Detailed information of component protein


Summary

Component ID T6CP013072
Component protein type TssM
T6SS ID (Type) T6SS01293 experimental (Type i3)
Strain Burkholderia pseudomallei K96243
Replicon chromosome II
Sequence Protein sequence (1303 a.a.); Nucleotide sequence (3909 bp)
Reference
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) AQ15_5060
Coordinate (Strand) 1920770..1924678 (+)
NCBI ID 772958378
GenBank CP009537
Uniprot ID Q63P43_BURPS
KEGG ID bps:BPSS0103
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 5.3e-89 265..538
IcmF-related PF06761.14 1.4e-75 590..912
IcmF_C PF06744.14 6.6e-24 1179..1281

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-11 MSYLKRFLRFV
Transmembrane helix 12-34 FSWQMLACIAVLLVCAAVWFVGP
Outside 35-43 LLAFDELRP
Transmembrane helix 44-66 LAGVVVRVAVIVLLVALLAFWLL
Inside 67-72 RWPLSP
Transmembrane helix 73-91 IGVAALCLLIWHAGPLFAF
Outside 92-100 GDHRPFGPA
Transmembrane helix 101-123 WVRVLIVAAMLFCYAVYGLYRLW
Inside 124-532 RAVRTNDALLKRILEPSAGKPDAAAQANIRAVSVAVSKAIGQLKRLRGGAFGWRRLLESGRYLYELPWYMVVGAPGAGKSAAIARSGLKFPLADQMEASSERARGGTANCEWWFANEAVLIDTAGRYVRHEVPGDEEATVANGAEWKGFLGLLRKHRPRAPVNGVVLSVSVEELVGRTPAERTAHAATLRARLGELHQELGIHFPVYVIVTKLDLLPGFPEYFQSLTAEGRTQIWGFTLPYDAENRRSAVGALREHCADELKRLEMRIDAGLNNRLLEEYENDRRKRLYALPQEFRSLSAALSDMLALVFLDSRYDDAQLQNTLRGVYFTSAEQTDQVLAADRETILQRLKRQLGRMLGGDAGAQTRDSGAVSGSRGYFLRDVFQHVIVPEAHLVRPNVRWEVRFRLMR
Transmembrane helix 533-552 WAGHLLAVALLVWLASALTV
Outside 553-1302 SFDNNRGYLDAISEKTAALAARVNAYNKAPKPAQIGGVLDGARDLPQHGNLDLDAPGASFRYGLYVAPGIVDASDATYRSLLRRSLLPQIVRRVEDALSAQIDAKHADEVYRTLTVYLMLDDAARHDAKAVKDWVMRDWERSDSAAEMGGRNLMARHLDALFVDARPFEPSGHRNAALVQRARLFLNANPAPRRLYERAMAAIEKEAPENFTLARAVGLQGAGIFRLADGSRFQRGMPGLYTYDGYHQVFSARLPEFLARAQSDDAWVMGNAGPAARWGDAIRNTQAVAGRSSLADDIRRQYLTDYGNYWQQYLADIRPASSGEDGAGGTLAFDLATLRALAAPDSPLVRLARAVVRETSLSVVDAREDASLTDAALSSLGRRSGAAKEVADGAQKLAARRPEQRLEKELVDNRFAALREVVTGQADTGSGPAMTDVPVAAGGRALQLDAILTLINEQYTRLVVADNALSSQSMPPALDIGTTLQMEAEKLPAPLRTVLGGIATQAADKVGREVGSLLAMQVDSSVGNACRAAVDGKYPFARSAQEVDIEDFNRLFAAGGLFDAFFQKALAPHVDTHSKPWRYKALNPGMPPIRGPSLEPFERAAAIRDVFFREPGAKRMAWRMDAKVASIDPEITELIVDVDGQSQRYVHGPVLPFSVNWPGPRGGAIAEITAKPRIRPDTSTIATTGPWALFRLIERGRLTGTTSASRLMLDFDFDGRHAALELRTNGQMNPLTSGLLTHFRCPGSVG



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8850 - -



  Protein sequence: 1303 a.a.    

