Detailed information of component protein


Summary

Component ID T6CP012949
Component protein type TssK
T6SS ID (Type) T6SS00929 experimental (Type i3)
Strain Serratia marcescens subsp. marcescens Db11
Replicon chromosome I
Sequence Protein sequence (449 a.a.); Nucleotide sequence (1347 bp)
Reference
[1] Srikannathasan V, English G, Bui NK, et al. Structural basis for type VI secreted peptidoglycan DL-endopeptidase function, specificity and neutralization in Serratia marcescens. Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2468-82. doi: 10.1107/S0907444913022725. Epub 2013 Nov 19. PMID: 24311588
[2] Heymann JB, Bartho JD, Rybakova D, et al. Three-dimensional structure of the toxin-delivery particle antifeeding prophage of Serratia entomophila. J Biol Chem. 2013 Aug 30;288(35):25276-25284. doi: 10.1074/jbc.M113.456145. Epub 2013 Jul 15. PMID: 23857636
[3] Rao VA, Shepherd SM, English G, et al. The structure of Serratia marcescens Lip, a membrane-bound component of the type VI secretion system. Acta Crystallogr D Biol Crystallogr. 2011 Dec;67(Pt 12):1065-72. doi: 10.1107/S0907444911046300. Epub 2011 Nov 18. PMID: 22120744
[4] Murdoch SL, Trunk K, English G, et al. The opportunistic pathogen Serratia marcescens utilizes type VI secretion to target bacterial competitors. J Bacteriol. 2011 Nov;193(21):6057-69. doi: 10.1128/JB.05671-11. Epub 2011 Sep 2. PMID: 21890705
[5] Fritsch MJ, Trunk K, Diniz JA, et al. Proteomic identification of novel secreted antibacterial toxins of the Serratia marcescens type VI secretion system. Mol Cell Proteomics. 2013 Oct;12(10):2735-49. doi: 10.1074/mcp.M113.030502. Epub 2013 Jul 9. PMID: 23842002
[6] English G, Trunk K, Rao VA, et al. New secreted toxins and immunity proteins encoded within the Type VI secretion system gene cluster of Serratia marcescens. Mol Microbiol. 2012 Nov;86(4):921-36. doi: 10.1111/mmi.12028. Epub 2012 Sep 27. PMID: 22957938
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) SMDB11_2253
Coordinate (Strand) 2374478..2375824 (+)
NCBI ID CDG12828.1
GenBank HG326223
Uniprot ID -
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasE PF05936.14 6.8e-150 19..442

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-448 MKDAHKVVWTEGMFLRPHHFQQAENYLEGYMRNWGQAHSGCFWGFLTLDLDQTLLRQGKIALNAASGIMPDGTPFRFSGAQQAPAPLTIAENKTGENVVLALPTYRAGREDVIFQESPEALARYLAYENEVDDLNAVSVGSAALQFGRLRLRLMLESELNAEWTALGVTRVLEKRGDNSLRLDTTQIPPMLNCQGNPVLKTFINDLQGLLQQRSQQMSQRLLQPGRGGSSEMVDFMLLQLINRHLGQVSHAYHLDHLHPERLFADWLQFATELASFSAQRTPEGRLPVYDHDNLALCFGKLMLLLRQGLSVVLEDNAIQLTLVERSHGLNVATVQDAKMMRDFGFVLAVRADVAAEVLLTHFPAQMKIAPVTRIRDLVQLQLPGIGLRTMPAAPRQIPYHAGYTYFELEKGGDLWKQMEKSSAFALHLAGEFPGLDMEFWAIRSHTDR



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9935 - -



  Protein sequence: 449 a.a.    

