Detailed information of component protein
Summary
| Component ID | T6CP013065 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS01292  (Type i3) |
| Strain | Burkholderia pseudomallei K96243 |
| Replicon | chromosome II |
| Sequence | Protein sequence (173 a.a.); Nucleotide sequence (519 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | AQ15_4692 |
| Coordinate (Strand) | 1477860..1478378 (-) |
| NCBI ID | 772957978 |
| GenBank | CP009537 |
| Uniprot ID | Q63IG6_BURPS |
| KEGG ID | bps:BPSS2107 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 5.5e-36 | 31..158 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-172 | MRPRFSVSVVLGCALLLAGCGATERSVAVPYSITLDVAPDVNPDLNRKPSPIVLKVFQLKTASAFESADFFSLQDKPQSVLGADLLGVDRIILRPGDARTLHYRGNVDAGAIGIVAEYRVLEKNRWRMTVPLPRAKQLNLYRFWQTSPGEMKLQVAVKNGGIGLGGDSRVRQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9983 | pos: 19-20. LAG-CG. | MRPRFSVSVVLGCALLLAG |
Protein sequence: 173 a.a.
>T6CP013065 CP009537:c1478378-1477860 [Burkholderia pseudomallei K96243] [TssJ]
MRPRFSVSVVLGCALLLAGCGATERSVAVPYSITLDVAPDVNPDLNRKPSPIVLKVFQLKTASAFESADFFSLQDKPQSV
LGADLLGVDRIILRPGDARTLHYRGNVDAGAIGIVAEYRVLEKNRWRMTVPLPRAKQLNLYRFWQTSPGEMKLQVAVKNG
GIGLGGDSRVRQ*
MRPRFSVSVVLGCALLLAGCGATERSVAVPYSITLDVAPDVNPDLNRKPSPIVLKVFQLKTASAFESADFFSLQDKPQSV
LGADLLGVDRIILRPGDARTLHYRGNVDAGAIGIVAEYRVLEKNRWRMTVPLPRAKQLNLYRFWQTSPGEMKLQVAVKNG
GIGLGGDSRVRQ*
Nucleotide sequence: 519 bp
>T6CP013065 CP009537:c1478378-1477860 [Burkholderia pseudomallei K96243] [TssJ]
ATGCGACCGCGGTTCAGTGTTTCCGTCGTTCTGGGATGCGCGCTGCTGCTCGCCGGATGCGGGGCGACCGAGCGTTCCGT
TGCGGTGCCGTATTCGATCACGCTCGACGTCGCGCCCGACGTCAATCCGGACCTCAATCGCAAGCCGTCGCCGATCGTGC
TGAAGGTGTTCCAGCTGAAGACGGCGTCCGCGTTCGAAAGCGCCGATTTCTTCTCGCTGCAGGACAAGCCGCAGAGCGTG
CTCGGCGCGGATCTGCTCGGCGTCGACCGGATCATCCTGCGCCCGGGGGATGCGCGCACGCTGCACTATCGCGGCAACGT
CGACGCGGGCGCGATCGGCATCGTCGCCGAATATCGCGTGCTCGAGAAGAACCGCTGGCGGATGACGGTGCCGCTGCCGC
GCGCGAAGCAACTGAACCTGTACCGATTCTGGCAAACCTCGCCGGGCGAGATGAAGCTGCAGGTCGCCGTGAAGAACGGC
GGCATCGGCCTCGGCGGCGATTCGCGAGTACGCCAATGA
ATGCGACCGCGGTTCAGTGTTTCCGTCGTTCTGGGATGCGCGCTGCTGCTCGCCGGATGCGGGGCGACCGAGCGTTCCGT
TGCGGTGCCGTATTCGATCACGCTCGACGTCGCGCCCGACGTCAATCCGGACCTCAATCGCAAGCCGTCGCCGATCGTGC
TGAAGGTGTTCCAGCTGAAGACGGCGTCCGCGTTCGAAAGCGCCGATTTCTTCTCGCTGCAGGACAAGCCGCAGAGCGTG
CTCGGCGCGGATCTGCTCGGCGTCGACCGGATCATCCTGCGCCCGGGGGATGCGCGCACGCTGCACTATCGCGGCAACGT
CGACGCGGGCGCGATCGGCATCGTCGCCGAATATCGCGTGCTCGAGAAGAACCGCTGGCGGATGACGGTGCCGCTGCCGC
GCGCGAAGCAACTGAACCTGTACCGATTCTGGCAAACCTCGCCGGGCGAGATGAAGCTGCAGGTCGCCGTGAAGAACGGC
GGCATCGGCCTCGGCGGCGATTCGCGAGTACGCCAATGA