>T6CP013072 CP009537:1920770-1924678 [Burkholderia pseudomallei K96243] [TssM]
MSYLKRFLRFVFSWQMLACIAVLLVCAAVWFVGPLLAFDELRPLAGVVVRVAVIVLLVALLAFWLLRWPLSPIGVAALCL
LIWHAGPLFAFGDHRPFGPAWVRVLIVAAMLFCYAVYGLYRLWRAVRTNDALLKRILEPSAGKPDAAAQANIRAVSVAVS
KAIGQLKRLRGGAFGWRRLLESGRYLYELPWYMVVGAPGAGKSAAIARSGLKFPLADQMEASSERARGGTANCEWWFANE
AVLIDTAGRYVRHEVPGDEEATVANGAEWKGFLGLLRKHRPRAPVNGVVLSVSVEELVGRTPAERTAHAATLRARLGELH
QELGIHFPVYVIVTKLDLLPGFPEYFQSLTAEGRTQIWGFTLPYDAENRRSAVGALREHCADELKRLEMRIDAGLNNRLL
EEYENDRRKRLYALPQEFRSLSAALSDMLALVFLDSRYDDAQLQNTLRGVYFTSAEQTDQVLAADRETILQRLKRQLGRM
LGGDAGAQTRDSGAVSGSRGYFLRDVFQHVIVPEAHLVRPNVRWEVRFRLMRWAGHLLAVALLVWLASALTVSFDNNRGY
LDAISEKTAALAARVNAYNKAPKPAQIGGVLDGARDLPQHGNLDLDAPGASFRYGLYVAPGIVDASDATYRSLLRRSLLP
QIVRRVEDALSAQIDAKHADEVYRTLTVYLMLDDAARHDAKAVKDWVMRDWERSDSAAEMGGRNLMARHLDALFVDARPF
EPSGHRNAALVQRARLFLNANPAPRRLYERAMAAIEKEAPENFTLARAVGLQGAGIFRLADGSRFQRGMPGLYTYDGYHQ
VFSARLPEFLARAQSDDAWVMGNAGPAARWGDAIRNTQAVAGRSSLADDIRRQYLTDYGNYWQQYLADIRPASSGEDGAG
GTLAFDLATLRALAAPDSPLVRLARAVVRETSLSVVDAREDASLTDAALSSLGRRSGAAKEVADGAQKLAARRPEQRLEK
ELVDNRFAALREVVTGQADTGSGPAMTDVPVAAGGRALQLDAILTLINEQYTRLVVADNALSSQSMPPALDIGTTLQMEA
EKLPAPLRTVLGGIATQAADKVGREVGSLLAMQVDSSVGNACRAAVDGKYPFARSAQEVDIEDFNRLFAAGGLFDAFFQK
ALAPHVDTHSKPWRYKALNPGMPPIRGPSLEPFERAAAIRDVFFREPGAKRMAWRMDAKVASIDPEITELIVDVDGQSQR
YVHGPVLPFSVNWPGPRGGAIAEITAKPRIRPDTSTIATTGPWALFRLIERGRLTGTTSASRLMLDFDFDGRHAALELRT
NGQMNPLTSGLLTHFRCPGSVG*