>T6CP012949 HG326223:2374478-2375824 [Serratia marcescens subsp. marcescens Db11] [TssK]
MKDAHKVVWTEGMFLRPHHFQQAENYLEGYMRNWGQAHSGCFWGFLTLDLDQTLLRQGKIALNAASGIMPDGTPFRFSGA
QQAPAPLTIAENKTGENVVLALPTYRAGREDVIFQESPEALARYLAYENEVDDLNAVSVGSAALQFGRLRLRLMLESELN
AEWTALGVTRVLEKRGDNSLRLDTTQIPPMLNCQGNPVLKTFINDLQGLLQQRSQQMSQRLLQPGRGGSSEMVDFMLLQL
INRHLGQVSHAYHLDHLHPERLFADWLQFATELASFSAQRTPEGRLPVYDHDNLALCFGKLMLLLRQGLSVVLEDNAIQL
TLVERSHGLNVATVQDAKMMRDFGFVLAVRADVAAEVLLTHFPAQMKIAPVTRIRDLVQLQLPGIGLRTMPAAPRQIPYH
AGYTYFELEKGGDLWKQMEKSSAFALHLAGEFPGLDMEFWAIRSHTDR*

  Nucleotide sequence: 1347 bp    

>T6CP012949 HG326223:2374478-2375824 [Serratia marcescens subsp. marcescens Db11] [TssK]
ATGAAAGATGCTCATAAGGTTGTCTGGACTGAGGGGATGTTTTTGCGCCCTCACCATTTCCAGCAGGCGGAAAACTATCT
CGAAGGCTATATGCGCAACTGGGGCCAGGCCCACAGCGGCTGTTTCTGGGGGTTCCTCACCCTGGATCTGGACCAAACGC
TGCTGCGCCAGGGCAAGATTGCGCTCAACGCCGCCAGCGGCATCATGCCGGACGGCACGCCGTTTCGCTTCTCCGGCGCC
CAGCAGGCGCCCGCGCCGCTGACCATCGCTGAAAACAAGACCGGTGAAAACGTGGTGCTGGCTCTGCCGACCTACCGCGC
GGGGCGTGAAGACGTGATCTTTCAGGAAAGCCCGGAGGCGCTGGCGCGCTATCTGGCTTATGAGAACGAAGTCGACGATC
TCAACGCCGTCTCGGTGGGCAGCGCGGCCTTGCAGTTCGGTCGCCTGCGCCTGCGGCTGATGCTGGAGAGTGAGCTCAAC
GCCGAATGGACGGCGCTCGGCGTTACCCGCGTGCTGGAAAAGCGCGGTGACAACAGCCTGCGTCTCGATACCACGCAGAT
CCCGCCGATGCTGAACTGCCAGGGCAACCCGGTACTGAAAACCTTCATCAACGATCTGCAGGGCCTGCTGCAACAGCGCA
GCCAGCAGATGAGCCAGCGCCTGCTGCAGCCGGGCCGCGGCGGCAGTTCCGAAATGGTCGATTTCATGCTGCTGCAGCTG
ATCAACCGCCATCTCGGCCAGGTGAGCCACGCCTACCACCTCGATCATCTGCACCCGGAGCGCCTGTTCGCCGATTGGCT
GCAGTTCGCCACCGAGCTCGCCAGCTTCTCGGCGCAGCGCACCCCGGAAGGCCGCCTGCCGGTGTATGACCACGACAACC
TGGCGCTGTGCTTCGGCAAGCTGATGCTGCTGCTGCGTCAGGGGCTGTCGGTGGTGCTGGAAGACAACGCCATTCAGCTG
ACGCTGGTCGAACGCTCCCACGGGCTGAACGTCGCCACCGTGCAGGACGCCAAAATGATGCGCGATTTTGGCTTCGTTCT
GGCAGTGCGCGCCGACGTGGCCGCCGAGGTGTTGCTGACCCACTTCCCGGCGCAGATGAAGATCGCCCCGGTCACGCGCA
TCCGCGATCTGGTGCAGCTGCAGCTGCCGGGCATCGGTCTGCGCACCATGCCCGCTGCGCCGCGCCAGATCCCCTATCAC
GCCGGCTACACCTACTTCGAGCTGGAAAAAGGCGGCGACCTGTGGAAACAGATGGAGAAATCCAGCGCCTTCGCCCTGCA
CCTGGCCGGCGAATTCCCGGGGCTGGACATGGAATTTTGGGCGATTCGCAGCCATACGGACCGGTAA