SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP012975
Component protein type	TssI
T6SS ID (Type)	T6SS00948 (Type i1)
Strain	Aeromonas hydrophila NJ-35
Replicon	chromosome
Sequence	Protein sequence (744 a.a.); Nucleotide sequence (2232 bp)
Reference	[1] Wang N, Liu J, Pang M, et al. Diverse roles of Hcp family proteins in the environmental fitness and pathogenicity of Aeromonas hydrophila Chinese epidemic strain NJ-35. Appl Microbiol Biotechnol. 2018 Aug;102(16):7083-7095. doi: 10.1007/s00253-018-9116-0. Epub 2018 Jun 3. PMID: 29862449 [2] Wang N, Wu Y, Pang M, et al. Protective efficacy of recombinant hemolysin co-regulated protein (Hcp) of Aeromonas hydrophila in common carp (Cyprinus carpio). Fish Shellfish Immunol. 2015 Oct;46(2):297-304. doi: 10.1016/j.fsi.2015.06.019. Epub 2015 Jun 18. PMID: 26093203

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	U876_13605
Coordinate (Strand)	2972993..2975224 (-)
NCBI ID	827372813
GenBank	CP006870
Uniprot ID	UPI000588D141
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	5.7e-90	30..327
Phage_base_V	PF04717.14	1.7e-14	389..456

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-743	MADSTGLQFTVKVGALPESTFVVAEFALDEALNRPFNLRLELASAQPDIDFGAVLDQPCELLVWYNGELQRRVCGVVSDFAQGDSGFRRTRYQLLVQPALWRLSLRQNSRIFQAQKPDEILSILLQEHGITDYAFALRNEHAKREYCVQYRETDLDFVNRLAAEEGMFYFHEFEAGKHRIVFADDAAALTQGPELFFNLGNRSLEQGPYVRQFHYREAVRPSDVELKDYSFKTPAYGLSHKKQGSELEHQRDTYQHFDYPGRYKQDGSGKAFAQHRLDALRNDAVAGQAKSNCAALQPGQSFSLTEHPNGSLNTDWQIVRIQHTGEQPQALEEEGGSGPTVYHNEFGVVKASSTWRARIGSPEAPHKPMVDGPQIAMVVGPDGEEIYCDEHGRVKLQFPWDRYGSSNDQSSCWVRVSQGWAGGQYGMMAIPRIGHEVIVSFLEGDPDQPIVTGRTYHATNRPPYELPANKTRTVLRTETHQGEGFNELRFEDQAGQEEIYIHGQKDLNVLIENDAAWHIKHDEHRDIDNERVTRVRKVPGEEGAPPSFGNDHLTVEGEKRDHIKADYSLTVDTSMHQKLGQSWLTQAGQEVHVKAGAKVVLEAGSEITVNVGGCFIKVDGGGVTLVGPTIKMNSGGNAGSGSGWAGKVPKSMEGMLDSSHTRWMKFYHLDSELMPLAGTPYKAVLSDGSVREGTLDGEGMALLEDVPAGTASVTYDLQDTFADLPRESISALTGHLDSLSDEG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9890	-	-

Protein sequence: 744 a.a.

						>T6CP012975 CP006870:c2975224-2972993 [Aeromonas hydrophila NJ-35] [TssI]
MADSTGLQFTVKVGALPESTFVVAEFALDEALNRPFNLRLELASAQPDIDFGAVLDQPCELLVWYNGELQRRVCGVVSDF
AQGDSGFRRTRYQLLVQPALWRLSLRQNSRIFQAQKPDEILSILLQEHGITDYAFALRNEHAKREYCVQYRETDLDFVNR
LAAEEGMFYFHEFEAGKHRIVFADDAAALTQGPELFFNLGNRSLEQGPYVRQFHYREAVRPSDVELKDYSFKTPAYGLSH
KKQGSELEHQRDTYQHFDYPGRYKQDGSGKAFAQHRLDALRNDAVAGQAKSNCAALQPGQSFSLTEHPNGSLNTDWQIVR
IQHTGEQPQALEEEGGSGPTVYHNEFGVVKASSTWRARIGSPEAPHKPMVDGPQIAMVVGPDGEEIYCDEHGRVKLQFPW
DRYGSSNDQSSCWVRVSQGWAGGQYGMMAIPRIGHEVIVSFLEGDPDQPIVTGRTYHATNRPPYELPANKTRTVLRTETH
QGEGFNELRFEDQAGQEEIYIHGQKDLNVLIENDAAWHIKHDEHRDIDNERVTRVRKVPGEEGAPPSFGNDHLTVEGEKR
DHIKADYSLTVDTSMHQKLGQSWLTQAGQEVHVKAGAKVVLEAGSEITVNVGGCFIKVDGGGVTLVGPTIKMNSGGNAGS
GSGWAGKVPKSMEGMLDSSHTRWMKFYHLDSELMPLAGTPYKAVLSDGSVREGTLDGEGMALLEDVPAGTASVTYDLQDT
FADLPRESISALTGHLDSLSDEG*					

