Detailed information of component protein


Summary

Component ID T6CP012758
Component protein type TagJ
T6SS ID (Type) T6SS00275 experimental (Type i3)
Strain Paraburkholderia phymatum STM815
Replicon plasmid pBPHY01
Sequence Protein sequence (289 a.a.); Nucleotide sequence (867 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPHY_RS30535
Coordinate (Strand) 669629..670495 (+)
NCBI ID WP_012405333.1
RefSeq NC_010625
Uniprot ID B2JW47_PARP8
KEGG ID bph:Bphy_6117
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ImpE PF07024.15 4.9e-44 135..260
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-288 MTMSRVSLATRLDDVQGMIRREPTAAGHRWTLFQLLCVTQQWERAVQQLQVFAQLNPQQVQAAQAYRDLIRAERWRTKVLDGLAQPGFVIGPPSWVENLAEALRLTAAGQSDAADAIRESALDQAPLVRGGGANHSFEWIADSDSRLGPVCEIITAGRYRWLPFSDIAAWEIGRPATMVDFVWAPCLLTLVDGATVRGFMPARFPSVADATYDSAVLDALRLGNMTIWTDAGLTGVLAEGRKTWATSAGDVGLFELDVCTFGCDADRGSVDSVDGMAMQDEGGVDGAA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9654 - -


  Protein sequence: 289 a.a.    

>T6CP012758 NC_010625:669629-670495 [Paraburkholderia phymatum STM815] [TagJ]
MTMSRVSLATRLDDVQGMIRREPTAAGHRWTLFQLLCVTQQWERAVQQLQVFAQLNPQQVQAAQAYRDLIRAERWRTKVL
DGLAQPGFVIGPPSWVENLAEALRLTAAGQSDAADAIRESALDQAPLVRGGGANHSFEWIADSDSRLGPVCEIITAGRYR
WLPFSDIAAWEIGRPATMVDFVWAPCLLTLVDGATVRGFMPARFPSVADATYDSAVLDALRLGNMTIWTDAGLTGVLAEG
RKTWATSAGDVGLFELDVCTFGCDADRGSVDSVDGMAMQDEGGVDGAA*

  Nucleotide sequence: 867 bp    

>T6CP012758 NC_010625:669629-670495 [Paraburkholderia phymatum STM815] [TagJ]
ATGACGATGTCGCGCGTATCGCTCGCCACACGCCTGGATGACGTGCAGGGCATGATTCGCCGGGAACCCACCGCTGCAGG
TCACCGCTGGACCTTGTTCCAGCTGCTCTGCGTGACGCAGCAGTGGGAGCGGGCGGTCCAGCAACTGCAGGTTTTCGCGC
AATTGAATCCCCAGCAGGTGCAGGCTGCACAGGCGTACCGCGACCTGATCCGCGCCGAACGGTGGCGCACGAAGGTCCTG
GATGGACTTGCGCAGCCTGGTTTTGTTATCGGTCCCCCATCGTGGGTCGAAAATCTCGCTGAGGCGCTGCGTCTGACGGC
GGCTGGGCAAAGTGATGCCGCCGACGCCATTCGTGAATCAGCGCTGGATCAAGCGCCGCTCGTGCGCGGCGGCGGAGCAA
ATCATTCGTTTGAGTGGATCGCCGACAGCGATTCACGTCTCGGCCCTGTCTGCGAAATCATCACGGCAGGACGCTACCGC
TGGCTGCCTTTTTCCGATATTGCGGCTTGGGAAATCGGGCGTCCGGCGACGATGGTTGATTTCGTCTGGGCACCGTGCCT
GCTCACGCTCGTCGACGGTGCCACGGTTCGTGGGTTTATGCCCGCGCGTTTCCCGTCCGTCGCGGACGCGACGTACGACA
GCGCCGTTCTCGATGCGCTGCGCCTCGGCAATATGACGATCTGGACGGATGCGGGCCTCACCGGTGTGCTCGCGGAAGGC
CGCAAGACGTGGGCAACCAGTGCCGGCGATGTGGGACTGTTCGAGCTGGATGTCTGTACGTTTGGCTGTGACGCGGACCG
TGGGTCGGTCGACAGCGTTGACGGGATGGCAATGCAGGATGAAGGGGGCGTCGATGGAGCGGCGTAG