Detailed information of component protein


Summary

Component ID T6CP012527
Component protein type Sfa2
T6SS ID (Type) T6SS00541 insolico (Type i1)
Strain Pseudomonas fluorescens F113
Replicon chromosome
Sequence Protein sequence (506 a.a.); Nucleotide sequence (1518 bp)
Reference
insolico Bioinformatics investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSF113_RS59950
Coordinate (Strand) 6716055..6717572 (-)
NCBI ID WP_014340905.1
RefSeq NC_016830
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Sigma54_activat PF00158.28 6.3e-69 196..362
HTH_8 PF02954.21 1.2e-11 456..495
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-505 MFKKLSQPLDYAEALLAQFSDLSRLADGAELVGKFIQGVARLSGCELVQLYLLDATRTRLEMNTECLEGQLQFRDPKSLPSDYHSEQLLQFSLCQNRVVCLDALSDSVHETGFLPPRARPWQSLLCVPLGDPQDGVAGLLLCASNQHLDLHGIAESLGHLGTFALGQMHLLQRLRGPVDESEPDTTSLPSAVSYGLIGKSMAMRKTCSLLSKVLHSPYTVLLRGETGTGKEVVARVLHDYGPRRSKAFIVQNCAAVPENLLESELFGYRKGAFTGADRDRAGLFDAANGGTLLLDEIGDMPLSLQAKLLRVLQEGEIRPLGSNVTHHVDVRIIAATHRDLKQLMEEGKFREDLYYRLAQFPIPLPALRQRDGDIIELARHFADKACTLLQRDPVRWSDAALDHLAAYDFPGNVRELKSIVERAVLLCEGGELLVEHFALHIQTPAERGRVSLRERMEQIERGLLIDGLRESSGNKARAARELGLPLRTLVYRLARLNVQRSDFKD


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9787 - -


  Protein sequence: 506 a.a.    

>T6CP012527 NC_016830:c6717572-6716055 [Pseudomonas fluorescens F113] [Sfa2]
MFKKLSQPLDYAEALLAQFSDLSRLADGAELVGKFIQGVARLSGCELVQLYLLDATRTRLEMNTECLEGQLQFRDPKSLP
SDYHSEQLLQFSLCQNRVVCLDALSDSVHETGFLPPRARPWQSLLCVPLGDPQDGVAGLLLCASNQHLDLHGIAESLGHL
GTFALGQMHLLQRLRGPVDESEPDTTSLPSAVSYGLIGKSMAMRKTCSLLSKVLHSPYTVLLRGETGTGKEVVARVLHDY
GPRRSKAFIVQNCAAVPENLLESELFGYRKGAFTGADRDRAGLFDAANGGTLLLDEIGDMPLSLQAKLLRVLQEGEIRPL
GSNVTHHVDVRIIAATHRDLKQLMEEGKFREDLYYRLAQFPIPLPALRQRDGDIIELARHFADKACTLLQRDPVRWSDAA
LDHLAAYDFPGNVRELKSIVERAVLLCEGGELLVEHFALHIQTPAERGRVSLRERMEQIERGLLIDGLRESSGNKARAAR
ELGLPLRTLVYRLARLNVQRSDFKD*

  Nucleotide sequence: 1518 bp    

>T6CP012527 NC_016830:c6717572-6716055 [Pseudomonas fluorescens F113] [Sfa2]
ATGTTCAAGAAACTCTCGCAACCGTTGGACTATGCCGAAGCGCTTCTGGCGCAGTTTTCCGACCTTTCACGTTTGGCGGA
CGGTGCTGAGCTGGTGGGGAAGTTCATCCAGGGCGTAGCGCGGCTCAGTGGCTGTGAACTGGTGCAGTTGTACCTGCTCG
ATGCCACTCGCACCCGCCTGGAAATGAACACCGAGTGCCTGGAAGGGCAGTTGCAATTTCGTGACCCGAAAAGCCTGCCC
TCCGACTACCACTCCGAGCAATTGTTGCAATTTTCCCTGTGCCAGAACCGTGTGGTGTGCCTCGACGCGTTAAGCGACAG
CGTGCACGAAACCGGTTTCCTGCCGCCGCGGGCCAGACCCTGGCAGTCGCTGCTTTGCGTTCCCCTGGGCGATCCGCAGG
ATGGGGTCGCAGGCTTGTTGCTATGCGCGAGCAATCAGCACCTCGACCTGCATGGTATCGCTGAGTCCCTGGGGCATCTT
GGGACCTTCGCGCTTGGTCAGATGCATCTGTTACAGCGCCTGCGCGGTCCTGTTGATGAGTCAGAGCCTGATACCACAAG
CCTGCCGAGCGCCGTCAGTTACGGCCTGATTGGCAAGAGCATGGCCATGCGCAAGACCTGCTCATTGCTCAGTAAAGTAC
TGCACAGCCCCTATACCGTCCTGCTGCGGGGTGAGACCGGTACCGGCAAGGAGGTGGTGGCCCGGGTCCTCCATGATTAC
GGCCCGCGTCGATCCAAGGCGTTTATCGTGCAGAACTGTGCGGCGGTTCCCGAGAACTTGTTGGAGAGTGAGCTTTTCGG
CTATCGCAAGGGCGCGTTCACCGGTGCCGACCGGGACCGGGCCGGGCTGTTCGACGCGGCCAACGGCGGCACGCTGTTGC
TCGATGAAATCGGCGATATGCCGTTATCCCTGCAGGCCAAGTTGTTGCGAGTGTTGCAGGAAGGCGAAATCCGGCCGTTG
GGGTCCAACGTCACTCACCATGTCGACGTGCGCATCATAGCCGCCACCCACCGCGACCTGAAGCAACTGATGGAGGAGGG
CAAGTTCCGCGAAGACCTGTATTACCGCCTTGCGCAGTTTCCAATTCCGCTACCGGCGTTGCGCCAGCGCGACGGCGACA
TCATTGAGCTGGCGCGGCATTTTGCCGACAAGGCTTGCACACTCCTGCAGCGTGATCCGGTGCGCTGGTCAGACGCAGCG
CTCGATCACCTGGCTGCCTACGACTTCCCGGGAAACGTCCGTGAACTCAAGAGCATCGTAGAAAGGGCCGTGCTCTTGTG
CGAGGGCGGCGAACTGCTTGTCGAGCATTTTGCACTACACATCCAGACCCCAGCCGAGCGGGGCCGTGTGAGCTTGCGTG
AACGGATGGAGCAGATCGAACGTGGCCTGCTGATCGATGGCCTGCGCGAGAGTTCGGGCAACAAGGCGCGTGCCGCCCGT
GAACTTGGCTTGCCGCTACGCACGCTGGTCTATCGCCTTGCGCGCCTGAACGTTCAGCGTAGTGATTTCAAAGATTGA