Detailed information of component protein


Summary

Component ID T6CP013103
Component protein type TssL
T6SS ID (Type) T6SS01295 experimental (Type i3)
Strain Burkholderia pseudomallei K96243
Replicon chromosome II
Sequence Protein sequence (553 a.a.); Nucleotide sequence (1659 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) AQ15_5516
Coordinate (Strand) 2520235..2521893 (+)
NCBI ID 772957888
GenBank CP009537
Uniprot ID Q63MW7_BURPS
KEGG ID bps:BPSS0531
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 2.9e-42 121..316
OmpA PF00691.22 2.8e-09 376..483
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-294 MLDRAAANPDLVTRVPTLSSLSSAAMTSSAVSTTGTTNTVASGGAAAGAPSFAPPAADAFPQADGAPRNPAVLQFPVPGGAPAPADARVAAPVVYSAQGEQAAIMKAGLQQASWNNPFVSHALPAVLQLQRHLAAGPLNQAAIRTQLGLEVRLYRERLAASGCEWEQIRDASYLLCTYLDETVNDAAREHAQVVYDGERSLLVEFHDDAWGGEDAFADLSRWMKTEPPPIPLLSFYELILSLGWQGRYRVLDRGDVLLQDLRSQLHALIWHHVPPEPLGTELVAPAKRRRSWWT
Transmembrane helix 295-317 AGRAAAVALGVLVLAYGAISFWL
Outside 318-552 DSQGRPIRNALAAWMPPTRTINIAETLPPPLPQILTEGWLTAYKHPQGWLLVFKSDGAFDVGKANVRADFMHNIERLGLAFAPWPGDLEVIGHTDSRPIRTSEFPDNQALSEARARNVADELRKTALPGGARAPENAVQRNIEYSGRGDAQPIDTAKTAAAYERNRRVDVLWKVIPDGAQQSGRSLNLQQPEKPGQVPMRPAMPEGVEIAPDGQLPYATSTTMPATRPTTEGRQP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
TAT(Tat/SPI) 0.5432 pos: 59-60. ADA-FP. MLDRAAANPDLVTRVPTLSSLSSAAMTSSAVSTTGTTNTVASGGAAAGAPSFAPPAADA


  Protein sequence: 553 a.a.    

>T6CP013103 CP009537:2520235-2521893 [Burkholderia pseudomallei K96243] [TssL]
MLDRAAANPDLVTRVPTLSSLSSAAMTSSAVSTTGTTNTVASGGAAAGAPSFAPPAADAFPQADGAPRNPAVLQFPVPGG
APAPADARVAAPVVYSAQGEQAAIMKAGLQQASWNNPFVSHALPAVLQLQRHLAAGPLNQAAIRTQLGLEVRLYRERLAA
SGCEWEQIRDASYLLCTYLDETVNDAAREHAQVVYDGERSLLVEFHDDAWGGEDAFADLSRWMKTEPPPIPLLSFYELIL
SLGWQGRYRVLDRGDVLLQDLRSQLHALIWHHVPPEPLGTELVAPAKRRRSWWTAGRAAAVALGVLVLAYGAISFWLDSQ
GRPIRNALAAWMPPTRTINIAETLPPPLPQILTEGWLTAYKHPQGWLLVFKSDGAFDVGKANVRADFMHNIERLGLAFAP
WPGDLEVIGHTDSRPIRTSEFPDNQALSEARARNVADELRKTALPGGARAPENAVQRNIEYSGRGDAQPIDTAKTAAAYE
RNRRVDVLWKVIPDGAQQSGRSLNLQQPEKPGQVPMRPAMPEGVEIAPDGQLPYATSTTMPATRPTTEGRQP*

  Nucleotide sequence: 1659 bp    

>T6CP013103 CP009537:2520235-2521893 [Burkholderia pseudomallei K96243] [TssL]
ATGCTCGATCGCGCGGCGGCGAATCCGGATCTCGTGACGCGCGTGCCGACGCTGTCGTCGCTGTCGAGCGCGGCGATGAC
GAGCTCCGCCGTATCGACCACCGGCACGACGAACACGGTGGCGTCGGGCGGCGCGGCCGCGGGCGCGCCGAGCTTCGCGC
CGCCGGCCGCCGACGCGTTTCCCCAAGCCGACGGCGCGCCCCGCAACCCCGCGGTGCTGCAATTCCCGGTGCCGGGCGGC
GCGCCCGCGCCCGCCGACGCGCGGGTGGCAGCGCCCGTCGTCTACAGCGCGCAGGGCGAGCAGGCCGCGATCATGAAAGC
AGGCCTGCAGCAGGCGAGCTGGAACAACCCGTTCGTGTCGCACGCGCTGCCCGCGGTGCTGCAACTGCAGCGCCACCTCG
CGGCCGGCCCGCTCAATCAGGCCGCGATCCGCACGCAGCTCGGCCTCGAGGTGCGGCTCTACCGCGAGCGGCTCGCCGCC
TCCGGCTGCGAATGGGAGCAGATCCGCGACGCGTCGTACCTGCTCTGCACGTATCTCGACGAAACCGTCAACGACGCGGC
GCGCGAGCACGCGCAAGTCGTCTACGACGGCGAGCGCAGCCTGCTCGTCGAATTCCACGACGACGCGTGGGGCGGCGAGG
ACGCGTTCGCCGACCTGTCGCGCTGGATGAAGACCGAGCCGCCGCCGATTCCGCTTCTGTCGTTCTACGAACTGATCCTG
TCGCTCGGCTGGCAGGGCCGCTACCGCGTGCTCGACCGCGGCGACGTGCTGCTGCAGGATCTGCGCTCGCAACTGCACGC
GCTGATCTGGCATCACGTGCCGCCCGAGCCGCTCGGCACCGAGCTCGTCGCGCCCGCGAAGCGGCGCCGCTCGTGGTGGA
CGGCCGGGCGCGCGGCGGCCGTCGCGCTCGGCGTGCTGGTGCTCGCGTACGGCGCGATCAGCTTCTGGCTCGATTCGCAG
GGCCGCCCGATCCGCAACGCGCTCGCCGCGTGGATGCCGCCCACGCGCACGATCAACATCGCCGAGACGCTGCCGCCGCC
GCTGCCGCAGATTCTCACCGAAGGGTGGCTCACCGCGTACAAGCATCCGCAAGGATGGCTGCTCGTGTTCAAGAGCGACG
GCGCGTTCGACGTCGGCAAGGCGAACGTGCGGGCGGACTTCATGCACAACATCGAGCGGCTCGGCCTCGCGTTCGCGCCG
TGGCCGGGCGACCTCGAGGTGATCGGCCACACCGATTCGCGGCCGATCCGCACGAGCGAGTTCCCGGACAACCAGGCGCT
GTCCGAAGCGCGGGCGCGCAACGTCGCCGACGAACTGCGCAAGACCGCGCTGCCGGGCGGCGCGCGCGCGCCGGAGAACG
CGGTGCAGCGCAACATCGAGTACTCGGGGCGCGGCGACGCGCAGCCGATCGACACCGCGAAGACGGCCGCCGCGTACGAG
CGCAACCGCCGCGTCGACGTGCTGTGGAAGGTGATTCCCGACGGCGCGCAGCAATCGGGCCGCAGCCTGAACCTGCAGCA
GCCGGAGAAGCCCGGGCAGGTGCCGATGCGTCCGGCGATGCCGGAGGGCGTGGAGATCGCGCCTGACGGGCAACTGCCGT
ATGCGACGTCAACCACGATGCCAGCAACGCGACCGACCACGGAGGGCCGTCAGCCATGA