Detailed information of component protein


Summary

Component ID T6CP013080
Component protein type TssL
T6SS ID (Type) T6SS01294 experimental (Type i3)
Strain Burkholderia pseudomallei K96243
Replicon chromosome II
Sequence Protein sequence (422 a.a.); Nucleotide sequence (1266 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) AQ15_5129
Coordinate (Strand) 2022451..2023716 (-)
NCBI ID 772957998
GenBank CP009537
Uniprot ID Q63NY0_BURPS
KEGG ID bps:BPSS0168
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 4.3e-72 47..254
OmpA PF00691.22 7e-16 315..413
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-233 VHHPDISAPAPTFDSVAATFARREPAPAPAGEPPAARLAAIRLARNPLLEAARVLLRALADMPERLDRDDIPQLRLLLEQEVRLFQRLCEQANIRRDHMLGARYCLCTALDEAAMQTSWAQSASGNLGTWISEGLATSFHEDRQGGDKVYLLIGRLMNSPHEHIDLLEVIYRILSLGFEGRYRYEADGQRKHETVRQRLYNEIASQRGPVSVALSPHWQPGPRSRSAPFRDFP
Transmembrane helix 234-256 AWVTAAVLSLIALGLFGCFKYAL
Inside 257-421 STRSADVQQRIAAIARMAPPAAPAELRLATLLAGEIAAGTLSVEENARRSSVTFRGDAMFAPGAAGVNPAMGPLIRKIAAEIAKVPGKVTVRGYTDNQPIKSRQFASNEALSEERATQVMQMLQSAGVPASRLEALGKGGAEPIGDNRTPQGRALNRRVEITVAR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9575 - -


  Protein sequence: 422 a.a.    

>T6CP013080 CP009537:c2023716-2022451 [Burkholderia pseudomallei K96243] [TssL]
VHHPDISAPAPTFDSVAATFARREPAPAPAGEPPAARLAAIRLARNPLLEAARVLLRALADMPERLDRDDIPQLRLLLEQ
EVRLFQRLCEQANIRRDHMLGARYCLCTALDEAAMQTSWAQSASGNLGTWISEGLATSFHEDRQGGDKVYLLIGRLMNSP
HEHIDLLEVIYRILSLGFEGRYRYEADGQRKHETVRQRLYNEIASQRGPVSVALSPHWQPGPRSRSAPFRDFPAWVTAAV
LSLIALGLFGCFKYALSTRSADVQQRIAAIARMAPPAAPAELRLATLLAGEIAAGTLSVEENARRSSVTFRGDAMFAPGA
AGVNPAMGPLIRKIAAEIAKVPGKVTVRGYTDNQPIKSRQFASNEALSEERATQVMQMLQSAGVPASRLEALGKGGAEPI
GDNRTPQGRALNRRVEITVAR*

  Nucleotide sequence: 1266 bp    

>T6CP013080 CP009537:c2023716-2022451 [Burkholderia pseudomallei K96243] [TssL]
GTGCATCATCCGGACATATCGGCTCCGGCCCCGACCTTCGATTCCGTCGCGGCGACGTTCGCGCGGCGCGAGCCGGCGCC
TGCGCCGGCCGGCGAGCCGCCCGCCGCGCGCCTCGCCGCGATCAGGCTCGCCCGCAACCCGCTGCTCGAAGCCGCGCGCG
TGCTGCTGCGGGCGCTCGCCGACATGCCCGAGCGGCTCGATCGCGACGACATTCCGCAATTGCGCCTGCTGCTGGAACAG
GAGGTGCGCCTGTTCCAGCGGCTCTGCGAACAGGCGAACATCCGGCGCGACCACATGCTCGGCGCGCGCTACTGCCTGTG
CACCGCGCTCGACGAGGCGGCGATGCAGACGTCGTGGGCACAATCGGCGAGCGGCAATCTCGGCACGTGGATCAGCGAGG
GGCTCGCGACGTCGTTCCACGAGGATCGCCAGGGCGGAGACAAGGTCTATCTGCTGATCGGCCGGCTGATGAATTCGCCG
CACGAGCACATCGACCTGCTCGAAGTCATCTATCGAATCTTGAGCCTCGGCTTCGAGGGCCGCTACCGTTACGAAGCCGA
CGGCCAGCGCAAGCACGAGACCGTGCGCCAGCGGCTCTACAACGAGATCGCATCGCAGCGCGGGCCGGTGTCGGTCGCGC
TGTCGCCGCACTGGCAGCCCGGCCCCCGCAGCAGGAGCGCGCCGTTTCGCGATTTCCCCGCGTGGGTCACGGCCGCCGTG
CTGTCGCTGATCGCGCTCGGGCTGTTCGGCTGCTTCAAGTACGCGCTGTCGACGCGCAGCGCCGACGTGCAGCAGCGGAT
CGCCGCGATCGCGCGGATGGCGCCGCCCGCCGCGCCGGCCGAGCTGCGCCTCGCGACGCTGCTCGCCGGCGAGATCGCGG
CGGGCACGCTCAGCGTCGAGGAAAACGCGCGCCGCAGCTCGGTGACGTTCCGCGGCGACGCGATGTTCGCGCCGGGCGCG
GCCGGCGTGAACCCGGCGATGGGGCCGCTCATCCGGAAAATCGCGGCCGAGATCGCGAAGGTGCCGGGCAAGGTGACGGT
GCGCGGCTACACCGACAATCAGCCGATCAAAAGCCGCCAGTTCGCGTCGAACGAGGCGCTATCCGAAGAGCGCGCGACGC
AGGTCATGCAGATGCTCCAGAGCGCGGGCGTGCCCGCGAGCCGCCTCGAGGCGCTCGGCAAGGGCGGCGCCGAGCCGATC
GGCGACAACCGGACCCCGCAGGGCCGCGCGCTGAACCGCCGCGTCGAAATCACGGTCGCGCGCTGA