Detailed information of component protein
Summary
| Component ID | T6CP012805 |
| Component protein type | TagJ |
| T6SS ID (Type) | T6SS00443  (Type i3) |
| Strain | Methylomonas methanica MC09 |
| Replicon | chromosome |
| Sequence | Protein sequence (270 a.a.); Nucleotide sequence (810 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | METME_RS04320 |
| Coordinate (Strand) | 919535..920344 (+) |
| NCBI ID | WP_013817560.1 |
| RefSeq | NC_015572 |
| Uniprot ID | G0A6J1_METMM |
| KEGG ID | mmt:Metme_0854 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| ImpE | PF07024.15 | 8e-49 | 141..261 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-269 | MISSAAEISLKDGRLDDALAQLQDQVRKNPDVAKHRVFLFQLLALNGQWDRALNQLNVCGNLDNANLAMVQTYREAIRCELLREKIFKGETSPLVFGEPEQWIALLIEACKLSAQSNFTQAGELRDQAFELAPTSSGSFNGEAFEWIADSDSRLGPVTEAIVNGRYYWIPFNRIAKIEIEAPADLRDLVWLPAQFTWTNGGSAFGLIPSRYPGSEKAEDAVLRLARKTEWREPAAGHYFGLGQRILITDNGEHALLDTRELIFNTAAAE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9904 | - | - |
Protein sequence: 270 a.a.
>T6CP012805 NC_015572:919535-920344 [Methylomonas methanica MC09] [TagJ]
MISSAAEISLKDGRLDDALAQLQDQVRKNPDVAKHRVFLFQLLALNGQWDRALNQLNVCGNLDNANLAMVQTYREAIRCE
LLREKIFKGETSPLVFGEPEQWIALLIEACKLSAQSNFTQAGELRDQAFELAPTSSGSFNGEAFEWIADSDSRLGPVTEA
IVNGRYYWIPFNRIAKIEIEAPADLRDLVWLPAQFTWTNGGSAFGLIPSRYPGSEKAEDAVLRLARKTEWREPAAGHYFG
LGQRILITDNGEHALLDTRELIFNTAAAE*
MISSAAEISLKDGRLDDALAQLQDQVRKNPDVAKHRVFLFQLLALNGQWDRALNQLNVCGNLDNANLAMVQTYREAIRCE
LLREKIFKGETSPLVFGEPEQWIALLIEACKLSAQSNFTQAGELRDQAFELAPTSSGSFNGEAFEWIADSDSRLGPVTEA
IVNGRYYWIPFNRIAKIEIEAPADLRDLVWLPAQFTWTNGGSAFGLIPSRYPGSEKAEDAVLRLARKTEWREPAAGHYFG
LGQRILITDNGEHALLDTRELIFNTAAAE*
Nucleotide sequence: 810 bp
>T6CP012805 NC_015572:919535-920344 [Methylomonas methanica MC09] [TagJ]
ATGATTTCAAGTGCTGCGGAAATTAGCTTAAAAGACGGACGCCTGGACGATGCGCTGGCGCAATTACAGGATCAGGTGCG
TAAAAATCCGGATGTGGCTAAGCATCGGGTGTTTTTGTTTCAGCTACTGGCACTTAACGGACAGTGGGATCGAGCGCTCA
ATCAGCTCAACGTCTGCGGTAATTTGGACAATGCGAATTTGGCGATGGTGCAAACCTACCGGGAAGCCATTCGTTGCGAA
TTACTACGCGAGAAAATTTTTAAGGGTGAAACGTCGCCTTTGGTATTCGGCGAGCCCGAACAATGGATTGCATTACTAAT
CGAAGCGTGCAAGTTGTCGGCGCAATCTAACTTTACCCAAGCGGGTGAATTGCGTGATCAGGCTTTCGAGTTGGCGCCGA
CTTCGTCCGGCAGTTTTAATGGCGAGGCGTTCGAATGGATTGCTGATTCGGATAGTCGGCTTGGACCGGTTACGGAAGCG
ATTGTCAATGGTCGTTATTATTGGATTCCCTTTAATCGGATCGCCAAAATAGAGATTGAAGCACCGGCCGATCTGCGGGA
TTTGGTCTGGCTGCCGGCCCAATTTACCTGGACCAACGGCGGCAGCGCATTTGGTTTAATTCCATCCCGCTATCCGGGTA
GCGAAAAAGCCGAGGATGCCGTGCTGCGTTTGGCGCGCAAAACGGAATGGCGGGAGCCCGCGGCCGGCCATTACTTTGGT
TTGGGGCAGCGCATTTTGATAACCGACAATGGCGAACATGCTTTATTGGATACTCGGGAATTAATCTTTAATACCGCTGC
TGCCGAATAA
ATGATTTCAAGTGCTGCGGAAATTAGCTTAAAAGACGGACGCCTGGACGATGCGCTGGCGCAATTACAGGATCAGGTGCG
TAAAAATCCGGATGTGGCTAAGCATCGGGTGTTTTTGTTTCAGCTACTGGCACTTAACGGACAGTGGGATCGAGCGCTCA
ATCAGCTCAACGTCTGCGGTAATTTGGACAATGCGAATTTGGCGATGGTGCAAACCTACCGGGAAGCCATTCGTTGCGAA
TTACTACGCGAGAAAATTTTTAAGGGTGAAACGTCGCCTTTGGTATTCGGCGAGCCCGAACAATGGATTGCATTACTAAT
CGAAGCGTGCAAGTTGTCGGCGCAATCTAACTTTACCCAAGCGGGTGAATTGCGTGATCAGGCTTTCGAGTTGGCGCCGA
CTTCGTCCGGCAGTTTTAATGGCGAGGCGTTCGAATGGATTGCTGATTCGGATAGTCGGCTTGGACCGGTTACGGAAGCG
ATTGTCAATGGTCGTTATTATTGGATTCCCTTTAATCGGATCGCCAAAATAGAGATTGAAGCACCGGCCGATCTGCGGGA
TTTGGTCTGGCTGCCGGCCCAATTTACCTGGACCAACGGCGGCAGCGCATTTGGTTTAATTCCATCCCGCTATCCGGGTA
GCGAAAAAGCCGAGGATGCCGTGCTGCGTTTGGCGCGCAAAACGGAATGGCGGGAGCCCGCGGCCGGCCATTACTTTGGT
TTGGGGCAGCGCATTTTGATAACCGACAATGGCGAACATGCTTTATTGGATACTCGGGAATTAATCTTTAATACCGCTGC
TGCCGAATAA