Detailed information of component protein
Summary
| Component ID | T6CP013069 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS01293  (Type i3) |
| Strain | Burkholderia pseudomallei K96243 |
| Replicon | chromosome II |
| Sequence | Protein sequence (181 a.a.); Nucleotide sequence (543 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | AQ15_5057 |
| Coordinate (Strand) | 1917560..1918102 (+) |
| NCBI ID | 772958673 |
| GenBank | CP009537 |
| Uniprot ID | UPI00025C24F1 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 1.3e-34 | 44..169 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-180 | MAFPFFFRREAARRTAVKRVVAAMVCTSLAACASLPARKETADLIVKIKVSEGANPDEHQRPAPVMVRLYELKSAGAFENADFFTLQSDSRKVLGDDAIATDEFVMRPGDTRDIHRRADSAGTSVGVLVGYRELGKSVWRAVHKLRPAPEDAWYRAFTPRTKIKLNVDVGQQTVTITELE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.8309 | pos: 31-32. LAA-CA. | MAFPFFFRREAARRTAVKRVVAAMVCTSLAA |
Protein sequence: 181 a.a.
>T6CP013069 CP009537:1917560-1918102 [Burkholderia pseudomallei K96243] [TssJ]
MAFPFFFRREAARRTAVKRVVAAMVCTSLAACASLPARKETADLIVKIKVSEGANPDEHQRPAPVMVRLYELKSAGAFEN
ADFFTLQSDSRKVLGDDAIATDEFVMRPGDTRDIHRRADSAGTSVGVLVGYRELGKSVWRAVHKLRPAPEDAWYRAFTPR
TKIKLNVDVGQQTVTITELE*
MAFPFFFRREAARRTAVKRVVAAMVCTSLAACASLPARKETADLIVKIKVSEGANPDEHQRPAPVMVRLYELKSAGAFEN
ADFFTLQSDSRKVLGDDAIATDEFVMRPGDTRDIHRRADSAGTSVGVLVGYRELGKSVWRAVHKLRPAPEDAWYRAFTPR
TKIKLNVDVGQQTVTITELE*
Nucleotide sequence: 543 bp
>T6CP013069 CP009537:1917560-1918102 [Burkholderia pseudomallei K96243] [TssJ]
ATGGCTTTCCCATTTTTCTTTCGCCGCGAAGCGGCCAGGCGAACCGCCGTCAAGCGCGTTGTGGCGGCAATGGTATGCAC
ATCGCTCGCGGCTTGCGCAAGCTTGCCGGCACGCAAGGAAACAGCCGATCTGATCGTGAAGATCAAGGTGAGCGAAGGCG
CCAATCCGGATGAGCATCAGCGCCCGGCGCCCGTGATGGTGCGGCTGTACGAGCTGAAATCGGCCGGCGCGTTCGAGAAC
GCCGATTTTTTCACGCTACAGAGCGACAGCCGCAAGGTGTTGGGTGACGACGCGATCGCGACGGATGAATTCGTGATGCG
CCCGGGCGACACCCGGGATATTCATCGGCGTGCCGATTCCGCGGGGACGTCGGTCGGCGTGCTGGTCGGTTATCGAGAGC
TCGGCAAATCGGTGTGGCGGGCGGTCCATAAGCTGCGGCCGGCTCCGGAGGATGCATGGTACCGGGCATTCACGCCAAGG
ACGAAAATCAAGTTGAACGTCGATGTCGGGCAGCAGACGGTGACGATTACAGAATTGGAATGA
ATGGCTTTCCCATTTTTCTTTCGCCGCGAAGCGGCCAGGCGAACCGCCGTCAAGCGCGTTGTGGCGGCAATGGTATGCAC
ATCGCTCGCGGCTTGCGCAAGCTTGCCGGCACGCAAGGAAACAGCCGATCTGATCGTGAAGATCAAGGTGAGCGAAGGCG
CCAATCCGGATGAGCATCAGCGCCCGGCGCCCGTGATGGTGCGGCTGTACGAGCTGAAATCGGCCGGCGCGTTCGAGAAC
GCCGATTTTTTCACGCTACAGAGCGACAGCCGCAAGGTGTTGGGTGACGACGCGATCGCGACGGATGAATTCGTGATGCG
CCCGGGCGACACCCGGGATATTCATCGGCGTGCCGATTCCGCGGGGACGTCGGTCGGCGTGCTGGTCGGTTATCGAGAGC
TCGGCAAATCGGTGTGGCGGGCGGTCCATAAGCTGCGGCCGGCTCCGGAGGATGCATGGTACCGGGCATTCACGCCAAGG
ACGAAAATCAAGTTGAACGTCGATGTCGGGCAGCAGACGGTGACGATTACAGAATTGGAATGA