SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP013175
Component protein type	TssB
T6SS ID (Type)	T6SS01013 (Type i3)
Strain	Citrobacter rodentium DBS100
Replicon	chromosome
Sequence	Protein sequence (173 a.a.); Nucleotide sequence (519 bp)
Reference	[1] Gueguen E, Wills NM, Atkins JF, et al. Transcriptional frameshifting rescues Citrobacter rodentium type VI secretion by the production of two length variants from the prematurely interrupted tssM gene. PLoS Genet. 2014 Dec 4;10(12):e1004869. doi: 10.1371/journal.pgen.1004869. eCollection 2014 Dec. PMID: 25474156

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	E2R62_10445
Coordinate (Strand)	2139387..2139905 (-)
NCBI ID	QBY29242.1
GenBank	CP038008
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	1.1e-58	11..161

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-172	MSKSNSQKFIARNRAPRVQIEYDVEIYGSEKKIELPFVMGVLADLSGKPLEPLPPVADRKFLSVDIDNFDERMKAMRPRVAFAVPNTLTGEGQLMVDMTFESMDDFSPDAIARKVDSLSQLLEARTQLANLQTYMDGKAGAESLVMKVLNDKSLLNTLASAPKPERADEVAS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9957	-	-

Protein sequence: 173 a.a.

						>T6CP013175 CP038008:c2139905-2139387 [Citrobacter rodentium] [TssB]
MSKSNSQKFIARNRAPRVQIEYDVEIYGSEKKIELPFVMGVLADLSGKPLEPLPPVADRKFLSVDIDNFDERMKAMRPRV
AFAVPNTLTGEGQLMVDMTFESMDDFSPDAIARKVDSLSQLLEARTQLANLQTYMDGKAGAESLVMKVLNDKSLLNTLAS
APKPERADEVAS*					

Nucleotide sequence: 519 bp

						>T6CP013175 CP038008:c2139905-2139387 [Citrobacter rodentium] [TssB]
ATGAGCAAGAGTAACTCACAAAAATTTATCGCACGTAATCGTGCGCCGCGCGTACAAATTGAGTACGACGTAGAAATCTA
TGGCAGTGAAAAGAAAATTGAATTGCCGTTCGTGATGGGCGTGCTGGCGGATTTATCCGGTAAACCGCTGGAGCCGCTGC
CGCCTGTGGCAGACCGCAAATTCCTGAGTGTCGATATTGATAACTTTGACGAGCGTATGAAAGCCATGCGTCCGCGTGTC
GCGTTCGCGGTGCCGAACACCTTAACGGGTGAAGGGCAACTGATGGTCGATATGACCTTCGAAAGCATGGATGATTTTTC
TCCGGATGCGATTGCCCGCAAAGTTGACTCGTTATCTCAGCTGCTTGAAGCCCGTACGCAGCTCGCCAATCTGCAGACCT
ATATGGACGGTAAAGCAGGCGCGGAGAGCCTGGTGATGAAGGTACTGAATGACAAGTCGCTGTTGAACACCCTGGCATCG
GCGCCAAAACCTGAACGGGCTGATGAAGTCGCGTCCTGA					