Detailed information of component protein


Summary

Component ID T6CP013342
Component protein type TssM
T6SS ID (Type) T6SS01028 experimental (Type i1)
Strain Vibrio fluvialis 85003
Replicon chromosome
Sequence Protein sequence (1182 a.a.); Nucleotide sequence (3546 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) -
Coordinate (Strand) 18151..21696 (+)
NCBI ID 1185549757
GenBank KY319183
Uniprot ID A0A0X8LJJ3_VIBFL
KEGG ID vfl:AL536_06680
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 2e-75 203..459
IcmF-related PF06761.14 1.1e-73 510..824
IcmF_C PF06744.14 1.2e-22 1054..1159
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-19 MWKFIVGIVRKLKPGMVAA
Transmembrane helix 20-42 LPILLFTTFILLNVAIWWAGPWL
Outside 43-51 EVSGHKPLE
Transmembrane helix 52-74 SVAARAVASSLFTLGAFAVWGFW
Inside 75-452 QWRKLQGYKAQQKRDEQLRQDPIQLFEERQEVELNQVMVNMKQNLNKRNYLYALPWYLVLGLENAGKTSLINRSGQKFVFSSVMRASGQKSENPYSFDWWIGDESVLIDPDGELLTQGNRSEDNDGAMERRLWLHFVNWLDRTRSRRPLNGIVLALDVSHLATATASERKAYASLLRARLRELMETLSTRLPVYIALTKLDLLYGFEPFFKHYTKSQREEVLGFTFSLDSIDNLDHWLEEFAKEYAEFVERINGVLPHAVSAPMSLDERNAIYSFTRQISGLKEILTQFFQDALASDQFSTSALVRGAYFTSVYQQGVPTNAFDDAASRRYGLSHAINTAQHAKNSTIYFTQKLFSNIIYPEAGLASDNFRIARQKRR
Transmembrane helix 453-472 LIALSFLTCSIATVLLAGTW
Outside 473-1181 HRNYLANIKHSDAVLAKVNQYKDQYPSNVYLASQKDVLEPLNKIREATLEFGFFREKPRFISDFGLYQGHTIGPMVEATYLNLLESRFLPLLMADVVVDLNQAQTDEEKLAVLRVYRMMVDKSGRYKDYVMDYFAKHWQQEFSGRREIQEELLGHLDYAMRHTDLAGERERGDKVANQVMKPYDKTIAKVQSDLGSMPNDQRVYRNLKLNAQTVLGPAINLRNLVGPVFDVVFEERVMNSSTLYIPQMLTKRGFEDYFMPQSESVSELALIDSWVLGQSKSAQFSEADKQALRDKIRNLYVADYTNTWRAALNEIDMKYFSDINDAVTVLDNLTGNIEPLQRLLRTLDDNTKMFAALPKDDSAQKELLKSPKYKVASMIEAPFSDLNNMIKPVGDKPAYLHEVLASVEELKNYLKSIQDAPDVGMAALDATKARVKLVNADPIYTLKRISSGLPKPLDTMVAKLADESWYVVKQEAIKHLEVRWHEDVYKTFEEKLAGRYPFNTASKKDASLEDFESFFAPNGTLDSFYNNQLRMFIEENIAVNNDDTSQSIIRKDVLSQIKQAQKIREAFFNRKGILDVSFSVEPLRLSGNKRRSVLNVDGQYLAYSHGPRDSVELIWPNTLRDSAVSKVTLVPTKANMSPRSINIQGPWAFFRLLDQGDVVAASTTSVDYKFAVDGGDMIYRINSEADANPFTERLFRSFKLSKTLY


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9966 - -


  Protein sequence: 1182 a.a.    

