SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP013179
Component protein type	TssA
T6SS ID (Type)	T6SS01013 (Type i3)
Strain	Citrobacter rodentium DBS100
Replicon	chromosome
Sequence	Protein sequence (357 a.a.); Nucleotide sequence (1071 bp)
Reference	[1] Gueguen E, Wills NM, Atkins JF, et al. Transcriptional frameshifting rescues Citrobacter rodentium type VI secretion by the production of two length variants from the prematurely interrupted tssM gene. PLoS Genet. 2014 Dec 4;10(12):e1004869. doi: 10.1371/journal.pgen.1004869. eCollection 2014 Dec. PMID: 25474156

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	E2R62_10485
Coordinate (Strand)	2150532..2151602 (+)
NCBI ID	1609533876
GenBank	CP038008
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	2.1e-28	18..140

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-356	MNQTYPLTCSDYYQPLMSPISGSDACGETLDYDPAFIMLQSRLQPKLDAEYGNFIEAAEPINWAETERECQTLLQKSKDVRLIIILMRCRLRKVGLAALAEGLEALHALLCAWPDDLHPQLLDEGEFAPMLRANAFAELEDANGLLADLRNHPLPKASGLQITIKEFEKAHQLPREEGALSDTAMAAIIHEWQLNARDSLFPLTQAYGFLIEIKSVLAETLGHDAPEFAVLGNLLVYFANDFKGVSPSPVVLPSIVEDETPRVQEIAERPAGEVPAVLDLPPVAPPAVVEPRRAIDSRADALHRLQEVRCWFAMTEPSSPLIQVLKYAEESIGKSFADLLKMYPPEIVAMLNHEKE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9682	-	-

Protein sequence: 357 a.a.

						>T6CP013179 CP038008:2150532-2151602 [Citrobacter rodentium] [TssA]
MNQTYPLTCSDYYQPLMSPISGSDACGETLDYDPAFIMLQSRLQPKLDAEYGNFIEAAEPINWAETERECQTLLQKSKDV
RLIIILMRCRLRKVGLAALAEGLEALHALLCAWPDDLHPQLLDEGEFAPMLRANAFAELEDANGLLADLRNHPLPKASGL
QITIKEFEKAHQLPREEGALSDTAMAAIIHEWQLNARDSLFPLTQAYGFLIEIKSVLAETLGHDAPEFAVLGNLLVYFAN
DFKGVSPSPVVLPSIVEDETPRVQEIAERPAGEVPAVLDLPPVAPPAVVEPRRAIDSRADALHRLQEVRCWFAMTEPSSP
LIQVLKYAEESIGKSFADLLKMYPPEIVAMLNHEKE*					

Nucleotide sequence: 1071 bp

						>T6CP013179 CP038008:2150532-2151602 [Citrobacter rodentium] [TssA]
ATGAATCAAACATACCCTCTGACCTGTTCAGATTATTACCAGCCCCTGATGTCGCCCATCTCAGGCAGCGACGCCTGCGG
CGAGACGCTCGATTACGACCCCGCATTCATCATGTTACAGTCCCGGCTCCAGCCCAAACTGGACGCGGAATACGGCAACT
TCATTGAAGCGGCGGAGCCCATTAACTGGGCTGAAACCGAGCGCGAATGCCAGACCCTGCTGCAAAAAAGTAAAGACGTC
CGGCTGATTATCATCCTGATGCGCTGTCGGCTACGTAAAGTCGGCCTCGCCGCCCTGGCGGAAGGGCTGGAAGCGCTTCA
CGCCTTACTGTGTGCATGGCCGGACGATCTGCATCCGCAGCTTCTTGATGAAGGGGAGTTTGCTCCGATGCTGCGCGCCA
ACGCCTTCGCCGAGCTGGAGGATGCCAACGGCCTGCTTGCCGACCTGCGCAATCATCCGCTGCCGAAAGCCTCCGGCTTG
CAAATCACCATCAAAGAGTTTGAAAAAGCGCATCAGCTTCCGCGAGAAGAGGGCGCGCTGTCGGATACGGCCATGGCCGC
CATTATTCATGAATGGCAGCTTAACGCCCGTGACAGTCTCTTCCCGCTTACCCAGGCGTACGGCTTCCTGATTGAGATAA
AAAGCGTGCTTGCCGAAACGCTCGGGCACGATGCGCCAGAATTTGCCGTACTCGGTAACCTGCTGGTCTATTTCGCTAAT
GATTTCAAAGGCGTATCCCCTTCCCCGGTCGTTTTGCCGTCTATCGTGGAGGATGAAACGCCGCGGGTTCAGGAGATCGC
GGAACGTCCCGCCGGAGAGGTCCCCGCTGTGCTGGATCTTCCTCCCGTCGCGCCGCCTGCCGTCGTGGAACCGCGCCGCG
CTATCGACAGCCGCGCCGATGCGCTGCACCGCTTACAGGAAGTCCGCTGCTGGTTTGCGATGACCGAGCCCAGCAGCCCG
CTTATTCAGGTGCTGAAGTACGCGGAAGAGAGCATCGGCAAAAGCTTTGCCGACCTCTTAAAAATGTATCCCCCTGAGAT
CGTCGCCATGCTGAATCACGAGAAGGAATAA					