Detailed information of component protein


Summary

Component ID T6CP012421
Component protein type Sfa2
T6SS ID (Type) T6SS00004 experimental (Type i1)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome (H2-T6SS)
Sequence Protein sequence (504 a.a.); Nucleotide sequence (1512 bp)
Reference
[1] Sana TG, Hachani A, Bucior I, et al. The second type VI secretion system of Pseudomonas aeruginosa strain PAO1 is regulated by quorum sensing and Fur and modulates internalization in epithelial cells. J Biol Chem. 2012 Aug 3;287(32):27095-105. doi: 10.1074/jbc.M112.376368. Epub 2012 Jun 4. PMID: 22665491
[2] Osipiuk J, Xu X, Cui H, et al. Crystal structure of secretory protein Hcp3 from Pseudomonas aeruginosa. J Struct Funct Genomics. 2011 Mar;12(1):21-6. doi: 10.1007/s10969-011-9107-1. Epub 2011 Apr 8. PMID: 21476004
[3] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
[4] Hachani A, Lossi NS, Hamilton A, et al. Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins. J Biol Chem. 2011 Apr 8;286(14):12317-27. doi: 10.1074/jbc.M110.193045. Epub 2011 Feb 16. PMID: 21325275
[5] Bleves S, Viarre V, Salacha R, et al. Protein secretion systems in Pseudomonas aeruginosa: A wealth of pathogenic weapons. Int J Med Microbiol. 2010 Dec;300(8):534-43. doi: 10.1016/j.ijmm.2010.08.005. Epub 2010 Oct 13. PMID: 20947426
[6] Russell AB, LeRoux M, Hathazi K, et al. Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 2013 Apr 25;496(7446):508-12. doi: 10.1038/nature12074. Epub 2013 Apr 3. PMID: 23552891
[7] Sana TG, Soscia C, Tonglet CM, et al. Divergent control of two type VI secretion systems by RpoN in Pseudomonas aeruginosa. PLoS One. 2013 Oct 21;8(10):e76030. doi: 10.1371/journal.pone.0076030. eCollection 2013. PMID: 24204589
[8] Jiang F, Waterfield NR, Yang J, et al. A Pseudomonas aeruginosa type VI secretion phospholipase D effector targets both prokaryotic and eukaryotic cells. Cell Host Microbe. 2014 May 14;15(5):600-10. doi: 10.1016/j.chom.2014.04.010. PMID: 24832454
[9] Sana TG, Baumann C, Merdes A, et al. Internalization of Pseudomonas aeruginosa Strain PAO1 into Epithelial Cells Is Promoted by Interaction of a T6SS Effector with the Microtubule Network. mBio. 2015 Jun 2;6(3):e00712. doi: 10.1128/mBio.00712-15. PMID: 26037124
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PA1663
Coordinate (Strand) 1813395..1814906 (+)
NCBI ID NP_250354.1
RefSeq NC_002516
Uniprot ID Q9I362_PSEAE
KEGG ID pae:PA1663
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Sigma54_activat PF00158.28 2.4e-69 195..362
HTH_8 PF02954.21 1.9e-13 454..493
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-503 MFSRVPQPLAFAEALLAQFGALSRETDITALLGGFARAVGELSGCELSQLYLLDQTHTALTLNAECRDGLLQALEPSSLPADYTGEQLLQFALCQNRVVSLVDLPSRLHETGFLPAGEQPWLSLLCVPLVSPEKTVEGMVVCATRRPLDLQGFAHPLGQLGSFVLAQLHLLQRLSQPSGEPQGPSSAPVVSGYGLIGRSAAMRETCRLISKVLHNPYTVLLQGETGTGKEVVARAIHEYGPRRAQAFVVQNCAAFPENLLESELFGYRKGAFTGAERDRPGLFDAADGGTLLLDEIGDMPLSLQAKLLRVLQEGEIRPLGGNDTHKVDVRIIAATHRDLALLVRDGKFREDLYYRLAQFPIELPPLRQREGDALELARHFAERACVFLRRAPLGWSAEALELLDGYGFPGNVRELKGLVECAVLLCEGEMLLAEHFSLRAPPEAEAPLKLREHLEQIERDLLLACLRRNAGNQTLAARELGLPRRTLIYRMGRLNIHFGDPRT


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.7749 - -


  Protein sequence: 504 a.a.    