Nucleotide sequence: 2232 bp

						>T6CP012975 CP006870:c2975224-2972993 [Aeromonas hydrophila NJ-35] [TssI]
ATGGCAGACAGCACAGGATTACAATTTACCGTCAAGGTGGGGGCTCTGCCCGAGAGTACTTTCGTGGTGGCCGAGTTTGC
GCTGGATGAAGCGCTCAACCGGCCGTTCAACCTGCGCCTGGAGCTGGCGAGTGCCCAGCCCGACATCGACTTTGGCGCCG
TGCTGGACCAGCCGTGCGAGCTGCTGGTGTGGTACAACGGCGAGCTGCAGCGCCGGGTGTGCGGGGTGGTCAGTGACTTC
GCGCAGGGTGACAGCGGCTTTCGCCGCACCCGCTATCAGCTGCTGGTGCAGCCGGCCCTGTGGCGGCTGTCGCTTCGCCA
GAACTCCCGCATCTTCCAGGCACAAAAGCCCGATGAAATCCTCAGTATCCTGTTGCAAGAGCACGGCATCACCGACTACG
CCTTCGCCCTGAGAAACGAGCACGCCAAGCGGGAATACTGCGTTCAGTACCGGGAAACCGACCTCGATTTCGTGAACCGA
CTCGCCGCCGAAGAGGGGATGTTCTACTTCCACGAGTTTGAAGCAGGCAAGCACCGCATTGTGTTTGCCGACGACGCGGC
GGCATTGACTCAAGGCCCCGAGCTCTTCTTCAACCTCGGTAACCGCTCGCTCGAACAAGGCCCCTATGTACGGCAGTTCC
ACTACCGGGAGGCGGTGCGCCCGTCGGATGTGGAGCTCAAAGACTACAGCTTCAAAACCCCGGCTTATGGCCTCTCCCAC
AAAAAGCAGGGCAGTGAGCTCGAACACCAGCGCGACACCTACCAGCACTTCGACTATCCGGGCCGCTACAAGCAGGATGG
CAGCGGCAAGGCCTTTGCCCAGCACCGGCTGGATGCGCTGCGCAATGACGCGGTGGCGGGCCAGGCTAAGTCCAACTGCG
CGGCTTTACAGCCGGGGCAGAGCTTCTCACTGACCGAACATCCCAACGGCAGTCTCAACACCGACTGGCAAATCGTGCGT
ATCCAGCACACCGGTGAGCAACCGCAGGCGCTGGAAGAGGAGGGTGGGAGCGGCCCGACCGTCTACCACAACGAATTTGG
GGTGGTGAAAGCGAGCTCCACCTGGCGGGCACGCATCGGCAGCCCGGAAGCCCCTCACAAGCCGATGGTGGATGGCCCGC
AAATCGCCATGGTGGTGGGGCCCGACGGGGAAGAAATTTACTGCGACGAGCACGGCAGGGTGAAGCTGCAGTTCCCGTGG
GACCGCTATGGTTCGAGCAACGACCAGAGCTCCTGCTGGGTGCGGGTCTCGCAAGGCTGGGCGGGCGGTCAGTACGGCAT
GATGGCCATCCCGCGCATCGGCCACGAGGTCATCGTGAGTTTCTTGGAAGGGGACCCGGACCAGCCCATCGTGACCGGTC
GCACCTATCATGCCACCAACCGGCCTCCTTATGAGCTGCCTGCCAACAAGACCCGCACCGTGCTGCGCACCGAAACCCAC
CAGGGTGAAGGCTTCAACGAGCTGCGCTTTGAAGACCAGGCTGGGCAGGAAGAGATTTACATCCACGGTCAGAAAGACCT
GAACGTGCTTATCGAGAACGATGCGGCGTGGCATATCAAGCACGACGAGCATCGGGATATCGACAATGAGCGGGTGACCC
GTGTCAGGAAGGTACCGGGTGAGGAGGGGGCCCCACCCAGTTTTGGCAACGATCATCTGACCGTTGAAGGAGAGAAGCGG
GACCATATCAAGGCTGACTACTCGCTGACCGTGGATACCTCCATGCATCAAAAACTCGGTCAGAGCTGGCTCACCCAGGC
GGGACAGGAAGTGCACGTCAAAGCCGGTGCCAAGGTGGTGCTGGAAGCGGGTTCTGAGATTACCGTCAATGTCGGCGGCT
GCTTTATCAAGGTTGATGGCGGCGGTGTCACTTTGGTCGGCCCCACCATCAAGATGAACTCGGGTGGTAATGCAGGCTCT
GGATCCGGTTGGGCTGGCAAGGTACCCAAGAGCATGGAGGGTATGCTGGACAGCTCTCACACACGCTGGATGAAATTTTA
TCACCTCGACTCCGAGCTGATGCCGCTGGCGGGCACCCCATACAAGGCTGTGCTGAGCGATGGTTCGGTGCGGGAAGGCA
CGCTGGATGGTGAGGGCATGGCGCTGCTCGAGGATGTGCCTGCTGGCACCGCTTCCGTGACCTATGACCTGCAGGACACT
TTTGCCGATCTGCCCCGTGAATCCATCTCGGCCCTCACCGGCCATCTCGACAGTCTGAGCGACGAGGGCTGA					