SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP012942
Component protein type	TssB
T6SS ID (Type)	T6SS00937 (Type i1)
Strain	Escherichia coli DE719
Replicon	chromosome
Sequence	Protein sequence (168 a.a.); Nucleotide sequence (504 bp)
Reference	[1] Wang S, Dai J, Meng Q, et al. DotU expression is highly induced during in vivo infection and responsible for virulence and Hcp1 secretion in avian pathogenic Escherichia coli. Front Microbiol. 2014 Nov 7;5:588. doi: 10.3389/fmicb.2014.00588. eCollection 2014. PMID: 25426107

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	19609..20112 (-)
NCBI ID	AIU68819.1
GenBank	KM251715
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	1.1e-44	10..161

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-167	MSKMNNNGGSVAPKERISVRYTPKVDGVAADIELPLNLLITGNLKGKPDNTPLDERTAIAINRYNLNAVISEADIEREFVVPAELSDAPNEQMYVNLKVKSMDDLSPDHIASQVPEIKRLLELREALVALKSPLGNIPAFRAQLQALLENEDTREQLIQELGIAVQK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9778	-	-

Protein sequence: 168 a.a.

						>T6CP012942 KM251715:c20112-19609 [Escherichia coli] [TssB]
MSKMNNNGGSVAPKERISVRYTPKVDGVAADIELPLNLLITGNLKGKPDNTPLDERTAIAINRYNLNAVISEADIEREFV
VPAELSDAPNEQMYVNLKVKSMDDLSPDHIASQVPEIKRLLELREALVALKSPLGNIPAFRAQLQALLENEDTREQLIQE
LGIAVQK*					

Nucleotide sequence: 504 bp

						>T6CP012942 KM251715:c20112-19609 [Escherichia coli] [TssB]
ATGAGCAAAATGAACAACAATGGCGGCAGTGTCGCGCCGAAAGAAAGAATCAGCGTTCGGTATACACCAAAAGTTGATGG
GGTGGCTGCTGATATCGAATTGCCGCTGAATTTATTGATCACCGGCAATCTGAAAGGGAAACCAGATAACACGCCGTTGG
ATGAGCGAACTGCCATTGCTATCAACCGCTATAACCTCAACGCAGTCATTTCAGAAGCTGACATTGAACGGGAGTTTGTT
GTTCCGGCTGAACTCAGTGACGCTCCCAATGAGCAAATGTACGTCAACCTGAAAGTCAAATCTATGGATGACCTTTCTCC
TGACCATATTGCCAGCCAGGTACCAGAAATAAAACGTCTGCTCGAATTGCGTGAAGCCCTAGTCGCACTTAAGTCTCCAC
TAGGCAATATTCCAGCCTTCCGCGCACAACTTCAGGCGCTACTGGAAAACGAAGACACCCGCGAGCAGCTGATCCAGGAA
CTGGGTATCGCCGTTCAGAAATAA					