SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP012425
Component protein type	TagT
T6SS ID (Type)	T6SS00013 (Type i1)
Strain	Pseudomonas putida KT2440
Replicon	chromosome
Sequence	Protein sequence (618 a.a.); Nucleotide sequence (1854 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537 [3] Bernal P, Allsopp LP, Filloux A, et al. The Pseudomonas putida T6SS is a plant warden against phytopathogens. ISME J. 2017 Apr;11(4):972-987. doi: 10.1038/ismej.2016.169. Epub 2017 Jan 3. PMID: 28045455

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PP_RS13700
Coordinate (Strand)	3007118..3008971 (-)
NCBI ID	WP_028697022.1
RefSeq	NC_002947
Uniprot ID	A0A2S3WMD4_PSEPU, A0A2V4FME8_9PSED
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ABC_tran	PF00005.29	1.2e-35	377..531
ABC_membrane	PF00664.25	3.6e-19	39..307

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-36	MLRIFERRLDPFPPDEVPPPPVGLLRFMWACTRGAR
Transmembrane helix	37-59	GYILALALLSAGVSIYEAWLFAF
Outside	60-82	LGQVVDLLASWQAGGTVGPEESR
Transmembrane helix	83-100	VLWGIGIVLVVSIGLVAL
Inside	101-154	RTMVQHQVLAINLPLRLRWDFHRLMLRQSLSFFSDEFSGRVTTKVMQTALAVRD
Transmembrane helix	155-177	VLFTLIEILPGIGVYFIAIIALA
Outside	178-186	GGFALKLML
Transmembrane helix	187-209	PFIAWVVLFGLAMLYFVPRLGKV
Inside	210-269	GQEQANARSSMTGRVSDAYTNITTVKLFSHSNREAHFARAAMEDFKQTGFRQMRLVSQFE
Transmembrane helix	270-292	IVNQALVVGLIFGAGGYALWLWH
Outside	293-617	QGQVGTGAVAAITAMALRVNGMSHWIMWEITSLFENIGTVQDGMATLTRGPKVQDVPGAAELVTTGGAVSFDKVSFNYNGERQVLNELTLHIRPGEKVGLVGRSGAGKSTLINLLLRFYDVDKGEIRIDGQNVAKVTQDSLRSAIGMVTQDTSLLHRSIRDNIAYGRPDATEAQIRAAAASAQADGFISQLSDRQGNSGYDTLVGERGIKLSGGQRQRIAIARVMLKNAPILLLDEATSALDSEVEVAIQESLDEMMQGKTVIAIAHRLSTIAAMDRLIVMDEGRIIEQGTHAELLAKKGTYARLWQHQSGGFLGEDQGVAEAVE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9905	-	-

Protein sequence: 618 a.a.

						>T6CP012425 NC_002947:c3008971-3007118 [Pseudomonas putida KT2440] [TagT]
MLRIFERRLDPFPPDEVPPPPVGLLRFMWACTRGARGYILALALLSAGVSIYEAWLFAFLGQVVDLLASWQAGGTVGPEE
SRVLWGIGIVLVVSIGLVALRTMVQHQVLAINLPLRLRWDFHRLMLRQSLSFFSDEFSGRVTTKVMQTALAVRDVLFTLI
EILPGIGVYFIAIIALAGGFALKLMLPFIAWVVLFGLAMLYFVPRLGKVGQEQANARSSMTGRVSDAYTNITTVKLFSHS
NREAHFARAAMEDFKQTGFRQMRLVSQFEIVNQALVVGLIFGAGGYALWLWHQGQVGTGAVAAITAMALRVNGMSHWIMW
EITSLFENIGTVQDGMATLTRGPKVQDVPGAAELVTTGGAVSFDKVSFNYNGERQVLNELTLHIRPGEKVGLVGRSGAGK
STLINLLLRFYDVDKGEIRIDGQNVAKVTQDSLRSAIGMVTQDTSLLHRSIRDNIAYGRPDATEAQIRAAAASAQADGFI
SQLSDRQGNSGYDTLVGERGIKLSGGQRQRIAIARVMLKNAPILLLDEATSALDSEVEVAIQESLDEMMQGKTVIAIAHR
LSTIAAMDRLIVMDEGRIIEQGTHAELLAKKGTYARLWQHQSGGFLGEDQGVAEAVE*					

