SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP012767
Component protein type	TagJ
T6SS ID (Type)	T6SS00301 (Type i3)
Strain	Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853
Replicon	chromosome
Sequence	Protein sequence (275 a.a.); Nucleotide sequence (825 bp)
Reference	[1] Blondel CJ, Jiménez JC, Contreras I, et al. Comparative genomic analysis uncovers 3 novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics. 2009 Aug 4;10:354. doi: 10.1186/1471-2164-10-354. PMID: 19653904 [2] Pezoa D, Blondel CJ, Silva CA, et al. Only one of the two type VI secretion systems encoded in the Salmonella enterica serotype Dublin genome is involved in colonization of the avian and murine hosts. Vet Res. 2014 Jan 9;45:2. doi: 10.1186/1297-9716-45-2. PMID: 24405577

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	SED_RS01810
Coordinate (Strand)	309903..310727 (-)
NCBI ID	446673202
RefSeq	NC_011205
Uniprot ID	M7RXX7_SALDU, A0A0W4S1G5_SALER, A0A2T6X0I2_SALET
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpE	PF07024.15	3.5e-43	145..265

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-274	MKKTDTLPATLSALLQEYSIAEGIQMAEQQVRENPAKALCRHSLFQLLCVAGDWSRALHQLQLCARMEANYTQEARLYRELVRCEMFRHTVFQGEQRPGFLLPQPVWVESLLAALACHDDTGEVDKHRNTALEAITDTGGQWNGGAFDWASDSDSRLGPVLELVTGGVYIWLPFSQIRSLESPQPTRLTDLLWKPVNITLVNGDTHGAWLFTRYSGSESASDALRLCRETAWQDGPGETTVRALGQKVWLTSHGDISLLDMAHCTFHAQENDGA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8434	-	-

Protein sequence: 275 a.a.

						>T6CP012767 NC_011205:c310727-309903 [Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853] [TagJ]
MKKTDTLPATLSALLQEYSIAEGIQMAEQQVRENPAKALCRHSLFQLLCVAGDWSRALHQLQLCARMEANYTQEARLYRE
LVRCEMFRHTVFQGEQRPGFLLPQPVWVESLLAALACHDDTGEVDKHRNTALEAITDTGGQWNGGAFDWASDSDSRLGPV
LELVTGGVYIWLPFSQIRSLESPQPTRLTDLLWKPVNITLVNGDTHGAWLFTRYSGSESASDALRLCRETAWQDGPGETT
VRALGQKVWLTSHGDISLLDMAHCTFHAQENDGA*					

Nucleotide sequence: 825 bp

						>T6CP012767 NC_011205:c310727-309903 [Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853] [TagJ]
ATGAAAAAAACTGACACGCTTCCTGCTACCCTGTCCGCGCTGCTACAGGAATACTCTATTGCGGAGGGGATTCAGATGGC
GGAGCAGCAGGTTCGGGAAAATCCGGCGAAGGCGCTCTGCCGCCACAGCCTGTTTCAGTTGCTGTGTGTGGCCGGAGACT
GGTCGCGGGCGCTACACCAGCTTCAGCTATGTGCCAGGATGGAGGCCAATTATACCCAGGAGGCCCGGCTGTACCGGGAG
CTGGTTCGCTGCGAAATGTTCCGCCACACCGTTTTTCAGGGTGAACAACGACCCGGTTTTCTTCTGCCGCAGCCCGTTTG
GGTGGAGTCACTGCTGGCGGCACTCGCCTGTCATGACGATACCGGTGAGGTTGATAAACACCGTAATACCGCACTGGAAG
CCATAACAGACACGGGCGGGCAGTGGAACGGCGGCGCATTTGACTGGGCGAGCGACAGCGATTCCCGCCTTGGCCCGGTA
CTTGAGCTAGTCACCGGCGGCGTGTATATCTGGCTGCCGTTTTCGCAAATACGCTCGCTGGAATCGCCGCAACCGACGCG
CCTGACCGATCTGCTGTGGAAACCCGTTAATATCACCCTGGTGAATGGCGATACGCACGGCGCCTGGCTCTTTACCCGTT
ACAGCGGTTCGGAAAGTGCATCCGACGCCCTGCGTCTGTGCCGGGAAACCGCCTGGCAGGACGGCCCCGGCGAAACCACC
GTGCGAGCGCTGGGGCAGAAAGTGTGGCTGACCAGCCACGGCGATATCAGCCTGCTGGACATGGCCCATTGTACCTTTCA
TGCTCAGGAAAACGATGGCGCATGA					