SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP013178
Component protein type	TssG
T6SS ID (Type)	T6SS01013 (Type i3)
Strain	Citrobacter rodentium DBS100
Replicon	chromosome
Sequence	Protein sequence (344 a.a.); Nucleotide sequence (1032 bp)
Reference	[1] Gueguen E, Wills NM, Atkins JF, et al. Transcriptional frameshifting rescues Citrobacter rodentium type VI secretion by the production of two length variants from the prematurely interrupted tssM gene. PLoS Genet. 2014 Dec 4;10(12):e1004869. doi: 10.1371/journal.pgen.1004869. eCollection 2014 Dec. PMID: 25474156

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	E2R62_10480
Coordinate (Strand)	2149504..2150535 (+)
NCBI ID	1609533875
GenBank	CP038008
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	1.3e-86	13..309

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-343	MKAVDAWFDDSKPWEAGFISTMRAIAARTPELPAPGKALLPSQERFRLGQVASMAFAPREIAAISQQDDKIKLQLFSLGIWGAQGAMPLHLSELAYSRYEQHDHTLIDFVDIFHHRALSLFYRAWFLSQDTASLDRQEDERFSFYVGSLIGLDPAELSPDPLPVHTRLASSAHLIREARNPDGLLGAMRYYFGVPVRMEEFELQWITLEHNDQTALGDSHCAGLLGNGAILGNTVLDRQHKFRLLMGPLTLAQYMLFSPWGSDMSVLRELVRSFIGFEYVWDVQLVLAADQVPQARLDGAHQLGYASWLERNACDDPVFGMSFEPEMYHRELTSVPTAKRYVR

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9540	-	-

Protein sequence: 344 a.a.

						>T6CP013178 CP038008:2149504-2150535 [Citrobacter rodentium] [TssG]
MKAVDAWFDDSKPWEAGFISTMRAIAARTPELPAPGKALLPSQERFRLGQVASMAFAPREIAAISQQDDKIKLQLFSLGI
WGAQGAMPLHLSELAYSRYEQHDHTLIDFVDIFHHRALSLFYRAWFLSQDTASLDRQEDERFSFYVGSLIGLDPAELSPD
PLPVHTRLASSAHLIREARNPDGLLGAMRYYFGVPVRMEEFELQWITLEHNDQTALGDSHCAGLLGNGAILGNTVLDRQH
KFRLLMGPLTLAQYMLFSPWGSDMSVLRELVRSFIGFEYVWDVQLVLAADQVPQARLDGAHQLGYASWLERNACDDPVFG
MSFEPEMYHRELTSVPTAKRYVR*					

Nucleotide sequence: 1032 bp

						>T6CP013178 CP038008:2149504-2150535 [Citrobacter rodentium] [TssG]
ATGAAAGCGGTTGACGCCTGGTTTGATGACAGCAAGCCCTGGGAAGCGGGTTTTATTAGCACAATGCGCGCGATTGCCGC
CCGCACTCCTGAGCTACCGGCCCCCGGCAAAGCCCTGCTGCCCTCGCAGGAGCGTTTTCGGCTGGGGCAAGTGGCCAGCA
TGGCCTTTGCGCCGCGTGAAATCGCCGCCATCAGCCAGCAGGATGACAAAATCAAGTTGCAGCTTTTTAGTTTGGGTATC
TGGGGGGCGCAGGGCGCCATGCCGCTTCACCTTTCCGAACTGGCTTACTCCCGCTACGAGCAGCACGACCATACGCTGAT
TGATTTTGTCGATATTTTTCACCACCGCGCGCTTAGCCTGTTTTATCGCGCGTGGTTTTTATCGCAGGATACCGCCTCGC
TGGACAGACAGGAGGACGAGCGGTTTTCGTTTTACGTCGGCAGCCTGATCGGCCTCGATCCAGCAGAACTCTCTCCCGAT
CCGTTACCTGTCCACACCCGGCTGGCCTCTTCCGCCCACCTTATTCGCGAAGCCCGTAACCCGGACGGCCTGCTGGGGGC
GATGCGCTACTACTTTGGCGTGCCGGTTCGCATGGAAGAGTTTGAACTGCAATGGATAACCCTTGAGCATAACGATCAAA
CCGCGCTCGGCGACAGCCATTGCGCCGGGCTACTGGGAAACGGCGCGATCCTCGGCAACACCGTGCTGGACAGACAACAT
AAATTCAGGCTGCTCATGGGACCGCTGACGCTTGCCCAGTACATGCTGTTCAGCCCGTGGGGCAGCGATATGTCAGTGCT
GCGTGAACTGGTGCGCAGTTTTATCGGTTTTGAATATGTCTGGGATGTGCAACTGGTACTGGCTGCCGATCAGGTGCCAC
AGGCCCGGCTTGATGGCGCCCACCAGCTTGGTTACGCAAGCTGGCTGGAGCGTAATGCTTGTGATGACCCGGTTTTCGGC
ATGAGCTTCGAGCCGGAAATGTATCACCGCGAGTTAACGTCTGTACCCACAGCAAAAAGATACGTGCGATGA					