  Nucleotide sequence: 3909 bp    

>T6CP013072 CP009537:1920770-1924678 [Burkholderia pseudomallei K96243] [TssM]
ATGTCTTACCTGAAACGCTTCTTGAGATTCGTCTTTTCGTGGCAGATGCTCGCGTGCATCGCGGTGCTGCTTGTTTGCGC
CGCCGTATGGTTCGTCGGCCCCCTGCTCGCGTTCGACGAGTTGCGGCCGCTCGCGGGCGTCGTCGTGCGGGTCGCCGTCA
TCGTCCTTCTCGTGGCGCTCCTTGCGTTCTGGCTGTTGCGCTGGCCGCTCAGCCCGATCGGCGTCGCCGCACTGTGTCTG
CTGATCTGGCACGCGGGGCCGCTCTTCGCGTTCGGCGATCACCGCCCGTTCGGGCCCGCGTGGGTCCGCGTGCTGATCGT
CGCCGCGATGCTCTTCTGCTACGCCGTGTATGGTCTCTATCGCCTGTGGCGGGCGGTTCGGACGAACGACGCATTGCTCA
AGCGCATTCTCGAGCCGTCGGCGGGCAAGCCGGACGCGGCGGCCCAGGCGAACATCCGTGCGGTGAGCGTGGCGGTGTCG
AAGGCGATCGGTCAACTGAAGCGCTTGCGCGGCGGTGCGTTCGGCTGGCGGCGCTTGCTCGAAAGCGGGCGCTACCTGTA
TGAACTGCCGTGGTACATGGTGGTCGGCGCGCCTGGTGCGGGCAAGAGCGCGGCGATCGCGCGTTCCGGCCTGAAGTTTC
CGCTCGCGGATCAGATGGAGGCATCGTCCGAGCGCGCGCGGGGCGGCACGGCCAATTGCGAATGGTGGTTCGCCAACGAG
GCCGTGTTGATCGACACGGCGGGCCGTTACGTGCGGCACGAGGTGCCCGGCGACGAAGAGGCGACAGTTGCCAACGGGGC
GGAGTGGAAGGGCTTTCTCGGTCTGCTTCGCAAACATCGGCCGCGCGCGCCGGTCAATGGCGTGGTCCTGTCGGTCAGCG
TCGAGGAGCTCGTCGGGCGCACGCCGGCCGAGCGCACCGCGCACGCAGCCACGCTGCGCGCCCGCCTGGGCGAGTTGCAT
CAGGAACTGGGCATTCATTTCCCGGTATACGTGATCGTCACGAAACTCGATTTGCTGCCCGGCTTTCCGGAGTATTTCCA
GTCGTTGACGGCCGAAGGGCGGACGCAGATCTGGGGATTCACGCTGCCCTACGACGCGGAGAACAGGCGAAGCGCGGTCG
GCGCGCTGCGCGAGCATTGCGCCGACGAGCTGAAGCGGCTGGAGATGCGTATCGACGCGGGGCTGAACAACCGTCTGCTC
GAAGAGTACGAAAACGATCGCCGCAAGCGGCTGTATGCGTTGCCGCAGGAGTTCCGGAGCCTGTCGGCCGCGCTGTCGGA
CATGCTCGCCCTCGTGTTTCTCGATTCACGCTACGACGACGCCCAGTTGCAGAACACGCTGCGCGGCGTCTACTTCACGA
GCGCCGAGCAGACGGATCAGGTATTGGCCGCGGATCGCGAAACCATCCTGCAGCGCTTGAAGCGCCAGCTCGGCAGAATG
CTCGGCGGCGATGCCGGCGCGCAGACGCGTGACAGCGGCGCGGTGTCGGGCAGCCGCGGCTACTTCCTGCGCGATGTGTT
CCAGCATGTGATCGTGCCGGAGGCGCATCTGGTTCGGCCGAACGTCAGGTGGGAGGTGCGTTTTCGCCTGATGCGCTGGG
CCGGGCATCTGCTCGCCGTCGCATTGCTGGTCTGGCTGGCGAGCGCGCTGACGGTGAGCTTCGACAACAATCGCGGCTAC
CTGGACGCGATCTCGGAAAAGACAGCCGCGCTCGCCGCACGAGTCAACGCGTACAACAAGGCGCCGAAGCCGGCGCAGAT
CGGGGGTGTACTGGATGGCGCGCGCGATCTTCCTCAGCATGGCAATCTCGATCTCGACGCGCCCGGCGCGAGCTTCCGCT
ACGGCCTGTATGTCGCACCCGGCATCGTCGACGCGTCCGACGCGACTTATCGGAGCCTGCTGCGCCGGAGCCTTCTGCCG