>T6CP013342 KY319183:18151-21696 [Vibrio fluvialis] [TssM]
MWKFIVGIVRKLKPGMVAALPILLFTTFILLNVAIWWAGPWLEVSGHKPLESVAARAVASSLFTLGAFAVWGFWQWRKLQ
GYKAQQKRDEQLRQDPIQLFEERQEVELNQVMVNMKQNLNKRNYLYALPWYLVLGLENAGKTSLINRSGQKFVFSSVMRA
SGQKSENPYSFDWWIGDESVLIDPDGELLTQGNRSEDNDGAMERRLWLHFVNWLDRTRSRRPLNGIVLALDVSHLATATA
SERKAYASLLRARLRELMETLSTRLPVYIALTKLDLLYGFEPFFKHYTKSQREEVLGFTFSLDSIDNLDHWLEEFAKEYA
EFVERINGVLPHAVSAPMSLDERNAIYSFTRQISGLKEILTQFFQDALASDQFSTSALVRGAYFTSVYQQGVPTNAFDDA
ASRRYGLSHAINTAQHAKNSTIYFTQKLFSNIIYPEAGLASDNFRIARQKRRLIALSFLTCSIATVLLAGTWHRNYLANI
KHSDAVLAKVNQYKDQYPSNVYLASQKDVLEPLNKIREATLEFGFFREKPRFISDFGLYQGHTIGPMVEATYLNLLESRF
LPLLMADVVVDLNQAQTDEEKLAVLRVYRMMVDKSGRYKDYVMDYFAKHWQQEFSGRREIQEELLGHLDYAMRHTDLAGE
RERGDKVANQVMKPYDKTIAKVQSDLGSMPNDQRVYRNLKLNAQTVLGPAINLRNLVGPVFDVVFEERVMNSSTLYIPQM
LTKRGFEDYFMPQSESVSELALIDSWVLGQSKSAQFSEADKQALRDKIRNLYVADYTNTWRAALNEIDMKYFSDINDAVT
VLDNLTGNIEPLQRLLRTLDDNTKMFAALPKDDSAQKELLKSPKYKVASMIEAPFSDLNNMIKPVGDKPAYLHEVLASVE
ELKNYLKSIQDAPDVGMAALDATKARVKLVNADPIYTLKRISSGLPKPLDTMVAKLADESWYVVKQEAIKHLEVRWHEDV
YKTFEEKLAGRYPFNTASKKDASLEDFESFFAPNGTLDSFYNNQLRMFIEENIAVNNDDTSQSIIRKDVLSQIKQAQKIR
EAFFNRKGILDVSFSVEPLRLSGNKRRSVLNVDGQYLAYSHGPRDSVELIWPNTLRDSAVSKVTLVPTKANMSPRSINIQ
GPWAFFRLLDQGDVVAASTTSVDYKFAVDGGDMIYRINSEADANPFTERLFRSFKLSKTLY*

