SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP012961
Component protein type	TssH
T6SS ID (Type)	T6SS00948 (Type i1)
Strain	Aeromonas hydrophila NJ-35
Replicon	chromosome
Sequence	Protein sequence (751 a.a.); Nucleotide sequence (2253 bp)
Reference	[1] Wang N, Liu J, Pang M, et al. Diverse roles of Hcp family proteins in the environmental fitness and pathogenicity of Aeromonas hydrophila Chinese epidemic strain NJ-35. Appl Microbiol Biotechnol. 2018 Aug;102(16):7083-7095. doi: 10.1007/s00253-018-9116-0. Epub 2018 Jun 3. PMID: 29862449 [2] Wang N, Wu Y, Pang M, et al. Protective efficacy of recombinant hemolysin co-regulated protein (Hcp) of Aeromonas hydrophila in common carp (Cyprinus carpio). Fish Shellfish Immunol. 2015 Oct;46(2):297-304. doi: 10.1016/j.fsi.2015.06.019. Epub 2015 Jun 18. PMID: 26093203

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	U876_13455
Coordinate (Strand)	2935924..2938176 (-)
NCBI ID	827372787
GenBank	CP006870
Uniprot ID	UPI0002FAF6B2
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
AAA_2	PF07724.16	1.1e-48	483..643
AAA_lid_9	PF17871.3	2.4e-29	347..450
ClpB_D2-small	PF10431.11	2e-23	650..730
Clp_N	PF02861.22	5.9e-12	13..62
AAA	PF00004.31	2.4e-11	208..298

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-750	MLNKDLEKTLNEAFKLARDERHEFMTVEHLLLALLDNSSAHEALSSCGADVELMRKEISAFISQTTPLIPRDDEDRETQPTLGFQRVLQRAVFHVQSSGNTEVSGANVLVAIFSEQESQAAYFLKKAEISRLDVVNFLSHGVRKDSKPDNSNNNAESDDDVSAAQIESFATNLNQLVLEGRIDPLIGRDQELNRTIQVLCRRRKNNPLLVGEAGVGKTAIAEGLAYRIVKGDVPEVIAGSTIYSLDMGSLLAGTKYRGDFEKRLKVLLKQIERQEGAILFIDEIHTIIGAGAASGGQLDAANLIKPLLSNGLLRCVGSTTYQEYSQIFEKDRALARRFQKIDIVEPSVDDTTRILMGLKSRYEAHHGVRYTVKAIRAAVELSAKYINDRHLPDKAIDVIDEAGAGQRLLPASKRKKVINVQEIEAIVAKIARIPEKSVSSSDKEVLKNLERNLKMVVFGQDKAIEVLTDSIRLSRSGLGNERRPVGCFLFAGPTGVGKTEVTQQLGKALGVEVLRFDMSEYMERHTVSRLIGAPPGYVGFEQGGLLTDSVLKHPHSVVLLDEIEKAHPDVFNLLLQVMDNGTLTDNNGRKADFRNVILVMTTNAGVQETQRKSIGFQQQDMSHDAMAVINKTFSPEFRNRLDHTIWFNHLDMQVIHQVVDKFIVELQVQLDAKGVSLEVSEGVRHWLAEKGYDRAMGARPMGRVIQEHLKKPLANEILFGSLVDGGVVKVELINDSLSFDYQVNVEAVSH

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9509	-	-

Protein sequence: 751 a.a.

						>T6CP012961 CP006870:c2938176-2935924 [Aeromonas hydrophila NJ-35] [TssH]
MLNKDLEKTLNEAFKLARDERHEFMTVEHLLLALLDNSSAHEALSSCGADVELMRKEISAFISQTTPLIPRDDEDRETQP
TLGFQRVLQRAVFHVQSSGNTEVSGANVLVAIFSEQESQAAYFLKKAEISRLDVVNFLSHGVRKDSKPDNSNNNAESDDD
VSAAQIESFATNLNQLVLEGRIDPLIGRDQELNRTIQVLCRRRKNNPLLVGEAGVGKTAIAEGLAYRIVKGDVPEVIAGS
TIYSLDMGSLLAGTKYRGDFEKRLKVLLKQIERQEGAILFIDEIHTIIGAGAASGGQLDAANLIKPLLSNGLLRCVGSTT
YQEYSQIFEKDRALARRFQKIDIVEPSVDDTTRILMGLKSRYEAHHGVRYTVKAIRAAVELSAKYINDRHLPDKAIDVID
EAGAGQRLLPASKRKKVINVQEIEAIVAKIARIPEKSVSSSDKEVLKNLERNLKMVVFGQDKAIEVLTDSIRLSRSGLGN
ERRPVGCFLFAGPTGVGKTEVTQQLGKALGVEVLRFDMSEYMERHTVSRLIGAPPGYVGFEQGGLLTDSVLKHPHSVVLL
DEIEKAHPDVFNLLLQVMDNGTLTDNNGRKADFRNVILVMTTNAGVQETQRKSIGFQQQDMSHDAMAVINKTFSPEFRNR
LDHTIWFNHLDMQVIHQVVDKFIVELQVQLDAKGVSLEVSEGVRHWLAEKGYDRAMGARPMGRVIQEHLKKPLANEILFG
SLVDGGVVKVELINDSLSFDYQVNVEAVSH*					

