGTGAGTTCTGACGTTCGCTGGTTGCAACAGATGGGCTTTGAGAGTGTGGAATCCATAGAACCTG
TACCGGATGACTTGATTGCGGTGCGTTTTTGGGTCTCCGACAGTGCGGTGTTGGTGTCAGTTGATATGAGTTTCCCGCTC
GCTACCCGTCAGGCAAGCCTGGATGTGGTATGCGAACTGCTGATGAAAAAGCGCGATCGTCAGGCGTTCAATGGTGTTCT
GTGCACAGTCGATCTCGATCATCTGCTACAGAAAAACGAAATTGCGGCCAGCGAGTTCAGCCAGCAGTACCGAACGCTGC
TGAGCGATTTGAACCAACGTACCGGATTGTCGCTACCGACCTACTGCGTGTTTACCAATATGGCCGCAGTACGTGATTTC
TGTGAGTTGTTTTCGACGCTGGATGAAGCCGCACGTGAACAGCCTTTTGGTGTTCTGCGTCCGGCGGACGAAGGCAAAGG
CTATGATAAAGACTGGTTTGATGAATCGTTTGACGATCTGACCCGTCGCCTGGCGATCACGGCCAGCGAATCACTCAAAC
AACAGTTACACGCGGATTTTCGTGCCTCTTCGGTGGCGGGCATTTTCCAGCTCTCTGCTCTGCGCTACGACATTGAAGAC
TTTTTGTCGCTCACCTTCAGCCAGCATGCGTTTGATGAAGTTGCGCTGCATTTTCGCGGCTTCTTCTTCGTTAACGTCGG
CGGAGAAGGGGAATCCACTGACATTCTGACTTACATGCACGCCTCGGATCTCGGCTTTGAATCTCTGGCCGGTATGCAGC
AACACGCCTCCAGCCCGAGCCTGTTTGGCAAGAATCTGTTTCGCAGCTGTATTGTTAAAGAAGCGCCATTGGTGGGTGTA
TACCGTCAACGTGAGTGGCGTTACCGTGCTTCGCGAGTTGCAATTTTTGGTGGCTTATTGCTGCTGTTTGGTGGCTTTTT
GTGGATGTTAAAGGCCAGTTTTGACTATCAGCAGTCCTTGGACAATCAGGCGTTGATCAAACTGAATCAATACAAAGAGA
ATCTGAAACTGGATACGATTGTCGCCGATGATCTCTCATCGCCCGTGTTCAGTTTGTCGGAACTGCGAGACATCAACAAT
CTGTACAACAAACAGCATCGTCCTTGGTATGTCAGTGACTGGCTGCCGGATTCGAGTATCAGACGCTTCGTCCACCAAAC
GTATTATGAAGAGCTGAGCAGCGTACTGCTGACTCTGATGCGCGACTACATCCTCAAAGATCTGTTTGTCTACAACTCGC
TGGATGACAAAGTGAAAATGCTGGAGCTGCTGAACTATCATCAGATCCTGTATAGCAAACATCGTGATGATGTCGATTCG
TTGGTCAATTACTACCTGACTTCGCTCAAAGAGGAAGGGGAAGGCGATGTCGCACTGCTCGAACGCTTTAAGTTGCTGGC
CGAAGATCTGTTCCGCAGTGGGGCCACGCCGCCGGAATCGGAACCTGAACTGCTGGCGCTGGTGCGTAAGTCGCTGTCTG
CCAACGACATCAGTGAACTGCTCTATCAGCACATTTTGCAGCACCCGGATTTTTCACGCCGTGTGGATATGCGCACCAAG
CTGAATCCGTCCTATCAGAACGTGTTCGAGTTCAAGCCTGGCTTTACCGGCTATCTGATTCCGTATCTGTTTACCCGTGA
AGGCTTTGAAGATCTGTACAACGAAACCGGCTTTCAACTGGCGTCAGAAGCGATCAAATCTTATGAAGGTGTGATGGGCA
ATATCAGCGGTGATGCTGAAATGAACCGCATCAACCGCCAGTTGCGTGAACGCTATATCGGCGATTACGTCAAATACTGG
CAGGATTTGATGGCTAACATCAGCTGGGTGGAAGTGTCTGGATGGGGTGAGATCAGCAAACAGATCGATATGACGGTCGA
TCCGGTCTATTCGCCGCTCAGCCAGTTCTACAATCTGGTCGCGGTGCATACCAACCTGAGCCAGAAGAAAGAAGGCGAAC
CCGCGCCAAAAGAGAGCCGTGAAGCCAAAGAGCAACAGACGGAAGAGGGCGAACCGAGCGTGACCGCGATCAGCAATACC
AGCGAACGCGTGCGTGCATCGATTGCTGCGCCGTTCAAGGCGATGCATCAACTGGTGGCGGTGAACGAAGCGGGGCAAAG
CCGACTCAGTATTGCGCTGGCACAGTTGCAACAAACCCGTGACTGGATAGAGAAGTCCAAAGAAACGCAGGATCGCGGCA
GCTACTTCCTGGCGCAGTTGCAAAACACCGATTCCAATAACCCGTTGGCCCAGCTCAATACACTGGCGGCGGATTACAAT
GATCCCGTTCTGCCGGTATTGATGCGCGGCGTGGCGCGAACGACTAACCGATTGGCACAAAGTGCGATGAAAACGGTGAT
CAATAACGACTGGAGCGTGCTGTACGGCGGCTATGTGCGTCATTTTGCCGGCCGTTACCCGTTTGATAAGAAAGCAGAGT
TCGATGCCTCGTTGGCCGATTTTTCGGATTTCTTTAAATCCGGTGGTCAGTTTGATGAGTTTGCGCGGAAATACGCTGCC
TACTTTGACCTGACGCATGGGCGCCAAACGCAGCTCAAAGGCTTTATCGTCAATCAGATGCTGCCGTTGTCCGACTCTTA
TCAACCGTTCAGCGATGCGGTCAGACGCATGCAAAGTGGCCTGTTTATTGATGGGCGTGTCGGCGTCGAGTTTTTGCTGC
GGGCGGAAAAGATGTCGCCGGATCTGACCGGATTTACTCTCGAATCAGGCAGCAAACTGTTTGAATACCGCAATGGTCCG
CTGCTGTGGCAATCGCTCAGCTGGCCGATCCCGGCTAACCAAACTCAGGACATCGCCATCATCACCAAAGATGATAAAGG
GGCAGAAACCCGCGAGAAGCTGAGTGGTGAATGGAGCTGGTTCCGTGTCGCAGACAAGATGCAGAGCGCATCGGTGGTGG
GCAGTGATGATGTGGCCTGGCGCTATCAGTCAGACAGCAACGAAGTCAGCCTCAAGATTAAGTCGAAAAGTGCGGAGCAG
CCGTTTGCCCCCCGTTTCTTTGACGGCCTGAACATTCCAATCGAACTATGA