  Nucleotide sequence: 3546 bp    

>T6CP013342 KY319183:18151-21696 [Vibrio fluvialis] [TssM]
ATGTGGAAATTTATTGTTGGAATAGTGAGAAAGCTTAAGCCAGGTATGGTGGCCGCTTTACCGATTTTGCTATTCACCAC
GTTCATTTTACTGAACGTCGCAATTTGGTGGGCGGGCCCTTGGCTAGAGGTTTCCGGTCACAAGCCGCTCGAGTCTGTGG
CTGCTCGCGCTGTGGCAAGCAGTTTGTTTACTCTGGGCGCATTCGCCGTTTGGGGCTTCTGGCAATGGCGTAAACTGCAA
GGCTATAAAGCGCAGCAGAAACGCGATGAGCAACTGCGCCAAGACCCGATTCAGCTGTTCGAAGAACGTCAGGAAGTCGA
ACTGAATCAGGTCATGGTCAACATGAAGCAGAACCTCAATAAGCGCAATTATTTGTATGCGCTACCGTGGTATCTGGTTT
TGGGGTTAGAAAATGCAGGCAAAACCAGCCTGATCAACCGCTCCGGCCAGAAATTCGTCTTCTCTTCAGTGATGCGTGCT
TCGGGACAGAAAAGCGAAAACCCGTACTCGTTCGACTGGTGGATCGGCGATGAGTCGGTACTGATTGACCCGGATGGTGA
GCTGCTGACTCAGGGCAATCGCAGTGAAGACAACGACGGTGCGATGGAGCGCCGTTTGTGGCTGCATTTTGTCAACTGGC
TGGACAGAACCCGCAGTCGCCGCCCGCTGAACGGTATCGTATTGGCGTTGGATGTGTCGCATTTGGCGACGGCGACGGCA
TCGGAGCGTAAAGCTTACGCCAGTCTGCTACGCGCTCGGCTGCGTGAACTGATGGAGACACTCTCAACGCGTTTACCAGT
GTATATTGCGCTGACCAAGCTTGACCTTCTGTACGGCTTTGAACCGTTTTTCAAACACTATACCAAGAGCCAGCGTGAAG
AAGTGCTGGGCTTTACTTTCTCGCTGGATTCAATTGATAACCTGGATCACTGGTTGGAAGAGTTTGCCAAGGAATACGCC
GAGTTTGTCGAGCGTATTAATGGGGTATTGCCACATGCGGTATCGGCACCAATGTCGCTGGATGAACGTAATGCCATCTA
CAGTTTTACTCGCCAGATCTCCGGTTTGAAAGAGATCCTGACGCAGTTTTTCCAAGATGCCTTGGCGAGTGATCAGTTCT
CAACCTCGGCACTGGTGCGCGGGGCTTACTTTACTTCGGTGTATCAGCAAGGTGTACCAACCAACGCATTCGATGATGCG
GCGTCTCGCCGCTACGGCCTGTCGCATGCGATCAATACTGCTCAGCACGCGAAAAACTCGACCATCTATTTCACCCAGAA
ACTGTTCAGTAACATCATTTACCCGGAAGCCGGACTGGCTTCGGATAACTTTCGGATTGCGCGCCAAAAGCGCCGCTTGA
TCGCGTTGTCGTTTCTCACTTGTTCAATTGCGACCGTTTTGCTTGCGGGGACCTGGCATCGCAACTATCTGGCTAACATC
AAGCATTCCGATGCGGTTCTGGCTAAGGTCAACCAGTACAAAGATCAGTATCCGTCCAATGTCTATCTGGCGTCGCAAAA
GGATGTGCTGGAGCCGCTGAACAAGATTCGTGAAGCCACGTTGGAATTTGGTTTCTTCCGTGAAAAACCCCGCTTTATTT
CTGACTTTGGTCTCTATCAGGGCCATACCATCGGGCCGATGGTGGAAGCGACGTATCTGAACCTGCTGGAAAGCCGCTTT
CTGCCTCTTTTGATGGCGGACGTGGTGGTCGATCTGAATCAGGCGCAGACGGATGAAGAGAAGCTGGCGGTGCTGAGGGT
GTACCGCATGATGGTGGATAAAAGCGGTCGCTATAAAGATTACGTGATGGATTACTTCGCGAAACATTGGCAGCAAGAGT
TCTCGGGCCGTCGTGAAATTCAGGAGGAACTGCTGGGTCACCTCGATTACGCCATGCGTCATACCGATCTGGCAGGCGAA
CGTGAACGCGGTGACAAAGTCGCGAATCAGGTGATGAAGCCTTATGATAAGACCATCGCGAAAGTGCAGAGCGATCTGGG
TTCTATGCCAAACGACCAACGCGTCTATCGTAACCTGAAGCTGAATGCGCAAACGGTGCTTGGCCCAGCGATTAACCTGC
GTAATCTGGTGGGGCCGGTATTTGATGTGGTGTTCGAAGAGCGCGTGATGAACAGCAGTACGCTGTATATTCCGCAGATG
CTCACCAAGCGCGGCTTTGAAGATTACTTTATGCCGCAGTCTGAATCGGTGTCGGAACTGGCATTGATTGACAGTTGGGT
ATTGGGACAGTCAAAGTCGGCGCAATTCAGTGAAGCGGACAAGCAAGCGCTGCGCGATAAGATTCGAAATCTGTATGTCG
CGGACTACACCAACACCTGGCGTGCGGCGTTAAATGAAATCGACATGAAATATTTCAGCGATATCAACGACGCCGTCACG
GTGTTAGACAACCTGACTGGCAACATTGAACCGCTGCAACGTCTGCTGCGCACACTCGACGACAACACCAAGATGTTTGC
CGCGCTGCCAAAAGACGATAGTGCTCAGAAAGAGCTGCTCAAGAGTCCCAAATATAAAGTGGCCTCCATGATCGAAGCGC
CGTTCTCTGATTTGAACAATATGATCAAACCGGTTGGAGACAAGCCTGCGTACCTCCATGAAGTGTTGGCATCGGTGGAA
GAGCTGAAGAACTATCTGAAATCCATTCAGGATGCGCCGGATGTTGGCATGGCAGCGCTTGATGCAACCAAGGCTCGAGT
GAAGCTCGTCAACGCGGACCCAATCTATACCTTGAAGCGCATCTCTTCTGGCTTGCCAAAACCGCTTGATACGATGGTCG
CCAAACTGGCGGATGAGAGCTGGTATGTAGTCAAGCAAGAGGCAATCAAACATCTTGAAGTACGTTGGCATGAAGATGTT
TATAAGACATTTGAAGAGAAACTGGCGGGTCGTTATCCGTTTAATACGGCATCGAAAAAAGATGCGTCACTGGAGGATTT
TGAATCCTTCTTTGCACCAAACGGCACGCTGGATAGCTTCTACAACAATCAGCTACGCATGTTCATTGAAGAGAACATCG
CTGTGAATAACGACGATACCTCACAGTCGATCATTCGCAAAGATGTACTGAGTCAGATAAAACAGGCGCAGAAAATCCGT
GAAGCCTTCTTTAACCGCAAAGGCATTCTGGATGTCAGTTTCTCGGTTGAGCCTCTGCGTCTGAGTGGCAACAAACGCCG
CAGTGTACTGAATGTCGACGGTCAATATCTGGCATACAGCCATGGCCCACGCGATAGCGTGGAGTTGATCTGGCCAAACA
CGTTGCGTGACTCGGCGGTATCGAAAGTGACTCTGGTTCCGACCAAAGCCAACATGTCACCACGCAGCATCAACATTCAG
GGACCATGGGCATTCTTCCGTCTGCTTGACCAGGGAGATGTGGTGGCTGCGAGCACCACGTCAGTGGACTACAAGTTCGC
GGTGGATGGCGGCGATATGATTTACCGCATCAACTCAGAAGCGGATGCCAACCCGTTCACAGAACGTTTGTTCAGATCGT
TCAAGCTATCTAAAACCTTGTATTAA