Nucleotide sequence: 2253 bp

						>T6CP012961 CP006870:c2938176-2935924 [Aeromonas hydrophila NJ-35] [TssH]
ATGTTGAATAAAGATCTAGAAAAGACGCTGAACGAGGCTTTCAAACTGGCCCGTGACGAGCGCCACGAATTCATGACTGT
CGAGCACCTGTTGTTAGCCCTGCTGGATAACAGCTCAGCCCATGAGGCGCTGAGTTCTTGTGGTGCTGACGTGGAGCTGA
TGCGCAAAGAGATCAGCGCATTCATCAGTCAAACAACCCCGCTTATTCCCCGTGATGATGAAGATCGTGAAACCCAGCCC
ACACTGGGCTTCCAGCGGGTGCTGCAACGCGCCGTGTTCCATGTCCAATCATCGGGCAACACGGAAGTCAGTGGTGCCAA
CGTATTAGTTGCCATTTTCAGCGAGCAGGAGTCCCAGGCAGCTTATTTCCTGAAAAAGGCCGAGATCAGCCGGCTGGACG
TGGTCAACTTCCTCTCCCATGGCGTGCGCAAAGACAGCAAGCCGGACAACAGCAACAACAATGCCGAGTCCGACGACGAT
GTGTCTGCCGCCCAGATAGAAAGCTTTGCCACCAACCTCAATCAGCTGGTGCTGGAAGGGCGTATCGATCCGCTCATCGG
ACGGGATCAGGAGCTCAACCGTACCATCCAGGTGCTTTGCCGCCGTCGCAAGAACAACCCCTTGCTGGTCGGTGAGGCCG
GGGTAGGCAAGACTGCCATTGCCGAAGGGCTGGCCTATCGTATCGTCAAGGGCGATGTGCCCGAGGTGATTGCGGGCAGC
ACCATTTATTCCCTGGACATGGGCTCGCTGCTGGCGGGGACCAAATATCGCGGTGATTTCGAGAAGCGTCTCAAGGTGCT
GCTCAAGCAGATCGAGCGCCAGGAAGGGGCGATCCTCTTCATCGACGAGATCCACACCATCATAGGGGCAGGGGCGGCCT
CCGGTGGTCAGCTGGATGCGGCCAACCTCATCAAGCCGCTGCTCTCCAATGGCTTGCTGCGTTGTGTCGGCTCCACCACT
TATCAGGAATACTCCCAGATCTTTGAAAAGGACCGTGCTCTGGCGCGTCGCTTCCAGAAGATCGATATCGTCGAGCCCAG
TGTGGATGACACCACTCGCATCCTGATGGGTCTCAAGAGCCGCTATGAGGCTCACCACGGGGTGCGCTATACCGTCAAGG
CTATCCGCGCCGCGGTGGAGCTCTCTGCCAAGTACATCAATGACCGTCATCTGCCTGACAAGGCGATCGACGTGATCGAC
GAAGCCGGTGCCGGGCAACGGCTGCTGCCGGCGAGCAAGCGCAAGAAAGTGATCAATGTGCAGGAGATCGAAGCCATTGT
GGCCAAGATTGCCCGCATCCCGGAGAAATCAGTCTCCTCTTCCGACAAGGAAGTGCTGAAGAATCTGGAGCGCAACCTCA
AGATGGTGGTATTTGGTCAGGACAAGGCCATCGAGGTGCTGACCGATTCCATTCGATTGTCACGTTCGGGCCTTGGCAAT
GAGCGTCGCCCGGTGGGTTGCTTCCTGTTTGCCGGCCCGACCGGGGTGGGCAAGACCGAGGTCACTCAGCAGTTGGGCAA
GGCGCTCGGCGTCGAGGTACTGCGCTTTGACATGTCGGAGTACATGGAACGCCACACCGTCTCCCGCCTGATCGGGGCAC
CTCCCGGCTATGTGGGCTTTGAGCAGGGTGGACTCCTGACCGACTCGGTGCTCAAGCATCCGCACTCAGTGGTGTTGCTG
GACGAGATCGAAAAAGCGCACCCGGACGTGTTCAACCTGCTGCTGCAGGTGATGGACAACGGCACCCTGACCGACAACAA
CGGGCGCAAGGCGGATTTTCGCAATGTGATCCTGGTGATGACCACCAACGCCGGCGTGCAGGAGACCCAGCGCAAATCCA
TCGGTTTCCAGCAGCAGGATATGAGCCACGATGCCATGGCGGTGATCAACAAGACGTTTAGCCCGGAGTTTCGCAACCGG
CTCGATCACACCATCTGGTTCAACCATCTGGATATGCAGGTGATACATCAGGTGGTCGACAAGTTCATCGTCGAGTTGCA
GGTACAACTGGATGCCAAGGGCGTGTCTCTTGAAGTGTCCGAGGGTGTCCGTCACTGGTTGGCCGAGAAGGGATATGACA
GGGCCATGGGAGCGAGACCCATGGGGCGGGTCATCCAGGAACACCTCAAGAAACCCCTTGCCAATGAGATCCTGTTCGGA
TCCTTGGTCGATGGGGGGGTAGTGAAGGTTGAGTTGATAAATGACAGCCTCAGCTTTGACTATCAGGTCAATGTTGAAGC
TGTTTCCCACTAA					