SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP013171
Component protein type	TssK
T6SS ID (Type)	T6SS01013 (Type i3)
Strain	Citrobacter rodentium DBS100
Replicon	chromosome
Sequence	Protein sequence (449 a.a.); Nucleotide sequence (1347 bp)
Reference	[1] Gueguen E, Wills NM, Atkins JF, et al. Transcriptional frameshifting rescues Citrobacter rodentium type VI secretion by the production of two length variants from the prematurely interrupted tssM gene. PLoS Genet. 2014 Dec 4;10(12):e1004869. doi: 10.1371/journal.pgen.1004869. eCollection 2014 Dec. PMID: 25474156

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	E2R62_10425
Coordinate (Strand)	2135253..2136599 (-)
NCBI ID	1609533864
GenBank	CP038008
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VasE	PF05936.14	3.3e-141	19..446

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-448	VRTNKVVWSEGLFLRPQLFQQQERYFEYYAHKRASTLSPFFWGFSQYEIDREALSYGKLVLRAARGVLPDGTPFDVPDHADLPEPLTILPEHLGKTIYLAVPLRLDNSDETIFSQHDVSSLARFRAQETELCDTNAIRQGPKPVQLAKLRLRLMSQTEMTESWIGLPLTRVKAIQPDGSVLLHLDDYIPPVTGYAANPLLSEWLTHLNGLVKMRAGMLAHRLSASDGKASASSEVADYLLLQIFNKYEPILDHLRHIPELPPIMLYEELAKLAGELSTFVRTKTRRPRSAPGYDHASLYPSIRPLVDDVHDLLNQILVRAGQLIDLQAKGNGVWAASMLPNELCSFSNLVLAVHAQLPMDVLLQQFQAQAKISAPQQLHELVRSHLPGLALQGLPVPPRQIPYSTGYVYFELLKKGPFWDKISGIGALALHIAGEFPGLKMELWGIRN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9859	-	-

Protein sequence: 449 a.a.

						>T6CP013171 CP038008:c2136599-2135253 [Citrobacter rodentium] [TssK]
VRTNKVVWSEGLFLRPQLFQQQERYFEYYAHKRASTLSPFFWGFSQYEIDREALSYGKLVLRAARGVLPDGTPFDVPDHA
DLPEPLTILPEHLGKTIYLAVPLRLDNSDETIFSQHDVSSLARFRAQETELCDTNAIRQGPKPVQLAKLRLRLMSQTEMT
ESWIGLPLTRVKAIQPDGSVLLHLDDYIPPVTGYAANPLLSEWLTHLNGLVKMRAGMLAHRLSASDGKASASSEVADYLL
LQIFNKYEPILDHLRHIPELPPIMLYEELAKLAGELSTFVRTKTRRPRSAPGYDHASLYPSIRPLVDDVHDLLNQILVRA
GQLIDLQAKGNGVWAASMLPNELCSFSNLVLAVHAQLPMDVLLQQFQAQAKISAPQQLHELVRSHLPGLALQGLPVPPRQ
IPYSTGYVYFELLKKGPFWDKISGIGALALHIAGEFPGLKMELWGIRN*					

Nucleotide sequence: 1347 bp

						>T6CP013171 CP038008:c2136599-2135253 [Citrobacter rodentium] [TssK]
GTGAGAACGAATAAAGTCGTCTGGAGTGAAGGGCTGTTTCTTCGTCCGCAGCTTTTTCAACAGCAAGAACGCTATTTTGA
ATACTACGCACATAAAAGAGCATCGACCCTGAGCCCCTTTTTTTGGGGATTCTCGCAGTATGAAATCGACCGCGAGGCGC
TGTCGTATGGCAAGCTGGTGCTGCGTGCCGCGCGCGGCGTATTGCCGGACGGTACGCCGTTTGATGTGCCCGATCACGCC
GATTTACCCGAGCCGCTAACGATTTTGCCGGAACATCTGGGCAAGACGATTTATCTGGCGGTACCGTTACGCCTTGATAA
TAGCGATGAAACTATCTTCAGCCAGCATGACGTCAGTTCGCTGGCGCGTTTTCGCGCGCAGGAGACGGAACTGTGCGACA
CCAACGCGATTCGCCAGGGGCCGAAGCCGGTTCAACTGGCCAAACTGCGGCTCAGGCTGATGTCGCAAACGGAAATGACC
GAATCGTGGATTGGCCTGCCGCTGACCCGCGTCAAGGCGATTCAGCCTGATGGCAGCGTGCTGTTGCATCTGGACGATTA
TATCCCGCCCGTCACCGGCTATGCGGCGAATCCGCTGCTGAGCGAATGGCTTACCCATCTTAATGGCCTGGTCAAAATGC
GTGCCGGGATGCTGGCGCACCGCCTGTCGGCGTCCGACGGCAAGGCCAGCGCCAGCTCGGAGGTGGCGGATTATCTGCTG
TTGCAGATTTTCAATAAATACGAGCCGATTCTCGATCACCTGCGCCATATTCCGGAACTCCCGCCTATCATGCTTTACGA
AGAGCTGGCGAAGCTGGCCGGTGAGCTTTCGACTTTTGTGCGCACCAAAACGCGTCGTCCCAGGAGCGCGCCGGGCTATG
ACCACGCCAGCCTTTATCCTTCCATTCGTCCCCTGGTCGACGATGTGCATGACCTGCTTAACCAGATCCTGGTGCGGGCC
GGGCAGCTTATTGACCTGCAGGCCAAAGGCAACGGCGTCTGGGCTGCCTCGATGCTGCCGAACGAACTGTGTTCGTTCTC
AAACCTGGTGCTGGCGGTTCATGCGCAGCTGCCGATGGATGTGTTGCTGCAGCAATTCCAGGCGCAGGCCAAAATCAGCG
CGCCGCAACAGCTCCATGAACTGGTTCGATCGCACCTGCCGGGCCTGGCGCTGCAAGGGCTGCCGGTACCGCCGCGTCAG
ATCCCGTACAGCACGGGTTACGTCTATTTTGAACTGCTCAAAAAGGGGCCTTTCTGGGACAAGATTTCAGGCATCGGCGC
GCTGGCGCTGCATATTGCGGGTGAGTTCCCTGGCCTGAAGATGGAACTTTGGGGAATCAGGAACTGA					