SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP012937
Component protein type	TssG
T6SS ID (Type)	T6SS00937 (Type i1)
Strain	Escherichia coli DE719
Replicon	chromosome
Sequence	Protein sequence (350 a.a.); Nucleotide sequence (1050 bp)
Reference	[1] Wang S, Dai J, Meng Q, et al. DotU expression is highly induced during in vivo infection and responsible for virulence and Hcp1 secretion in avian pathogenic Escherichia coli. Front Microbiol. 2014 Nov 7;5:588. doi: 10.3389/fmicb.2014.00588. eCollection 2014. PMID: 25426107

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	14811..15860 (-)
NCBI ID	700289669
GenBank	KM251715
Uniprot ID	Q6TKU6_ECOLX
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	1.4e-85	23..335

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-349	MAGENGPARPDIATKPPLPADVRNCNFYVLMEALYRRHGAPGQDISLRTEPAREIVRFSSDASISFPGTDLSALSRSQNGQYVLQTRFLGFSGSQSPLPGYYLDQMAQESAQNEDGLKEFLDLFSHRWTQFAYHAWRKYRYYICFRSGGTDTFSQRMYALVGLGNQSVRDRLAINHSKMLAYAGILATPGRAPEVICNLVSHCFDLPDVTIERWQLRKVAIDPAHQNRLGVRNPKGKTAGHIPGRSILGVNFTLGARVPDRSGKFLLQIGGLSRERYLSFLPDGENHLPLTMFLSFILRDQFAWDLRLCLAPQQAKGMRLSDPASSRLGRTSFIGQPKVPPAITIRIRE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9872	-	-

Protein sequence: 350 a.a.

						>T6CP012937 KM251715:c15860-14811 [Escherichia coli] [TssG]
MAGENGPARPDIATKPPLPADVRNCNFYVLMEALYRRHGAPGQDISLRTEPAREIVRFSSDASISFPGTDLSALSRSQNG
QYVLQTRFLGFSGSQSPLPGYYLDQMAQESAQNEDGLKEFLDLFSHRWTQFAYHAWRKYRYYICFRSGGTDTFSQRMYAL
VGLGNQSVRDRLAINHSKMLAYAGILATPGRAPEVICNLVSHCFDLPDVTIERWQLRKVAIDPAHQNRLGVRNPKGKTAG
HIPGRSILGVNFTLGARVPDRSGKFLLQIGGLSRERYLSFLPDGENHLPLTMFLSFILRDQFAWDLRLCLAPQQAKGMRL
SDPASSRLGRTSFIGQPKVPPAITIRIRE*					

Nucleotide sequence: 1050 bp

						>T6CP012937 KM251715:c15860-14811 [Escherichia coli] [TssG]
ATGGCAGGAGAAAATGGGCCAGCACGCCCTGATATAGCGACAAAACCGCCGTTACCGGCGGATGTGCGCAACTGCAATTT
CTATGTGCTGATGGAGGCGCTGTACCGTCGGCATGGCGCGCCGGGCCAGGACATTTCCCTGCGTACCGAGCCTGCCCGGG
AGATCGTGCGTTTCAGCTCTGACGCCAGTATCAGTTTTCCGGGTACGGACCTGAGTGCGCTTTCCCGTAGCCAGAACGGA
CAGTATGTACTGCAAACCCGGTTCCTGGGTTTTTCCGGCAGCCAGTCGCCGCTACCGGGCTATTATCTGGATCAGATGGC
GCAGGAGTCCGCACAGAACGAAGACGGCCTGAAAGAATTTCTCGATCTATTCAGCCACCGCTGGACACAGTTTGCCTATC
ATGCCTGGCGCAAATACCGCTACTACATCTGCTTTCGTAGCGGCGGTACTGACACCTTTTCACAGCGCATGTACGCACTG
GTCGGGCTGGGTAATCAGAGCGTGCGTGACAGGCTTGCCATTAACCACAGCAAAATGCTGGCCTACGCCGGAATTCTGGC
AACACCAGGCCGCGCGCCGGAGGTTATCTGTAATCTGGTGTCGCACTGTTTTGATTTGCCGGATGTCACCATTGAACGCT
GGCAACTGCGTAAAGTCGCCATTGATCCGGCACACCAGAACCGGCTGGGTGTACGTAATCCGAAAGGAAAAACGGCTGGA
CATATTCCGGGACGCTCCATACTCGGCGTTAATTTTACCCTCGGCGCACGGGTGCCGGATCGCAGCGGAAAATTCCTGTT
GCAGATTGGCGGCCTGTCGCGGGAGCGTTATCTCTCATTTTTACCCGATGGCGAAAATCACCTGCCGCTGACCATGTTCC
TTTCCTTTATTCTGCGCGACCAGTTTGCCTGGGATTTACGGCTGTGCCTGGCGCCACAGCAGGCTAAAGGGATGCGCCTT
AGCGATCCGGCCAGCTCCCGCCTCGGCAGAACCAGCTTTATTGGTCAGCCGAAAGTACCGCCCGCCATCACAATCCGAAT
CAGGGAATAA					