CAGATCGTGCGGCGCGTCGAAGATGCACTGAGCGCGCAAATCGATGCGAAGCATGCGGACGAGGTCTATCGCACGCTGAC
GGTCTACTTGATGCTCGACGACGCCGCGCGCCATGACGCGAAGGCGGTGAAAGACTGGGTCATGCGCGACTGGGAGCGCT
CGGATAGCGCGGCCGAGATGGGGGGGCGCAATTTGATGGCGCGGCACCTGGACGCGCTGTTCGTCGATGCTCGGCCTTTC
GAGCCGAGCGGGCACCGGAACGCCGCACTCGTTCAGCGCGCCCGGCTTTTCCTCAACGCGAACCCGGCCCCGAGGCGGCT
GTACGAGCGTGCGATGGCCGCGATCGAGAAGGAGGCGCCGGAGAACTTCACGCTCGCGCGTGCGGTCGGGCTTCAGGGCG
CGGGCATCTTTCGGCTCGCCGACGGCTCGCGGTTTCAACGCGGGATGCCCGGCTTGTATACGTACGATGGCTACCACCAG
GTATTCAGCGCCCGGTTGCCCGAATTTCTCGCCCGTGCGCAAAGCGACGACGCATGGGTGATGGGGAACGCCGGCCCCGC
CGCGCGATGGGGCGACGCGATTCGGAACACGCAGGCCGTTGCGGGTCGGTCTTCGCTTGCCGACGACATTCGTCGCCAGT
ACCTGACGGATTACGGCAACTATTGGCAACAGTATCTGGCGGACATTCGGCCCGCGTCGTCCGGAGAGGACGGCGCGGGC
GGCACGCTCGCATTCGATCTCGCGACGCTGCGCGCGCTCGCCGCCCCCGATTCACCGCTCGTGAGACTGGCGCGCGCCGT
CGTTCGCGAAACATCGCTGTCGGTCGTCGACGCACGGGAAGACGCGTCGTTGACCGACGCCGCGCTTTCGTCGCTCGGGC
GACGATCCGGCGCGGCGAAGGAGGTTGCCGACGGCGCGCAGAAACTGGCGGCGCGGCGGCCGGAGCAGCGCCTCGAGAAG
GAGCTGGTGGACAACCGCTTCGCGGCGCTGCGCGAGGTGGTGACGGGGCAGGCCGACACGGGAAGCGGGCCGGCGATGAC
GGACGTGCCGGTCGCGGCGGGGGGCCGGGCGCTGCAGCTCGACGCGATCCTCACGCTGATCAACGAACAGTACACGCGCC
TCGTCGTTGCCGACAACGCGCTCTCCTCGCAGAGCATGCCGCCCGCGCTGGACATCGGCACCACCTTGCAAATGGAGGCG
GAGAAACTGCCCGCGCCGCTGCGCACGGTGCTGGGCGGCATCGCGACGCAAGCCGCCGACAAAGTCGGGCGCGAAGTGGG
TTCGCTGCTCGCGATGCAGGTGGACTCGAGTGTCGGCAACGCGTGTCGCGCCGCCGTCGACGGCAAGTATCCGTTCGCGC
GCAGCGCGCAGGAAGTCGACATCGAGGATTTCAACCGCCTCTTCGCCGCGGGCGGCCTGTTCGACGCGTTTTTTCAGAAG
GCGCTCGCGCCGCATGTCGACACCCATTCGAAGCCGTGGCGTTACAAGGCGTTGAATCCCGGCATGCCGCCGATCCGGGG
GCCGAGCCTCGAGCCGTTCGAGCGCGCCGCCGCGATTCGCGACGTGTTCTTTCGTGAACCGGGCGCAAAGCGGATGGCGT
GGAGGATGGATGCGAAGGTGGCGTCGATCGACCCTGAGATCACCGAGCTCATCGTCGACGTCGACGGCCAGTCGCAGCGC
TATGTCCACGGCCCCGTGCTGCCGTTCTCCGTGAACTGGCCCGGGCCGCGCGGCGGCGCGATCGCCGAGATCACCGCGAA
GCCGCGGATTCGTCCCGATACGTCGACGATCGCGACGACGGGGCCCTGGGCGCTGTTTCGGCTGATCGAGCGCGGGCGAT
TGACGGGCACCACGTCGGCGAGCCGGTTGATGCTGGATTTCGATTTCGACGGTCGGCACGCGGCCCTGGAGCTCAGGACG
AACGGCCAGATGAATCCGTTGACGAGCGGCTTGCTGACGCATTTTCGCTGTCCGGGGAGCGTCGGTTGA