SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP012936
Component protein type	TssJ
T6SS ID (Type)	T6SS00937 (Type i1)
Strain	Escherichia coli DE719
Replicon	chromosome
Sequence	Protein sequence (175 a.a.); Nucleotide sequence (525 bp)
Reference	[1] Wang S, Dai J, Meng Q, et al. DotU expression is highly induced during in vivo infection and responsible for virulence and Hcp1 secretion in avian pathogenic Escherichia coli. Front Microbiol. 2014 Nov 7;5:588. doi: 10.3389/fmicb.2014.00588. eCollection 2014. PMID: 25426107

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	12992..13516 (-)
NCBI ID	700289666
GenBank	KM251715
Uniprot ID	Q6TKU4_ECOLX
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	4.7e-23	45..164

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-174	MNGKAFLACVLMSVVLTGCETAKKISQVIRNPDIQVGKLMDQSTELTVTLLTEPDSNLTADGEAAPVDVQLVYLSDDSKFHAADYDQVATTALPDVLGKNYIDHQDFNLLPDTVKTLPPIKLDEKTGYIGVIAYFSDDQATEWKQIESVESIGHHYRLLVHIRASAIEMKKEEN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9991	pos: 18-19. LTG-CE.	MNGKAFLACVLMSVVLTG

Protein sequence: 175 a.a.

						>T6CP012936 KM251715:c13516-12992 [Escherichia coli] [TssJ]
MNGKAFLACVLMSVVLTGCETAKKISQVIRNPDIQVGKLMDQSTELTVTLLTEPDSNLTADGEAAPVDVQLVYLSDDSKF
HAADYDQVATTALPDVLGKNYIDHQDFNLLPDTVKTLPPIKLDEKTGYIGVIAYFSDDQATEWKQIESVESIGHHYRLLV
HIRASAIEMKKEEN*					

Nucleotide sequence: 525 bp

						>T6CP012936 KM251715:c13516-12992 [Escherichia coli] [TssJ]
ATGAACGGCAAAGCGTTTCTGGCCTGCGTTCTGATGAGCGTCGTATTAACTGGCTGTGAAACAGCGAAAAAAATCAGCCA
GGTGATCCGCAATCCGGATATTCAGGTCGGAAAGCTGATGGATCAGTCAACCGAGCTGACCGTCACGCTGCTGACCGAGC
CGGACAGCAACCTGACGGCGGATGGCGAAGCCGCGCCGGTGGATGTCCAGTTGGTTTATCTGAGCGACGACTCAAAATTC
CATGCCGCCGACTACGACCAGGTTGCCACCACCGCGCTGCCCGACGTGCTGGGGAAAAACTATATCGATCACCAGGACTT
CAACCTGTTGCCGGATACCGTAAAAACACTGCCGCCGATCAAGTTGGATGAGAAAACCGGTTATATCGGTGTCATTGCCT
ATTTTTCAGACGACCAGGCCACAGAATGGAAACAAATTGAGTCGGTAGAAAGTATCGGCCACCACTATCGCCTGCTGGTG
CATATCCGCGCCAGTGCGATTGAGATGAAAAAAGAGGAAAACTGA					