Detailed information of component protein
Summary
| Component ID | T6CP013339 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS01028  (Type i1) |
| Strain | Vibrio fluvialis 85003 |
| Replicon | chromosome |
| Sequence | Protein sequence (258 a.a.); Nucleotide sequence (774 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | - |
| Coordinate (Strand) | 11056..11829 (+) |
| NCBI ID | 1185549752 |
| GenBank | KY319183 |
| Uniprot ID | A0A109X140_VIBFL |
| KEGG ID | vfl:AL536_06705 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 7.8e-61 | 39..240 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-219 | MALSKKETQYSSLLFDDVQQINHDQDFWFQLRGDNPNVLIDAATPLFGLSLRVRSLAACDNIEQIYNQTIEEIKAIEIELAEQGFEHAILMAYRYILCAFLDEAVMGTEWGASSVWAEHSMLSRFHNETWGGEKVFTILSRLEGEPKRYQALLEFIYQCLVLGFEGKYRVMDGGQLEREKVIARLHGVLASLEEEEPQDLTRATDHVVRAKYKLSRQLP |
| Transmembrane helix | 220-242 | IWSIFAGFALLWVGLFLGYTYLL |
| Outside | 243-257 | HSKSSDVLNQLNQIL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9845 | - | - |
Protein sequence: 258 a.a.
>T6CP013339 KY319183:11056-11829 [Vibrio fluvialis] [TssL]
MALSKKETQYSSLLFDDVQQINHDQDFWFQLRGDNPNVLIDAATPLFGLSLRVRSLAACDNIEQIYNQTIEEIKAIEIEL
AEQGFEHAILMAYRYILCAFLDEAVMGTEWGASSVWAEHSMLSRFHNETWGGEKVFTILSRLEGEPKRYQALLEFIYQCL
VLGFEGKYRVMDGGQLEREKVIARLHGVLASLEEEEPQDLTRATDHVVRAKYKLSRQLPIWSIFAGFALLWVGLFLGYTY
LLHSKSSDVLNQLNQIL*
MALSKKETQYSSLLFDDVQQINHDQDFWFQLRGDNPNVLIDAATPLFGLSLRVRSLAACDNIEQIYNQTIEEIKAIEIEL
AEQGFEHAILMAYRYILCAFLDEAVMGTEWGASSVWAEHSMLSRFHNETWGGEKVFTILSRLEGEPKRYQALLEFIYQCL
VLGFEGKYRVMDGGQLEREKVIARLHGVLASLEEEEPQDLTRATDHVVRAKYKLSRQLPIWSIFAGFALLWVGLFLGYTY
LLHSKSSDVLNQLNQIL*
Nucleotide sequence: 774 bp
>T6CP013339 KY319183:11056-11829 [Vibrio fluvialis] [TssL]
ATGGCGTTAAGTAAAAAAGAGACCCAATACAGCAGCTTGCTCTTCGATGACGTGCAGCAGATCAACCATGATCAGGACTT
CTGGTTCCAACTGCGTGGCGACAATCCCAATGTGCTGATCGATGCGGCGACGCCGCTGTTTGGTTTGTCGCTGCGTGTCC
GTTCTTTGGCGGCCTGCGACAACATTGAGCAAATCTATAACCAGACCATTGAAGAGATCAAAGCTATCGAGATTGAGCTG
GCTGAACAAGGCTTTGAACACGCGATTCTGATGGCGTATCGCTACATCCTGTGCGCGTTTCTGGATGAAGCGGTGATGGG
CACCGAATGGGGCGCATCCAGTGTTTGGGCGGAGCATTCTATGCTGTCGCGTTTTCACAATGAAACGTGGGGCGGCGAGA
AAGTCTTCACTATTCTGAGCCGTTTGGAAGGCGAACCCAAGCGTTATCAGGCGCTGTTGGAATTCATTTATCAGTGCTTG
GTGTTGGGTTTTGAAGGCAAATACCGGGTGATGGACGGTGGTCAACTGGAACGTGAAAAGGTCATCGCCCGCTTACACGG
CGTTCTGGCGAGCCTTGAAGAGGAAGAGCCACAGGATCTCACGCGTGCCACTGATCATGTTGTGCGCGCCAAATATAAAC
TGAGCCGTCAGTTGCCAATCTGGTCGATTTTTGCCGGTTTTGCCCTGCTGTGGGTAGGACTCTTTCTCGGCTATACCTAT
TTGCTGCACAGCAAATCAAGCGACGTGTTAAATCAACTCAATCAAATACTTTAA
ATGGCGTTAAGTAAAAAAGAGACCCAATACAGCAGCTTGCTCTTCGATGACGTGCAGCAGATCAACCATGATCAGGACTT
CTGGTTCCAACTGCGTGGCGACAATCCCAATGTGCTGATCGATGCGGCGACGCCGCTGTTTGGTTTGTCGCTGCGTGTCC
GTTCTTTGGCGGCCTGCGACAACATTGAGCAAATCTATAACCAGACCATTGAAGAGATCAAAGCTATCGAGATTGAGCTG
GCTGAACAAGGCTTTGAACACGCGATTCTGATGGCGTATCGCTACATCCTGTGCGCGTTTCTGGATGAAGCGGTGATGGG
CACCGAATGGGGCGCATCCAGTGTTTGGGCGGAGCATTCTATGCTGTCGCGTTTTCACAATGAAACGTGGGGCGGCGAGA
AAGTCTTCACTATTCTGAGCCGTTTGGAAGGCGAACCCAAGCGTTATCAGGCGCTGTTGGAATTCATTTATCAGTGCTTG
GTGTTGGGTTTTGAAGGCAAATACCGGGTGATGGACGGTGGTCAACTGGAACGTGAAAAGGTCATCGCCCGCTTACACGG
CGTTCTGGCGAGCCTTGAAGAGGAAGAGCCACAGGATCTCACGCGTGCCACTGATCATGTTGTGCGCGCCAAATATAAAC
TGAGCCGTCAGTTGCCAATCTGGTCGATTTTTGCCGGTTTTGCCCTGCTGTGGGTAGGACTCTTTCTCGGCTATACCTAT
TTGCTGCACAGCAAATCAAGCGACGTGTTAAATCAACTCAATCAAATACTTTAA