SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP012537
Component protein type	Sfa2
T6SS ID (Type)	T6SS00613 (Type i1)
Strain	Pseudomonas aeruginosa M18
Replicon	chromosome
Sequence	Protein sequence (504 a.a.); Nucleotide sequence (1512 bp)
Reference	[1] Wei X, Huang X, Tang L, et al. Global control of GacA in secondary metabolism, primary metabolism, secretion systems, and motility in the rhizobacterium Pseudomonas aeruginosa M18. J Bacteriol. 2013 Aug;195(15):3387-400. doi: 10.1128/JB.00214-13. Epub 2013 May 24. PMID: 23708134

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PAM18_RS17345
Coordinate (Strand)	3764979..3766490 (-)
NCBI ID	WP_003097799.1
RefSeq	NC_017548
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Sigma54_activat	PF00158.28	2.4e-69	195..362
HTH_8	PF02954.21	1.9e-13	454..493

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
		-	-

Protein sequence: 504 a.a.

						>T6CP012537 NC_017548:c3766490-3764979 [Pseudomonas aeruginosa M18] [Sfa2]
MFSRVPQPLAFAEALLAQFGALSRETDITALLGGFARAVGELSGCELSQLYLLDQTHTALTLNAECRDGLLQALEPSSLP
ADYTGEQLLQFALCQNRVVSLVDLPSRLHETGFLPAGEQPWLSLLCVPLVSPEKTVEGMVVCATRRPLDLQGFAHPLGQL
GSFVLAQLHLLQRLSQPSGEPQGPSSAPVVSGYGLIGRSAAMRETCRLISKVLHNPYTVLLQGETGTGKEVVARAIHEYG
PRRAQAFVVQNCAAFPENLLESELFGYRKGAFTGAERDRPGLFDAADGGTLLLDEIGDMPLSLQAKLLRVLQEGEIRPLG
GNDTHKVDVRIIAATHRDLALLVRDGKFREDLYYRLAQFPIELPPLRQREGDALELARHFAERACVFLRRAPLGWSAEAL
ELLDGYGFPGNVRELKGLVECAVLLCEGEVLLAEHFSLRAPPEAEAPLKLREHLEQIERDLLLACLRRNAGNQTLAAREL
GLPRRTLIYRMGRLNIHFGDPRT*					

Nucleotide sequence: 1512 bp

						>T6CP012537 NC_017548:c3766490-3764979 [Pseudomonas aeruginosa M18] [Sfa2]
ATGTTCAGCCGCGTACCGCAACCCCTGGCCTTCGCCGAAGCCCTGCTCGCTCAGTTCGGCGCGCTGTCGCGGGAAACCGA
CATCACGGCCCTGCTGGGCGGTTTCGCACGCGCAGTCGGCGAACTCAGCGGCTGCGAGCTGAGCCAGTTGTATCTGCTCG
ACCAGACCCATACCGCGCTCACCCTCAACGCCGAATGCCGGGACGGCCTGTTGCAGGCGCTGGAGCCGTCGAGCCTGCCG
GCGGACTACACCGGCGAGCAGTTGCTGCAGTTCGCCCTGTGCCAGAACCGCGTGGTCAGTCTCGTCGACCTGCCGTCGCG
GCTGCACGAGACCGGCTTCCTGCCGGCAGGGGAGCAGCCCTGGCTGTCCCTGCTCTGCGTTCCGCTGGTGAGCCCGGAGA
AAACGGTCGAGGGCATGGTGGTCTGCGCTACCCGGCGCCCACTGGACCTGCAGGGATTCGCCCATCCACTGGGCCAGCTG
GGCTCGTTCGTGCTGGCCCAGCTGCATCTGCTGCAACGCCTCTCGCAACCCTCCGGCGAGCCGCAGGGACCGTCCAGCGC
ACCGGTGGTCAGCGGCTACGGGCTGATCGGCAGGAGCGCGGCGATGCGTGAGACCTGCCGCCTGATCAGCAAGGTCCTGC
ACAACCCCTATACCGTGTTGCTGCAGGGCGAGACCGGCACCGGCAAGGAGGTGGTCGCACGGGCGATCCACGAGTACGGC
CCGCGGCGTGCACAGGCATTCGTGGTGCAGAACTGCGCGGCGTTTCCGGAAAACCTGCTGGAAAGCGAGCTGTTCGGCTA
CCGCAAGGGCGCCTTTACCGGTGCCGAGCGCGATCGTCCCGGGTTGTTCGATGCCGCGGATGGCGGAACCCTGTTGCTCG
ACGAAATCGGCGACATGCCCCTGTCGCTCCAGGCCAAGCTGTTGCGCGTGTTGCAGGAAGGCGAGATTCGCCCGCTCGGC
GGCAACGACACGCACAAGGTCGATGTACGCATCATCGCCGCTACCCACCGTGACCTCGCCCTGCTGGTCCGCGATGGCAA
GTTCCGCGAAGACCTCTACTACCGGCTGGCGCAGTTTCCCATCGAGTTGCCGCCGTTGCGCCAGCGCGAGGGCGACGCCC
TCGAACTGGCCCGGCATTTCGCCGAGCGCGCCTGCGTGTTCCTACGCCGCGCGCCGCTGGGCTGGTCCGCGGAAGCGCTG
GAACTGCTCGACGGCTATGGCTTTCCCGGCAACGTTCGGGAGCTGAAGGGACTGGTGGAATGCGCGGTACTGCTCTGCGA
AGGCGAGGTGCTGCTGGCCGAACACTTTTCCCTGCGCGCGCCCCCGGAAGCGGAGGCGCCGCTCAAGCTGCGCGAGCATC
TGGAGCAGATCGAACGGGACCTGCTGCTCGCCTGCCTGCGCAGGAACGCTGGCAACCAGACCCTCGCCGCGCGCGAACTG
GGTCTGCCGCGACGAACCCTGATCTATCGCATGGGACGCCTGAACATTCATTTCGGCGATCCCCGGACCTGA					