SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP012710
Component protein type	TagJ
T6SS ID (Type)	T6SS00071 (Type i3)
Strain	Burkholderia mallei ATCC 23344
Replicon	chromosome II
Sequence	Protein sequence (322 a.a.); Nucleotide sequence (966 bp)
Reference	[1] Schell MA, Ulrich RL, Ribot WJ, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007 Jun;64(6):1466-85. PMID: 17555434

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BMA_RS25055
Coordinate (Strand)	2088221..2089186 (-)
NCBI ID	WP_004187921.1
RefSeq	NC_006349
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpE	PF07024.15	1.2e-48	149..293

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-321	MTTPAFPAPPGARTSARPHDTPLDARLAAAQAAVRARPVDAASRWALFQLLCVTGQGERAVGQLQAYARFAAQHAHIAHAYRDLIRAERFHARVMAGQARPGFVFDAPPWIDDLLEALRAQAAGARDEADRARSRALDRAPFVAGRGPDAPFDWIADSDSRFGPVCEVVTAGHDRWLPFSDLAGWRLARPGVLLALVWAPCVLTLADGSVAHGFMPARYPGSELADGGGAPGASAAAHEADAHESAAREADALRLGSRTAWRDVGRTGVFALGRKTWSTSAGDAGLFELHACEFGARARAGAVLGGAAREARGGEAADGCA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8430	-	-

Protein sequence: 322 a.a.

						>T6CP012710 NC_006349:c2089186-2088221 [Burkholderia mallei ATCC 23344] [TagJ]
MTTPAFPAPPGARTSARPHDTPLDARLAAAQAAVRARPVDAASRWALFQLLCVTGQGERAVGQLQAYARFAAQHAHIAHA
YRDLIRAERFHARVMAGQARPGFVFDAPPWIDDLLEALRAQAAGARDEADRARSRALDRAPFVAGRGPDAPFDWIADSDS
RFGPVCEVVTAGHDRWLPFSDLAGWRLARPGVLLALVWAPCVLTLADGSVAHGFMPARYPGSELADGGGAPGASAAAHEA
DAHESAAREADALRLGSRTAWRDVGRTGVFALGRKTWSTSAGDAGLFELHACEFGARARAGAVLGGAAREARGGEAADGC
A*					

Nucleotide sequence: 966 bp

						>T6CP012710 NC_006349:c2089186-2088221 [Burkholderia mallei ATCC 23344] [TagJ]
ATGACGACGCCTGCCTTCCCGGCGCCGCCCGGCGCACGCACGTCGGCCCGGCCGCACGATACGCCGCTCGACGCGCGGCT
CGCGGCTGCGCAAGCCGCGGTGCGCGCCCGGCCCGTCGATGCGGCGAGCCGTTGGGCGCTGTTTCAACTGCTCTGCGTGA
CGGGCCAGGGGGAACGCGCCGTCGGGCAGTTGCAGGCTTATGCGCGGTTCGCCGCGCAGCACGCGCACATCGCGCACGCC
TATCGCGACCTGATTCGCGCCGAGCGCTTTCACGCCCGCGTGATGGCGGGCCAGGCGCGGCCGGGATTCGTATTCGATGC
GCCGCCGTGGATCGACGATCTGCTGGAAGCGTTGCGCGCGCAGGCGGCCGGCGCGCGCGACGAAGCCGACCGCGCGCGTT
CGCGCGCGCTCGATCGCGCGCCGTTCGTCGCGGGGCGCGGGCCGGATGCGCCGTTCGACTGGATCGCCGACAGCGATTCG
CGATTCGGGCCGGTCTGCGAAGTCGTGACCGCCGGGCATGATCGTTGGCTGCCGTTTTCGGATCTGGCCGGATGGCGGCT
CGCGCGCCCGGGCGTGCTGCTCGCTCTGGTCTGGGCGCCGTGCGTGCTCACGCTTGCCGACGGCAGTGTCGCGCACGGCT
TCATGCCAGCGCGTTACCCGGGCTCCGAACTGGCCGACGGGGGCGGCGCGCCGGGCGCCTCAGCGGCCGCGCACGAGGCC
GATGCGCACGAGAGCGCCGCGCGCGAGGCCGATGCGTTGCGGCTCGGCAGCCGGACCGCATGGCGCGACGTCGGCCGCAC
CGGCGTGTTCGCGCTCGGCCGCAAGACCTGGTCGACGAGCGCGGGCGACGCCGGCCTTTTCGAGCTGCACGCGTGTGAAT
TCGGCGCGCGCGCCCGTGCGGGCGCCGTGCTCGGCGGCGCGGCGCGCGAAGCGCGCGGCGGGGAGGCGGCCGATGGATGC
GCATGA					