Detailed information of component protein
Summary
| Component ID | T6CP013172 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS01013  (Type i3) |
| Strain | Citrobacter rodentium DBS100 |
| Replicon | chromosome |
| Sequence | Protein sequence (187 a.a.); Nucleotide sequence (561 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | E2R62_10430 |
| Coordinate (Strand) | 2136603..2137163 (-) |
| NCBI ID | 1609533865 |
| GenBank | CP038008 |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 3.8e-20 | 49..156 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-186 | MASFGWIEWKRMLSGKEIKRVLKRTTVFLLSLFLVACSGSSRPASDGDAIKINLLAASDINPNEMGEPAPLNIFIYNVKDLDALTNADFYEIVEGTSKRVQAAASKVYEAILQPGESRTILIKPDGSTRSLAFIGAYRSLNYSLWLASWDLPVKKKSWWRPGFFSDDSLELNARFQKTAITIKVMD |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9939 | pos: 36-37. LVA-CS. | MASFGWIEWKRMLSGKEIKRVLKRTTVFLLSLFLVA |
Protein sequence: 187 a.a.
>T6CP013172 CP038008:c2137163-2136603 [Citrobacter rodentium] [TssJ]
MASFGWIEWKRMLSGKEIKRVLKRTTVFLLSLFLVACSGSSRPASDGDAIKINLLAASDINPNEMGEPAPLNIFIYNVKD
LDALTNADFYEIVEGTSKRVQAAASKVYEAILQPGESRTILIKPDGSTRSLAFIGAYRSLNYSLWLASWDLPVKKKSWWR
PGFFSDDSLELNARFQKTAITIKVMD*
MASFGWIEWKRMLSGKEIKRVLKRTTVFLLSLFLVACSGSSRPASDGDAIKINLLAASDINPNEMGEPAPLNIFIYNVKD
LDALTNADFYEIVEGTSKRVQAAASKVYEAILQPGESRTILIKPDGSTRSLAFIGAYRSLNYSLWLASWDLPVKKKSWWR
PGFFSDDSLELNARFQKTAITIKVMD*
Nucleotide sequence: 561 bp
>T6CP013172 CP038008:c2137163-2136603 [Citrobacter rodentium] [TssJ]
ATGGCGTCGTTCGGATGGATTGAGTGGAAACGTATGCTTAGCGGAAAAGAAATAAAACGCGTACTTAAGAGAACAACCGT
TTTTTTACTGTCACTCTTTTTAGTCGCCTGTAGTGGAAGCAGCCGTCCGGCGAGCGATGGCGATGCAATAAAAATAAATC
TGCTGGCGGCAAGCGATATTAACCCAAATGAAATGGGCGAACCGGCGCCACTCAATATATTTATTTATAACGTCAAGGAT
CTGGATGCTTTAACCAATGCGGACTTTTATGAAATTGTTGAGGGGACCAGTAAACGTGTACAGGCGGCAGCGTCAAAAGT
CTATGAAGCGATTTTACAACCCGGAGAGTCGCGCACCATTCTGATTAAACCAGACGGTTCTACACGCAGTCTTGCTTTCA
TTGGCGCCTATCGCAGCCTTAATTATTCTCTCTGGCTGGCGAGTTGGGATTTGCCCGTAAAGAAAAAATCGTGGTGGCGA
CCGGGTTTCTTTAGCGATGATTCGCTTGAGCTGAATGCGCGTTTCCAGAAAACAGCAATAACAATTAAAGTAATGGATTA
A
ATGGCGTCGTTCGGATGGATTGAGTGGAAACGTATGCTTAGCGGAAAAGAAATAAAACGCGTACTTAAGAGAACAACCGT
TTTTTTACTGTCACTCTTTTTAGTCGCCTGTAGTGGAAGCAGCCGTCCGGCGAGCGATGGCGATGCAATAAAAATAAATC
TGCTGGCGGCAAGCGATATTAACCCAAATGAAATGGGCGAACCGGCGCCACTCAATATATTTATTTATAACGTCAAGGAT
CTGGATGCTTTAACCAATGCGGACTTTTATGAAATTGTTGAGGGGACCAGTAAACGTGTACAGGCGGCAGCGTCAAAAGT
CTATGAAGCGATTTTACAACCCGGAGAGTCGCGCACCATTCTGATTAAACCAGACGGTTCTACACGCAGTCTTGCTTTCA
TTGGCGCCTATCGCAGCCTTAATTATTCTCTCTGGCTGGCGAGTTGGGATTTGCCCGTAAAGAAAAAATCGTGGTGGCGA
CCGGGTTTCTTTAGCGATGATTCGCTTGAGCTGAATGCGCGTTTCCAGAAAACAGCAATAACAATTAAAGTAATGGATTA
A