Nucleotide sequence: 1854 bp

						>T6CP012425 NC_002947:c3008971-3007118 [Pseudomonas putida KT2440] [TagT]
ATGCTGCGTATCTTTGAACGTCGGCTCGACCCCTTCCCACCCGACGAGGTACCACCGCCGCCCGTTGGCCTGTTGCGCTT
CATGTGGGCTTGTACCCGCGGCGCCCGTGGCTACATCCTGGCGCTGGCGCTGCTCAGTGCCGGGGTGTCGATATACGAAG
CCTGGCTATTCGCGTTCCTGGGCCAGGTGGTCGACCTGCTCGCGTCCTGGCAGGCAGGCGGTACTGTTGGCCCCGAGGAA
AGCCGTGTGCTGTGGGGGATCGGCATCGTCCTGGTGGTCAGCATCGGGCTGGTGGCGCTGCGCACCATGGTTCAGCACCA
GGTGCTGGCGATCAACCTGCCGCTGCGGCTGCGTTGGGATTTTCACCGCCTGATGTTGCGCCAGAGCTTATCGTTCTTTT
CTGACGAGTTTTCTGGCCGGGTCACTACCAAGGTGATGCAGACGGCGCTGGCAGTACGAGATGTGCTGTTTACACTCATT
GAAATCCTGCCTGGGATTGGCGTGTATTTCATCGCGATCATTGCCCTGGCCGGCGGCTTTGCCCTGAAACTGATGCTGCC
CTTCATTGCCTGGGTCGTGCTGTTCGGCTTGGCCATGCTGTATTTCGTCCCCCGGCTGGGCAAGGTCGGGCAGGAGCAGG
CCAATGCCCGGTCGTCGATGACCGGGCGGGTTTCTGACGCTTACACCAACATCACCACGGTAAAACTGTTTTCGCACTCC
AACCGCGAAGCGCACTTTGCGCGCGCAGCCATGGAAGATTTCAAGCAGACCGGTTTTCGCCAGATGCGCCTGGTGAGCCA
GTTCGAAATCGTCAACCAGGCGTTGGTGGTGGGCTTGATCTTTGGTGCCGGTGGCTACGCACTGTGGCTGTGGCACCAGG
GCCAGGTTGGCACCGGGGCCGTGGCGGCAATCACCGCCATGGCCTTGCGCGTCAACGGTATGTCGCACTGGATCATGTGG
GAAATTACCTCGCTGTTCGAAAACATCGGCACCGTGCAGGACGGTATGGCCACCCTCACCCGCGGCCCCAAGGTGCAGGA
TGTGCCGGGTGCTGCAGAGCTGGTAACCACGGGTGGCGCGGTGAGTTTCGACAAGGTCAGCTTCAACTACAACGGCGAGC
GCCAGGTGCTCAATGAGCTGACGCTGCACATTCGTCCGGGCGAAAAAGTCGGCCTGGTCGGCCGCTCCGGCGCCGGCAAG
TCCACCTTGATCAACCTGCTGCTGCGCTTCTACGACGTGGACAAGGGGGAAATACGCATCGACGGGCAGAACGTCGCCAA
GGTCACGCAGGATAGCCTGCGCAGCGCCATCGGCATGGTCACTCAGGACACCTCGCTGTTGCACCGTTCGATTCGCGACA
ATATTGCCTATGGCCGCCCCGATGCTACCGAAGCACAGATCCGTGCCGCTGCGGCCAGTGCCCAGGCCGATGGTTTCATC
AGCCAGCTCAGCGACCGCCAGGGCAACAGCGGTTACGACACCCTGGTGGGTGAACGCGGCATCAAGCTGTCTGGCGGCCA
ACGCCAGCGCATTGCAATTGCCCGGGTGATGCTGAAGAACGCACCGATCCTGCTGCTTGACGAAGCCACCAGCGCGCTGG
ACTCGGAGGTGGAGGTGGCCATTCAGGAGAGCCTGGATGAAATGATGCAAGGCAAGACCGTTATTGCCATCGCCCACCGG
TTGTCGACGATCGCCGCCATGGACCGGCTGATCGTGATGGATGAAGGGCGCATCATCGAGCAAGGCACCCATGCCGAACT
GTTGGCGAAAAAGGGTACCTATGCACGGTTGTGGCAGCATCAGAGTGGCGGGTTCCTGGGTGAGGATCAGGGCGTGGCCG
AGGCTGTGGAGTAA					