Detailed information of component protein


Summary

Component ID T6CP013071
Component protein type TssL
T6SS ID (Type) T6SS01293 experimental (Type i3)
Strain Burkholderia pseudomallei K96243
Replicon chromosome II
Sequence Protein sequence (434 a.a.); Nucleotide sequence (1302 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) AQ15_5059
Coordinate (Strand) 1919454..1920755 (+)
NCBI ID 772956999
GenBank CP009537
Uniprot ID Q63P44_BURPS
KEGG ID bps:BPSS0102
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 3e-61 63..266
OmpA PF00691.22 2.3e-17 327..425
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-244 MNSGAFDEAAFDFLVSDEGGANVAADRARSAAKAAGAEPARDRIDASVNVQHRLDEVRAAANPLLEAATPLLRMLADMPATLDTSEAVASLRTLLVREVALFQNLCEKADLPWKHMAVVRYCLCTALDEAANRTRWGGGGVWASQSLLITYEGEVDGGEKFFLLIGRMATDPQEYVDILEILYRVLGLGFEGRYSVVADGRRHLEQIRQRLWTLITGARDAIQPELSLRWRGAEPGRLPLLRSV
Transmembrane helix 245-264 PAWASGALVFLLLFGLFAFY
Inside 265-433 QYRLLTERNALEARILAIAKDEPATPPQRLRLSILLKNEIARGLLTVDEDDRRSVVVFRGDAMFRSGESRVLPEIEPVLDKVAREVARVGGRVTVTGHSDNQPIRRADIPNNLVLSEKRAAYVAQILVQRGTPADRIRSEGRGDAQPVAENATPAGRSRNRRVEIAVTL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9604 - -


  Protein sequence: 434 a.a.    

>T6CP013071 CP009537:1919454-1920755 [Burkholderia pseudomallei K96243] [TssL]
MNSGAFDEAAFDFLVSDEGGANVAADRARSAAKAAGAEPARDRIDASVNVQHRLDEVRAAANPLLEAATPLLRMLADMPA
TLDTSEAVASLRTLLVREVALFQNLCEKADLPWKHMAVVRYCLCTALDEAANRTRWGGGGVWASQSLLITYEGEVDGGEK
FFLLIGRMATDPQEYVDILEILYRVLGLGFEGRYSVVADGRRHLEQIRQRLWTLITGARDAIQPELSLRWRGAEPGRLPL
LRSVPAWASGALVFLLLFGLFAFYQYRLLTERNALEARILAIAKDEPATPPQRLRLSILLKNEIARGLLTVDEDDRRSVV
VFRGDAMFRSGESRVLPEIEPVLDKVAREVARVGGRVTVTGHSDNQPIRRADIPNNLVLSEKRAAYVAQILVQRGTPADR
IRSEGRGDAQPVAENATPAGRSRNRRVEIAVTL*

  Nucleotide sequence: 1302 bp    

>T6CP013071 CP009537:1919454-1920755 [Burkholderia pseudomallei K96243] [TssL]
ATGAATTCGGGCGCCTTTGACGAAGCCGCGTTCGATTTTCTCGTATCGGACGAAGGCGGAGCGAACGTCGCGGCCGATCG
CGCGCGATCGGCCGCGAAGGCCGCGGGCGCGGAGCCGGCGCGCGATCGGATCGACGCGTCGGTCAACGTCCAGCATCGCC
TCGACGAAGTTCGCGCGGCGGCCAATCCGCTGCTCGAAGCCGCGACGCCTTTGCTGCGGATGCTGGCGGACATGCCTGCG
ACGCTAGACACCTCCGAAGCCGTCGCGAGCCTTCGCACCCTGCTCGTGCGAGAGGTCGCGCTGTTCCAGAACTTGTGCGA
GAAGGCTGATTTGCCTTGGAAGCACATGGCGGTCGTGCGTTATTGCCTGTGCACGGCACTGGACGAGGCGGCCAATCGCA
CGCGCTGGGGCGGCGGCGGCGTATGGGCTTCGCAGAGCTTGCTGATTACATACGAAGGCGAGGTCGACGGAGGCGAGAAG
TTCTTCCTGTTGATCGGACGCATGGCGACGGATCCGCAGGAGTACGTCGATATTCTCGAGATTCTCTATCGCGTGCTGGG
TCTCGGGTTCGAAGGGCGCTACAGCGTCGTTGCGGACGGCCGGCGGCATCTCGAGCAGATTCGCCAGCGTTTGTGGACGC
TCATCACCGGCGCGCGCGACGCGATTCAGCCGGAATTGTCGCTGCGCTGGCGCGGAGCGGAGCCGGGCAGGCTTCCGCTG
TTGCGCAGCGTGCCCGCATGGGCGAGCGGTGCGCTCGTCTTCCTGCTGTTGTTCGGTCTCTTCGCGTTCTATCAGTATCG
GTTGCTCACCGAACGCAATGCGCTCGAGGCGCGCATCCTGGCGATCGCGAAGGACGAGCCGGCGACGCCACCGCAGCGTT
TGCGCCTGTCGATCCTGCTCAAGAACGAAATTGCGCGCGGTCTACTGACGGTCGACGAAGACGATAGGCGCAGCGTGGTC
GTGTTCCGCGGCGATGCGATGTTCCGCTCCGGCGAGAGCCGCGTGCTTCCCGAGATCGAGCCGGTGCTCGACAAGGTGGC
GCGGGAAGTGGCCCGCGTCGGCGGCCGGGTCACGGTCACGGGGCACTCCGACAACCAGCCGATTCGGCGCGCCGACATCC
CGAACAATCTCGTGTTGTCGGAAAAACGCGCGGCTTACGTCGCGCAAATCCTGGTCCAGCGAGGCACTCCGGCCGATCGT
ATTCGCTCGGAGGGCCGGGGCGACGCGCAGCCCGTCGCGGAAAACGCGACGCCTGCTGGCCGATCCCGCAACCGCCGCGT
CGAAATCGCGGTCACGCTGTAA