SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP012916
Component protein type	TssJ
T6SS ID (Type)	T6SS00928 (Type i1)
Strain	Campylobacter jejuni 108
Replicon	chromosome
Sequence	Protein sequence (149 a.a.); Nucleotide sequence (447 bp)
Reference	[1] Bleumink-Pluym NM, van Alphen LB, Bouwman LI, et al. Identification of a functional type VI secretion system in Campylobacter jejuni conferring capsule polysaccharide sensitive cytotoxicity. PLoS Pathog. 2013;9(5):e1003393. doi: 10.1371/journal.ppat.1003393. Epub 2013 May 30. PMID: 23737749

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	7489..7935 (-)
NCBI ID	510027007
GenBank	JX436460
Uniprot ID	R9TJM7_CAMJU
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	9.6e-14	23..126

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-148	MFCKKSLFFLLPLFFCACSSVVSVKINNVENSNLNNRHDDVPVTAIVYQLKDIKKFEEASDIDLATREEGVLGKDKLDSIKTQIAPKDNIIAVKVDEEEVPYVGILVLFANNTKKTTKIWAKTEDANGFGKNKYLKFEISKEGIKRIK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9948	pos: 17-18. FCA-CS.	MFCKKSLFFLLPLFFCA

Protein sequence: 149 a.a.

						>T6CP012916 JX436460:c7935-7489 [Campylobacter jejuni] [TssJ]
MFCKKSLFFLLPLFFCACSSVVSVKINNVENSNLNNRHDDVPVTAIVYQLKDIKKFEEASDIDLATREEGVLGKDKLDSI
KTQIAPKDNIIAVKVDEEEVPYVGILVLFANNTKKTTKIWAKTEDANGFGKNKYLKFEISKEGIKRIK*					

Nucleotide sequence: 447 bp

						>T6CP012916 JX436460:c7935-7489 [Campylobacter jejuni] [TssJ]
ATGTTTTGCAAAAAAAGCTTATTTTTTTTATTGCCATTATTCTTTTGCGCTTGCTCAAGTGTTGTTAGTGTTAAGATAAA
TAATGTAGAGAATTCCAATCTTAATAATAGACATGATGATGTTCCTGTTACGGCTATTGTATATCAACTTAAGGATATTA
AAAAATTTGAAGAAGCAAGTGATATAGATTTGGCTACTAGAGAAGAGGGGGTGCTAGGCAAGGATAAACTTGATTCTATT
AAAACGCAAATTGCACCTAAAGACAATATTATCGCGGTTAAAGTAGATGAAGAAGAGGTTCCTTATGTAGGCATTCTTGT
ATTATTTGCAAATAATACAAAAAAAACAACAAAAATATGGGCAAAGACCGAAGATGCAAATGGGTTTGGGAAGAATAAGT
ATTTAAAATTTGAAATATCCAAAGAAGGTATCAAAAGAATTAAATAG					