Detailed information of component protein
Summary
| Component ID | T6CP012594 |
| Component protein type | TagF |
| T6SS ID (Type) | T6SS00764  (Type i3) |
| Strain | Pseudomonas protegens CHA0 |
| Replicon | chromosome |
| Sequence | Protein sequence (218 a.a.); Nucleotide sequence (654 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | PFLCHA0_RS30115 |
| Coordinate (Strand) | 6697331..6697984 (-) |
| NCBI ID | WP_011064248.1 |
| RefSeq | NC_021237 |
| Uniprot ID | A0A2C9EVR0_PSEPH, A0A2T0Z764_9PSED, Q4K3P5_PSEF5, A0A7U9Q6M2_PSEFL |
| KEGG ID | pprc:PFLCHA0_c60390, pfl:PFL_6080 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| TagF_N | PF09867.11 | 3.7e-48 | 5..139 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-217 | MTAPGLYGKLASRGDFVSRGLPQSFIGPWDSWLAAGLLASQNALGEQWLNAYLISPLWRFALAPGVCGPQAAVGVVMPSIDRVGRYFPLTVAALLEPEADLASLVGGPEQWFEQVEELLLATLEEGASFEALEEGLQALGAPSPASRIEGSHYAGLQRFYVSDPQARGAVLAAQACAGSSAWWGRGSQYISPMLLRCQGLPPASEFAQFLLGQEGAV |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9421 | - | - |
Protein sequence: 218 a.a.
>T6CP012594 NC_021237:c6697984-6697331 [Pseudomonas protegens CHA0] [TagF]
MTAPGLYGKLASRGDFVSRGLPQSFIGPWDSWLAAGLLASQNALGEQWLNAYLISPLWRFALAPGVCGPQAAVGVVMPSI
DRVGRYFPLTVAALLEPEADLASLVGGPEQWFEQVEELLLATLEEGASFEALEEGLQALGAPSPASRIEGSHYAGLQRFY
VSDPQARGAVLAAQACAGSSAWWGRGSQYISPMLLRCQGLPPASEFAQFLLGQEGAV*
MTAPGLYGKLASRGDFVSRGLPQSFIGPWDSWLAAGLLASQNALGEQWLNAYLISPLWRFALAPGVCGPQAAVGVVMPSI
DRVGRYFPLTVAALLEPEADLASLVGGPEQWFEQVEELLLATLEEGASFEALEEGLQALGAPSPASRIEGSHYAGLQRFY
VSDPQARGAVLAAQACAGSSAWWGRGSQYISPMLLRCQGLPPASEFAQFLLGQEGAV*
Nucleotide sequence: 654 bp
>T6CP012594 NC_021237:c6697984-6697331 [Pseudomonas protegens CHA0] [TagF]
ATGACGGCGCCGGGCCTGTACGGCAAGTTGGCCAGCCGCGGCGATTTTGTCAGCCGTGGCCTGCCGCAGAGTTTCATCGG
CCCCTGGGACAGTTGGCTGGCGGCGGGGCTGCTGGCCAGCCAGAACGCCCTGGGTGAGCAGTGGCTGAACGCCTACCTGA
TCAGCCCGTTGTGGCGCTTCGCCCTGGCCCCCGGGGTCTGCGGGCCCCAGGCGGCGGTCGGGGTGGTGATGCCGAGCATC
GACCGGGTGGGGCGCTATTTCCCCCTGACCGTGGCTGCGCTGCTGGAGCCCGAGGCTGACCTGGCTTCGTTGGTCGGCGG
CCCGGAGCAGTGGTTCGAACAGGTGGAAGAGCTGTTGCTGGCGACCCTGGAAGAGGGCGCCAGTTTCGAAGCCCTGGAAG
AGGGCTTGCAGGCCCTGGGCGCGCCAAGCCCGGCCAGCCGGATCGAAGGCAGCCACTACGCCGGCCTGCAGCGGTTTTAC
GTCAGCGATCCACAGGCCCGGGGCGCGGTACTCGCGGCCCAGGCCTGCGCGGGATCGAGTGCCTGGTGGGGGCGCGGTTC
GCAGTACATCAGCCCAATGCTGTTGCGCTGCCAGGGCTTGCCGCCGGCCAGTGAATTTGCGCAATTTTTGCTCGGACAAG
AGGGTGCCGTCTAA
ATGACGGCGCCGGGCCTGTACGGCAAGTTGGCCAGCCGCGGCGATTTTGTCAGCCGTGGCCTGCCGCAGAGTTTCATCGG
CCCCTGGGACAGTTGGCTGGCGGCGGGGCTGCTGGCCAGCCAGAACGCCCTGGGTGAGCAGTGGCTGAACGCCTACCTGA
TCAGCCCGTTGTGGCGCTTCGCCCTGGCCCCCGGGGTCTGCGGGCCCCAGGCGGCGGTCGGGGTGGTGATGCCGAGCATC
GACCGGGTGGGGCGCTATTTCCCCCTGACCGTGGCTGCGCTGCTGGAGCCCGAGGCTGACCTGGCTTCGTTGGTCGGCGG
CCCGGAGCAGTGGTTCGAACAGGTGGAAGAGCTGTTGCTGGCGACCCTGGAAGAGGGCGCCAGTTTCGAAGCCCTGGAAG
AGGGCTTGCAGGCCCTGGGCGCGCCAAGCCCGGCCAGCCGGATCGAAGGCAGCCACTACGCCGGCCTGCAGCGGTTTTAC
GTCAGCGATCCACAGGCCCGGGGCGCGGTACTCGCGGCCCAGGCCTGCGCGGGATCGAGTGCCTGGTGGGGGCGCGGTTC
GCAGTACATCAGCCCAATGCTGTTGCGCTGCCAGGGCTTGCCGCCGGCCAGTGAATTTGCGCAATTTTTGCTCGGACAAG
AGGGTGCCGTCTAA