SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP012969
Component protein type	TssJ
T6SS ID (Type)	T6SS00948 (Type i1)
Strain	Aeromonas hydrophila NJ-35
Replicon	chromosome
Sequence	Protein sequence (172 a.a.); Nucleotide sequence (516 bp)
Reference	[1] Wang N, Liu J, Pang M, et al. Diverse roles of Hcp family proteins in the environmental fitness and pathogenicity of Aeromonas hydrophila Chinese epidemic strain NJ-35. Appl Microbiol Biotechnol. 2018 Aug;102(16):7083-7095. doi: 10.1007/s00253-018-9116-0. Epub 2018 Jun 3. PMID: 29862449 [2] Wang N, Wu Y, Pang M, et al. Protective efficacy of recombinant hemolysin co-regulated protein (Hcp) of Aeromonas hydrophila in common carp (Cyprinus carpio). Fish Shellfish Immunol. 2015 Oct;46(2):297-304. doi: 10.1016/j.fsi.2015.06.019. Epub 2015 Jun 18. PMID: 26093203

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	U876_13550
Coordinate (Strand)	2960810..2961325 (-)
NCBI ID	827372803
GenBank	CP006870
Uniprot ID	UPI0000E69209
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	3.3e-33	42..160

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-171	MIRALMLGAALLALAGCTTMGKMADVAMNPDIQVGSNDSQPTTLGLSLLAEPDVNPNESGEAAPIEFQVVLLAEDSKLLATDYDQVTADVEKALGKNYLDHQDYTLLPGQFKYLPPIKLDEKTRYIGVIARYADPDSAEWRKVIKIKSKGTAYQILVHLRLDEVELQKEEE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9974	pos: 16-17. LAG-CT.	MIRALMLGAALLALAG

Protein sequence: 172 a.a.

						>T6CP012969 CP006870:c2961325-2960810 [Aeromonas hydrophila NJ-35] [TssJ]
MIRALMLGAALLALAGCTTMGKMADVAMNPDIQVGSNDSQPTTLGLSLLAEPDVNPNESGEAAPIEFQVVLLAEDSKLLA
TDYDQVTADVEKALGKNYLDHQDYTLLPGQFKYLPPIKLDEKTRYIGVIARYADPDSAEWRKVIKIKSKGTAYQILVHLR
LDEVELQKEEE*					

Nucleotide sequence: 516 bp

						>T6CP012969 CP006870:c2961325-2960810 [Aeromonas hydrophila NJ-35] [TssJ]
ATGATACGTGCGCTGATGCTGGGCGCCGCGCTGCTGGCCCTGGCAGGTTGTACCACCATGGGCAAGATGGCCGACGTGGC
CATGAACCCGGATATCCAGGTGGGCAGCAATGACAGTCAACCGACGACCCTGGGACTGAGCCTGCTGGCGGAGCCTGACG
TCAACCCCAACGAGAGCGGGGAGGCGGCGCCCATCGAGTTTCAGGTGGTGTTGCTGGCAGAGGATTCCAAGCTGCTCGCC
ACCGACTACGACCAGGTGACCGCGGACGTGGAGAAGGCCCTTGGCAAGAACTACCTGGACCATCAGGACTACACCCTGCT
GCCGGGCCAGTTCAAATACCTGCCCCCCATCAAGCTCGACGAGAAGACCCGCTACATCGGGGTGATCGCACGCTACGCCG
ACCCCGACAGCGCCGAGTGGCGCAAGGTCATCAAGATCAAGAGCAAGGGAACGGCTTATCAAATCCTGGTGCACCTGCGC
CTGGATGAAGTGGAACTGCAAAAAGAAGAAGAATAA					