SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP012925
Component protein type	TssD
T6SS ID (Type)	T6SS00937 (Type i1)
Strain	Escherichia coli DE719
Replicon	chromosome
Sequence	Protein sequence (160 a.a.); Nucleotide sequence (480 bp)
Reference	[1] Wang S, Dai J, Meng Q, et al. DotU expression is highly induced during in vivo infection and responsible for virulence and Hcp1 secretion in avian pathogenic Escherichia coli. Front Microbiol. 2014 Nov 7;5:588. doi: 10.3389/fmicb.2014.00588. eCollection 2014. PMID: 25426107

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	501..980 (-)
NCBI ID	700289654
GenBank	KM251715
Uniprot ID	Q6TKT6_ECOLX
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_HCP	PF05638.14	4.2e-22	7..143

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-159	MANPVYLTLNGELQGLISSGCSSMPSIGNKAQIAHQDQIMVTSLSHGLSRAQNVNHQELTITKPVDKSSPLLGKAISENECLTCDFVFYRTNRFGINEPYYKLKLANARLSNIGLTVPHTINDSPGQAEESVSFTYESINWEHSVAGTSAYSLWSERIF

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.5814	-	-

Protein sequence: 160 a.a.

						>T6CP012925 KM251715:c980-501 [Escherichia coli] [TssD]
MANPVYLTLNGELQGLISSGCSSMPSIGNKAQIAHQDQIMVTSLSHGLSRAQNVNHQELTITKPVDKSSPLLGKAISENE
CLTCDFVFYRTNRFGINEPYYKLKLANARLSNIGLTVPHTINDSPGQAEESVSFTYESINWEHSVAGTSAYSLWSERIF*					

Nucleotide sequence: 480 bp

						>T6CP012925 KM251715:c980-501 [Escherichia coli] [TssD]
ATGGCAAATCCCGTCTATCTGACACTGAACGGAGAACTTCAGGGGCTGATTTCATCCGGATGCTCGTCAATGCCGTCCAT
CGGTAACAAAGCCCAGATTGCACATCAGGATCAGATCATGGTGACGTCACTGTCCCATGGTCTGAGCCGTGCGCAGAATG
TCAACCATCAGGAGTTGACCATCACGAAACCGGTGGACAAATCCTCCCCTCTGCTGGGCAAGGCCATTTCAGAGAATGAA
TGTCTGACCTGTGATTTTGTATTCTACCGCACCAACCGCTTTGGAATTAATGAACCGTACTACAAGCTGAAACTGGCTAA
CGCCCGGCTCTCAAACATCGGCCTTACCGTTCCCCATACCATTAACGACAGTCCAGGGCAGGCGGAAGAGTCTGTGTCAT
TTACCTATGAGAGCATCAATTGGGAGCACAGCGTCGCCGGAACCAGTGCATACAGCCTTTGGAGTGAACGGATTTTCTAA					