Detailed information of component protein


Summary

Component ID T6CP012399
Component protein type TagL
T6SS ID (Type) T6SS00904 experimental (Type i2)
Strain Escherichia coli strain TW-XM
Replicon chromosome
Sequence Protein sequence (538 a.a.); Nucleotide sequence (1614 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) -
Coordinate (Strand) 4167..5780 (+)
NCBI ID 611963683
GenBank KF678349
Uniprot ID X5FV56_ECOLX
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
OmpA PF00691.22 7.2e-17 424..520
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-14 MVLLLVLWSVFISP
Transmembrane helix 15-37 SGVLRWAGAAAIVLAVAALLIYR
Inside 38-303 RRQAWTEMTGDAGLSSLPPETYRQPVVLVCGGLSAHLFTDSPVRQVSEGLYLHVPDEEQLVAQVERLLTLRPAWASQLAVAYTIMPGIHRDVAVLAGRLRRFAHSMATVRRRAGVNVPWLLWSGLSGSPLPERASSPWFICTGGEVQVATSTENAMPAQWIAQSGVQERSQRLSYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMAVGLVPSLPAVDNNLWQLWITARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRR
Transmembrane helix 304-326 ACVTMLGVTTVAGIAALCLSATA
Outside 327-537 NRQLLRQVGDDLHRFYAVPVEEFITKARHLSVLKDDATMLDGYYREGEPLRLGLGLYPGERIRQPVLRAIRDWRPPEQKMEVTASLQVQTVRLDSMSLFDVGQARLKDGSTKVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATCFAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9730 - -


  Protein sequence: 538 a.a.    

>T6CP012399 KF678349:4167-5780 [Escherichia coli] [TagL]
MVLLLVLWSVFISPSGVLRWAGAAAIVLAVAALLIYRRRQAWTEMTGDAGLSSLPPETYRQPVVLVCGGLSAHLFTDSPV
RQVSEGLYLHVPDEEQLVAQVERLLTLRPAWASQLAVAYTIMPGIHRDVAVLAGRLRRFAHSMATVRRRAGVNVPWLLWS
GLSGSPLPERASSPWFICTGGEVQVATSTENAMPAQWIAQSGVQERSQRLSYLLKAESLMQWLDLNVLAELNGPEAKCPP
LAMAVGLVPSLPAVDNNLWQLWITARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRRACVTMLGVTTVAGIAAL
CLSATANRQLLRQVGDDLHRFYAVPVEEFITKARHLSVLKDDATMLDGYYREGEPLRLGLGLYPGERIRQPVLRAIRDWR
PPEQKMEVTASLQVQTVRLDSMSLFDVGQARLKDGSTKVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEA
VRNWMLQTSDIPATCFAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK*

  Nucleotide sequence: 1614 bp    

>T6CP012399 KF678349:4167-5780 [Escherichia coli] [TagL]
ATGGTGTTACTGCTGGTGCTGTGGTCAGTGTTTATCAGTCCGTCTGGCGTGCTGAGATGGGCCGGTGCGGCGGCTATCGT
TCTGGCGGTTGCTGCGTTGTTGATTTATCGGCGCAGGCAGGCGTGGACGGAGATGACCGGCGATGCCGGGTTGTCATCGC
TGCCGCCGGAAACCTACCGACAGCCGGTAGTGCTGGTCTGTGGCGGTCTGTCGGCGCACCTGTTCACTGACAGCCCGGTC
CGCCAGGTTTCAGAAGGGCTGTATCTGCATGTTCCTGATGAAGAACAGCTTGTGGCGCAGGTGGAGCGATTGCTGACCCT
TCGCCCGGCGTGGGCATCGCAGCTTGCCGTGGCGTATACCATCATGCCCGGCATACACCGGGATGTGGCGGTTCTGGCCG
GACGGCTGCGACGGTTCGCCCACAGTATGGCGACGGTGCGTCGTCGGGCAGGCGTAAACGTCCCCTGGCTTCTCTGGAGC
GGGCTGTCCGGCTCGCCGTTGCCGGAAAGAGCGAGTTCACCGTGGTTTATCTGTACCGGCGGCGAAGTTCAGGTAGCAAC
ATCCACAGAGAACGCGATGCCCGCGCAGTGGATTGCACAATCCGGCGTACAGGAACGCAGTCAGCGACTCTCTTATCTGC
TGAAAGCCGAAAGCCTGATGCAGTGGCTGGATCTTAATGTGCTGGCGGAACTGAACGGCCCGGAGGCGAAATGTCCACCA
CTGGCGATGGCCGTGGGGCTGGTCCCCTCGTTGCCTGCGGTGGATAACAACCTGTGGCAGTTGTGGATCACCGCCAGAAC
CGGCCTGACGCCGGATATCGCGGACACCGGCACAGACGATGCGCTGCCATTCCCGGATGCCCTGTTACGGCGGTTGCCGC
GTCAGTCGGGCTTTACCCCGCTGCGACGGGCCTGCGTGACCATGCTGGGCGTCACCACCGTGGCGGGTATCGCCGCGCTG
TGCCTGTCAGCCACGGCAAATCGCCAGTTATTACGGCAGGTCGGTGACGATCTGCACCGGTTTTATGCCGTCCCGGTGGA
GGAATTTATCACCAAAGCCCGTCACCTGTCGGTGCTGAAAGACGATGCGACCATGCTCGATGGGTATTACCGGGAAGGAG
AACCACTGCGCCTCGGTCTGGGGTTATACCCCGGCGAACGCATCCGCCAGCCGGTATTACGCGCCATTCGCGACTGGCGT
CCGCCTGAACAAAAAATGGAGGTGACGGCTTCGCTTCAGGTTCAGACCGTGCGTCTTGACAGTATGTCGCTGTTTGACGT
CGGACAGGCCCGCCTGAAAGACGGCTCGACAAAAGTGCTGGTGGACGCACTGGTGAACATCCGGGCAAAACCGGGCTGGC
TGATCCTCGTGGCCGGATATACCGATGCCACCGGCGATGAAAAAAGCAATCAGCAGTTATCGCTGCGGCGTGCCGAAGCG
GTGCGCAACTGGATGCTGCAGACCAGCGACATCCCGGCCACCTGTTTTGCCGTACAGGGACTGGGCGAGAGCCAGCCTGC
GGCGACCAACGACACGCCACAGGGCCGGGCAGTCAACCGGCGTGTCGAAATCAGTCTTGTTCCGCGTTCTGACGCCTGTC
AGGACGTGAAATAA