>T6CP012421 NC_002516:1813395-1814906 [Pseudomonas aeruginosa PAO1] [Sfa2]
MFSRVPQPLAFAEALLAQFGALSRETDITALLGGFARAVGELSGCELSQLYLLDQTHTALTLNAECRDGLLQALEPSSLP
ADYTGEQLLQFALCQNRVVSLVDLPSRLHETGFLPAGEQPWLSLLCVPLVSPEKTVEGMVVCATRRPLDLQGFAHPLGQL
GSFVLAQLHLLQRLSQPSGEPQGPSSAPVVSGYGLIGRSAAMRETCRLISKVLHNPYTVLLQGETGTGKEVVARAIHEYG
PRRAQAFVVQNCAAFPENLLESELFGYRKGAFTGAERDRPGLFDAADGGTLLLDEIGDMPLSLQAKLLRVLQEGEIRPLG
GNDTHKVDVRIIAATHRDLALLVRDGKFREDLYYRLAQFPIELPPLRQREGDALELARHFAERACVFLRRAPLGWSAEAL
ELLDGYGFPGNVRELKGLVECAVLLCEGEMLLAEHFSLRAPPEAEAPLKLREHLEQIERDLLLACLRRNAGNQTLAAREL
GLPRRTLIYRMGRLNIHFGDPRT*

  Nucleotide sequence: 1512 bp    

>T6CP012421 NC_002516:1813395-1814906 [Pseudomonas aeruginosa PAO1] [Sfa2]
ATGTTCAGCCGCGTACCGCAACCCCTGGCCTTCGCCGAAGCCCTGCTCGCTCAGTTCGGCGCGCTGTCGCGGGAAACCGA
CATCACGGCCCTGCTGGGCGGTTTCGCACGCGCAGTCGGCGAACTCAGCGGCTGCGAGCTGAGCCAGTTGTATCTGCTCG
ACCAGACCCATACCGCGCTCACCCTCAACGCCGAATGCCGGGACGGCCTGTTGCAGGCGCTGGAGCCGTCGAGCCTGCCG
GCGGACTACACCGGCGAGCAGTTGCTGCAGTTCGCCCTGTGCCAGAACCGCGTGGTCAGTCTCGTCGACCTGCCGTCGCG
GCTGCACGAGACCGGCTTCCTGCCGGCAGGGGAGCAGCCCTGGCTGTCCCTGCTCTGCGTTCCGCTGGTGAGCCCGGAGA
AAACGGTCGAGGGCATGGTGGTCTGCGCTACCCGGCGGCCATTGGACCTGCAGGGATTCGCCCATCCACTGGGCCAGCTG
GGCTCGTTCGTGCTGGCCCAGCTGCATCTGCTGCAACGCCTCTCGCAACCCTCCGGCGAGCCGCAGGGACCGTCCAGCGC
ACCGGTGGTCAGCGGCTACGGGCTGATCGGCAGGAGCGCGGCGATGCGTGAGACCTGCCGCCTGATCAGCAAGGTCCTGC
ACAACCCCTATACCGTGTTGCTGCAGGGCGAGACCGGCACCGGCAAGGAGGTGGTCGCACGGGCGATCCACGAGTACGGC
CCGCGGCGTGCACAGGCGTTCGTGGTGCAGAACTGCGCGGCGTTTCCGGAAAACCTGCTGGAAAGCGAGCTGTTCGGCTA
CCGCAAGGGCGCCTTTACCGGTGCCGAGCGCGATCGTCCCGGGTTGTTCGATGCCGCGGATGGCGGAACCCTGCTGCTCG
ACGAAATCGGCGACATGCCCCTCTCGCTCCAGGCCAAGCTGTTGCGCGTGTTGCAGGAAGGCGAGATTCGCCCGCTCGGC
GGCAACGATACGCACAAGGTCGATGTACGCATCATCGCCGCTACCCACCGTGACCTCGCCCTGCTGGTACGCGATGGCAA
GTTCCGCGAAGACCTCTACTACCGGCTGGCGCAGTTTCCCATCGAGTTGCCGCCGTTGCGCCAGCGCGAGGGCGACGCCC
TCGAACTGGCCCGGCATTTCGCCGAGCGCGCCTGCGTGTTCCTGCGCCGCGCGCCGCTGGGCTGGTCCGCGGAAGCGCTG
GAACTGCTCGACGGCTATGGCTTTCCCGGCAACGTTCGGGAGCTGAAGGGATTGGTGGAATGCGCGGTACTGCTCTGCGA
AGGCGAGATGCTGCTGGCCGAACACTTTTCCCTGCGCGCGCCCCCGGAAGCGGAGGCGCCGCTCAAGCTGCGCGAGCATC
TGGAGCAGATCGAACGGGACCTGCTGCTCGCCTGCCTGCGCAGGAACGCTGGCAACCAGACCCTCGCCGCGCGCGAACTG
GGTCTGCCGCGACGAACCCTGATCTATCGCATGGGACGCCTGAACATTCATTTCGGCGATCCCCGGACCTGA