Detailed information of component protein
Summary
| Component ID | T6CP012909 |
| Component protein type | TagJ |
| T6SS ID (Type) | T6SS00897  (Type i5) |
| Strain | Rhizobium leguminosarum bv. trifolii |
| Replicon | chromosome |
| Sequence | Protein sequence (277 a.a.); Nucleotide sequence (831 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | - |
| Coordinate (Strand) | 5466..6296 (+) |
| NCBI ID | 16326481 |
| GenBank | AF361470 |
| Uniprot ID | Q93EC9_RHILT |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| ImpE | PF07024.15 | 2.6e-33 | 142..261 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-276 | MTLSASIAQSLSDNALDDAIEEVKAHLKVKPSDQEARHLYIDLLVLAGDYQRADNQCSLAATLSPDATMGFALLRNELRAMAARDAWFTSGALPEFPQGPSELDKLAVRLGIAHRDGNADEARTTLDALEKLRGERPLIWNGKAVSDFRDLDDRTPHALEVIMTGGGYLWIDFAKIAALSIEPIARPRDLAFRRAELSLIDGAAASVLLPAVYHGTGKDETLRLGRETEWIEETTGITSGRGQRCYLAGDEMVSFHDTQSLEIVPAAAAGRQVAHG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9248 | - | - |
Protein sequence: 277 a.a.
>T6CP012909 AF361470:5466-6296 [Rhizobium leguminosarum bv. trifolii] [TagJ]
MTLSASIAQSLSDNALDDAIEEVKAHLKVKPSDQEARHLYIDLLVLAGDYQRADNQCSLAATLSPDATMGFALLRNELRA
MAARDAWFTSGALPEFPQGPSELDKLAVRLGIAHRDGNADEARTTLDALEKLRGERPLIWNGKAVSDFRDLDDRTPHALE
VIMTGGGYLWIDFAKIAALSIEPIARPRDLAFRRAELSLIDGAAASVLLPAVYHGTGKDETLRLGRETEWIEETTGITSG
RGQRCYLAGDEMVSFHDTQSLEIVPAAAAGRQVAHG*
MTLSASIAQSLSDNALDDAIEEVKAHLKVKPSDQEARHLYIDLLVLAGDYQRADNQCSLAATLSPDATMGFALLRNELRA
MAARDAWFTSGALPEFPQGPSELDKLAVRLGIAHRDGNADEARTTLDALEKLRGERPLIWNGKAVSDFRDLDDRTPHALE
VIMTGGGYLWIDFAKIAALSIEPIARPRDLAFRRAELSLIDGAAASVLLPAVYHGTGKDETLRLGRETEWIEETTGITSG
RGQRCYLAGDEMVSFHDTQSLEIVPAAAAGRQVAHG*
Nucleotide sequence: 831 bp
>T6CP012909 AF361470:5466-6296 [Rhizobium leguminosarum bv. trifolii] [TagJ]
ATGACGCTTTCCGCCAGCATCGCCCAATCGCTCAGCGACAATGCCCTCGATGACGCGATCGAGGAGGTCAAGGCGCATCT
GAAGGTCAAGCCCTCGGATCAGGAGGCGCGGCATCTCTATATCGATCTTCTCGTGCTTGCCGGCGATTACCAGCGGGCGG
ACAATCAGTGCAGCCTTGCTGCCACCCTCTCACCCGACGCGACGATGGGTTTCGCCTTGCTGCGCAACGAGCTTCGGGCG
ATGGCGGCGCGCGACGCCTGGTTTACGAGCGGCGCCCTGCCGGAATTTCCGCAGGGGCCGAGCGAGCTCGACAAGCTCGC
CGTCCGCCTCGGTATCGCCCATCGCGACGGCAATGCGGATGAGGCGAGGACGACGCTCGATGCGCTCGAGAAGCTGAGGG
GCGAACGTCCGTTGATCTGGAACGGCAAGGCCGTCTCCGATTTCCGCGACCTCGACGACCGGACACCGCACGCTCTCGAA
GTCATCATGACCGGCGGCGGCTATCTCTGGATCGATTTTGCCAAGATCGCAGCGCTCTCGATAGAGCCGATTGCCCGGCC
GCGCGATCTGGCCTTCCGCCGCGCCGAGCTCTCCCTGATCGACGGCGCAGCCGCGTCGGTGCTTCTGCCTGCGGTCTATC
ATGGCACCGGCAAGGATGAGACGCTCAGGCTTGGCCGCGAGACCGAGTGGATCGAGGAGACGACCGGCATCACCAGCGGC
CGTGGCCAGCGCTGCTACCTCGCCGGCGACGAAATGGTTTCCTTCCATGACACCCAAAGCCTGGAGATCGTGCCGGCAGC
GGCTGCCGGCAGGCAGGTTGCGCATGGTTGA
ATGACGCTTTCCGCCAGCATCGCCCAATCGCTCAGCGACAATGCCCTCGATGACGCGATCGAGGAGGTCAAGGCGCATCT
GAAGGTCAAGCCCTCGGATCAGGAGGCGCGGCATCTCTATATCGATCTTCTCGTGCTTGCCGGCGATTACCAGCGGGCGG
ACAATCAGTGCAGCCTTGCTGCCACCCTCTCACCCGACGCGACGATGGGTTTCGCCTTGCTGCGCAACGAGCTTCGGGCG
ATGGCGGCGCGCGACGCCTGGTTTACGAGCGGCGCCCTGCCGGAATTTCCGCAGGGGCCGAGCGAGCTCGACAAGCTCGC
CGTCCGCCTCGGTATCGCCCATCGCGACGGCAATGCGGATGAGGCGAGGACGACGCTCGATGCGCTCGAGAAGCTGAGGG
GCGAACGTCCGTTGATCTGGAACGGCAAGGCCGTCTCCGATTTCCGCGACCTCGACGACCGGACACCGCACGCTCTCGAA
GTCATCATGACCGGCGGCGGCTATCTCTGGATCGATTTTGCCAAGATCGCAGCGCTCTCGATAGAGCCGATTGCCCGGCC
GCGCGATCTGGCCTTCCGCCGCGCCGAGCTCTCCCTGATCGACGGCGCAGCCGCGTCGGTGCTTCTGCCTGCGGTCTATC
ATGGCACCGGCAAGGATGAGACGCTCAGGCTTGGCCGCGAGACCGAGTGGATCGAGGAGACGACCGGCATCACCAGCGGC
CGTGGCCAGCGCTGCTACCTCGCCGGCGACGAAATGGTTTCCTTCCATGACACCCAAAGCCTGGAGATCGTGCCGGCAGC
GGCTGCCGGCAGGCAGGTTGCGCATGGTTGA