Detailed information of component protein
Summary
| Component ID | T6CP013337 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS01028  (Type i1) |
| Strain | Vibrio fluvialis 85003 |
| Replicon | chromosome |
| Sequence | Protein sequence (159 a.a.); Nucleotide sequence (477 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | - |
| Coordinate (Strand) | 9236..9712 (+) |
| NCBI ID | 1185549750 |
| GenBank | KY319183 |
| Uniprot ID | A0A120L3G5_VIBFL |
| KEGG ID | vfl:AL536_06715 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 4.8e-29 | 30..144 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-158 | VNKLLFMLLSLLVLTACSSTEPYEPAKQPTKVTFSLLADDGVNPSIWGEASPVEIQVFELKDDSMFMSADYDQLKADYKKALRSNFVKNYDYVLMPDQFKFVNAFEVDEETNYIGVMAHFSDPEISEWKKAVKVLNKGREYHLLMFFKDYDVKLDRVE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9993 | pos: 16-17. LTA-CS. | VNKLLFMLLSLLVLTA |
Protein sequence: 159 a.a.
>T6CP013337 KY319183:9236-9712 [Vibrio fluvialis] [TssJ]
VNKLLFMLLSLLVLTACSSTEPYEPAKQPTKVTFSLLADDGVNPSIWGEASPVEIQVFELKDDSMFMSADYDQLKADYKK
ALRSNFVKNYDYVLMPDQFKFVNAFEVDEETNYIGVMAHFSDPEISEWKKAVKVLNKGREYHLLMFFKDYDVKLDRVE*
VNKLLFMLLSLLVLTACSSTEPYEPAKQPTKVTFSLLADDGVNPSIWGEASPVEIQVFELKDDSMFMSADYDQLKADYKK
ALRSNFVKNYDYVLMPDQFKFVNAFEVDEETNYIGVMAHFSDPEISEWKKAVKVLNKGREYHLLMFFKDYDVKLDRVE*
Nucleotide sequence: 477 bp
>T6CP013337 KY319183:9236-9712 [Vibrio fluvialis] [TssJ]
GTGAACAAACTGCTGTTTATGCTGCTTTCGTTGCTGGTATTGACCGCCTGCAGTTCGACGGAACCGTACGAGCCAGCGAA
ACAGCCGACGAAAGTCACGTTCAGTCTGCTCGCTGATGACGGGGTCAACCCTAGTATCTGGGGTGAAGCGTCTCCGGTCG
AGATTCAGGTGTTTGAACTCAAAGATGACTCGATGTTCATGTCCGCCGATTACGACCAACTGAAAGCGGATTACAAAAAG
GCGCTTCGCAGCAACTTCGTCAAAAATTACGACTACGTGCTGATGCCGGACCAGTTCAAGTTTGTGAATGCGTTTGAAGT
GGATGAAGAAACAAACTACATCGGCGTGATGGCGCACTTTTCTGATCCGGAAATCAGTGAATGGAAAAAGGCCGTCAAAG
TGCTGAACAAAGGCAGGGAATACCACCTGCTGATGTTTTTCAAAGATTACGATGTGAAATTGGATAGGGTGGAATAA
GTGAACAAACTGCTGTTTATGCTGCTTTCGTTGCTGGTATTGACCGCCTGCAGTTCGACGGAACCGTACGAGCCAGCGAA
ACAGCCGACGAAAGTCACGTTCAGTCTGCTCGCTGATGACGGGGTCAACCCTAGTATCTGGGGTGAAGCGTCTCCGGTCG
AGATTCAGGTGTTTGAACTCAAAGATGACTCGATGTTCATGTCCGCCGATTACGACCAACTGAAAGCGGATTACAAAAAG
GCGCTTCGCAGCAACTTCGTCAAAAATTACGACTACGTGCTGATGCCGGACCAGTTCAAGTTTGTGAATGCGTTTGAAGT
GGATGAAGAAACAAACTACATCGGCGTGATGGCGCACTTTTCTGATCCGGAAATCAGTGAATGGAAAAAGGCCGTCAAAG
TGCTGAACAAAGGCAGGGAATACCACCTGCTGATGTTTTTCAAAGATTACGATGTGAAATTGGATAGGGTGGAATAA