SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP012483
Component protein type	VasH
T6SS ID (Type)	T6SS00216 (Type i1)
Strain	Marinomonas sp. MWYL1
Replicon	chromosome
Sequence	Protein sequence (517 a.a.); Nucleotide sequence (1551 bp)
Reference	[1] Bernard CS, Brunet YR, Gavioli M, et al. Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins. J Bacteriol. 2011 May;193(9):2158-67. doi: 10.1128/JB.00029-11. Epub 2011 Mar 4. PMID: 21378190

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	MMWYL1_RS06115
Coordinate (Strand)	1348733..1350283 (+)
NCBI ID	WP_012068921.1
RefSeq	NC_009654
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Sigma54_activat	PF00158.28	1e-66	194..360

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
		-	-

Protein sequence: 517 a.a.

						>T6CP012483 NC_009654:1348733-1350283 [Marinomonas sp. MWYL1] [VasH]
MNNWLKSAAELVVIRSTAQLLSTFTQVLDSELGCRGVYVLTPSADGRFVESSMNDSRQLSWSVSDFDNPFAHVIHSGKVM
ILRNSELLYWLTNDSFADLIGCTDETVNVIILPMLSKDGQLQAILVLKTDSYSNYDVDSDENFLGLLSVCANHLQLLNDM
EAKKRKSQLLSESLIEVEQGKKKNHLLDGLANVLIGQSDVMKSLREQVTIAASSRLSVMIQGETGTGKELVSRAVHDLSS
RSKANFVAINCAAIPEHLLESELFGYVKGAFSGADKSKKGLLADADGGTLFLDEIGDMPLALQAKLLRVLESHKYRPVGS
EKELDSNFRLVVATHLNLKQQVLDNKFRKDLYYRLYQYPITLPRLKDRKSDIEKLSAHFVEQYNAKHKSSVRGLHYKAMD
CLLDYDFPGNVRELKHLIEYGAAQTQNGEQIELAYIQLKLDSEMASSSQPEPKNRALVELSTIRDLKVAVREYESNIIAA
RLREFDGDRGRTAESLGIPKRTLADKCLKLEISIND*					

Nucleotide sequence: 1551 bp

						>T6CP012483 NC_009654:1348733-1350283 [Marinomonas sp. MWYL1] [VasH]
ATGAATAACTGGCTTAAGTCTGCAGCTGAGTTGGTGGTGATTCGTAGCACCGCTCAGTTGTTGTCAACATTCACGCAAGT
ACTTGATAGCGAGCTAGGGTGCCGAGGGGTTTATGTCCTAACCCCTTCTGCTGATGGGCGCTTTGTAGAATCGAGCATGA
ATGATAGTCGTCAATTATCTTGGTCGGTTTCTGACTTTGATAATCCATTTGCCCACGTCATTCATTCAGGCAAAGTCATG
ATACTGCGTAACAGCGAATTGCTTTACTGGTTAACGAATGACAGTTTTGCTGATCTGATTGGTTGTACTGATGAAACCGT
AAACGTCATCATTTTGCCCATGTTGTCAAAAGATGGGCAGCTTCAGGCGATCTTGGTTTTAAAAACGGATAGTTACTCGA
ATTATGATGTGGACAGCGATGAAAATTTTTTAGGTCTGCTGTCTGTTTGTGCGAATCATTTACAGTTACTTAATGATATG
GAAGCAAAGAAAAGGAAGTCACAACTGCTGTCAGAGTCATTGATAGAAGTGGAACAAGGTAAGAAAAAGAACCATCTCTT
GGATGGACTTGCCAACGTATTAATTGGTCAAAGTGACGTAATGAAATCGCTGCGTGAACAGGTGACCATTGCTGCATCAT
CCCGTCTTTCAGTGATGATTCAAGGAGAGACAGGTACGGGGAAGGAGTTAGTGTCGCGTGCTGTCCATGATTTGTCCTCA
CGTAGTAAAGCAAATTTTGTCGCTATAAATTGTGCTGCTATCCCTGAGCATTTGCTGGAAAGCGAGTTGTTTGGCTACGT
CAAAGGCGCATTTTCCGGTGCAGATAAAAGTAAAAAAGGGCTGTTAGCCGATGCGGACGGGGGCACTCTATTCCTGGATG
AAATCGGAGATATGCCGTTAGCTTTGCAAGCGAAATTGCTTAGAGTATTGGAATCTCATAAATATCGCCCAGTAGGATCT
GAAAAAGAATTGGATTCTAACTTTAGATTAGTGGTGGCTACTCATTTAAATCTAAAACAACAGGTATTGGATAATAAGTT
CCGCAAGGATTTGTATTATCGCTTATATCAATACCCCATCACTTTGCCGCGTTTAAAAGATCGCAAGTCAGATATTGAAA
AACTCAGTGCGCATTTTGTTGAACAATATAATGCCAAGCACAAATCCAGCGTGCGTGGTCTGCATTATAAAGCGATGGAT
TGCTTACTCGATTATGACTTCCCTGGAAATGTTCGAGAATTAAAACATCTTATTGAATATGGTGCTGCTCAGACGCAAAA
CGGTGAGCAAATTGAACTGGCCTACATTCAATTGAAACTTGACAGTGAAATGGCTAGCTCAAGTCAACCTGAGCCAAAAA
ATAGAGCTTTGGTTGAGCTTTCCACCATTCGAGATCTTAAAGTGGCGGTAAGGGAATATGAGTCCAATATCATTGCTGCT
CGATTGCGTGAATTTGATGGAGATAGAGGACGAACTGCTGAGAGTTTGGGCATACCCAAACGAACCTTGGCAGATAAGTG
TTTGAAATTGGAGATTTCCATAAATGATTAG					