SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP011991
Component protein type	PAAR
T6SS ID (Type)	T6SS00551 (Type i3)
Strain	Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
Replicon	chromosome
Sequence	Protein sequence (1365 a.a.); Nucleotide sequence (4095 bp)
Reference	[1] Liu J, Guo JT, Li YG, et al. The type VI secretion system gene cluster of Salmonella typhimurium: required for full virulence in mice. J Basic Microbiol. 2013 Jul;53(7):600-7. doi: 10.1002/jobm.201200047. Epub 2012 Sep 7. PMID: 22961625 [2] Pezoa D, Yang HJ, Blondel CJ, et al. The type VI secretion system encoded in SPI-6 plays a role in gastrointestinal colonization and systemic spread of Salmonella enterica serovar Typhimurium in the chicken. PLoS One. 2013 May 14;8(5):e63917. doi: 10.1371/journal.pone.0063917. Print 2013. PMID: 23691117

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	STM14_RS02045
Coordinate (Strand)	333236..337330 (+)
NCBI ID	WP_000272656.1
RefSeq	NC_016856
Uniprot ID	A0A5W4KAY6_SALTM, A0A4V1IDA0_SALET, A0A0W3N7H1_SALER, A0A2T8QLH5_SALET, A0A315GME1_SALET, A0A0F6AX94_SALT1, A0A3T6X4W2_SALET, A0A3V2ZGU8_SALET, A0A0J5DMN0_SALTM, A0A6C7APG4_SALTU, Q8ZRL0_SALTY
KEGG ID	seo:STM14_0340, sej:STMUK_0294, stm:STM0291
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DUF6531	PF20148.1	1.7e-21	224..296
PAAR_motif	PF05488.15	3.9e-14	72..138
RHS_repeat	PF05593.16	1.4e-10	582..619
RHS	PF03527.16	2.1e-09	1146..1177
RHS_repeat	PF05593.16	3.1e-07	624..660
RHS_repeat	PF05593.16	5.2e-07	518..555

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-16	MYEAARVDDPIYHTSA
Transmembrane helix	17-39	LAGFLIGAIIGIAIIALAAFAFF
Outside	40-1364	SCGFLAGLILGFMADQIASGVLQLGEAIGRSIHHTAGKILTGSENVSTNSRPAARAVLSTVKCDNHIAEKRIAQGSENIYINSQPAARKDDHTECDAVIEDGSPNVFLGGGTQTVLEISSEIPDWLRKVVDVLFVVASLLGGLAGAWRQAAKLGTKFGTKCAAKFIGGELVGMAVGEAISGLFSNPVDVTTGQKILLPETDFTLPGRLPVTCSRFYASHLETVGLLGRGWRLNWETSLRDDDEHITLTGVQGRELRYPKTMLTPGHQIFDPEEQLYLSRLHDGRYVLHYTDRSYYVFGDFDSDGMAYLLFMETPHRQRIVFGHEGGRLVRIASSSGHHLLLHRTQTPAGERLSRIELVQGGTRGNLVEYRYDDNGQLTGVVNRAGTQVRQFAYENGLMTAHSNATGFTCRYRWQELDGAPRVTEHDTSDGEHYRFDYDFAAGTTTVTGRQGETWQWWYDRETYITAHRTPGGGMYRFTYNEDHFPVNIELPGGRTVAYEYDIQNRVVKTTDPEGRVTQTQWNGEFDEITRTALDDDAVWKTQYNAHGQPVQETDPEGRVTQYAYDEQGQMCSRTDAAGGTVVTAFDSRGQMTRYTDCSGRSTGYDHDEDGNLTRVTDAEGKVVRISYNRLGLPETVNSPGKQQDRYTWNALGLMSSHRRITGSVESWRYTPRGLLAAHTDEEKRETRWQYTPEGRVAALTNGNGAQYRFSHDADGRLVREVRPDGLSRTFILDDSGYLTAIQTTGTQGGVRRETQQRDALGRLLRTENEHGQRTFSYNRLDQITAVTLTPTEAGQQQHRMQADTVRFEYDRSGWLTAEHAGNGSICYQRDALGNPTDITLPDGQHLTHLYYGSGHLLQTALDGLTVSEYERDSLHRQIMRTQGQLATYSGYDDDGLLSWQRSLASGSAPVLPGQRPARQGCVTSRDYYWNNHGEVGTIDDGLRGSVVYSYDRSGYLTGRSGQMYDHDRYYYDKAGNLLDNEGQGAVMSNRLPGCGRDRYGYNEWGELTTRRDQQLEWNAQGQLTRVISGNTETHYGYDALGRRTRKATYGRHTGHTARSRTDFVWEGFRLLQENVQQQGWRTYLYDAEQPYTPVASVTGRGESRQVWYYHTDVTGTPQEVTAADGTLVWAGYIRGFGENAADISNSGAYFHQPLRLPGQYFDDETGLHYNLFRYYAPECGRFVSQDPIGLRGGLNLYQYAPNPLKYIDPLGLTATVGRWMGPAEYQQMLDTGTVVQSSTGTTHVAYPADIDAFGKQAKNGAMYVEFDVPEKSLVPTNEGWAKIVGPDSIEGRLAKRKGLPVPEMPTAENITVRGEKINGEVEAKC

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9955	-	-

Protein sequence: 1365 a.a.

						>T6CP011991 NC_016856:333236-337330 [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S] [PAAR]
MYEAARVDDPIYHTSALAGFLIGAIIGIAIIALAAFAFFSCGFLAGLILGFMADQIASGVLQLGEAIGRSIHHTAGKILT
GSENVSTNSRPAARAVLSTVKCDNHIAEKRIAQGSENIYINSQPAARKDDHTECDAVIEDGSPNVFLGGGTQTVLEISSE
IPDWLRKVVDVLFVVASLLGGLAGAWRQAAKLGTKFGTKCAAKFIGGELVGMAVGEAISGLFSNPVDVTTGQKILLPETD
FTLPGRLPVTCSRFYASHLETVGLLGRGWRLNWETSLRDDDEHITLTGVQGRELRYPKTMLTPGHQIFDPEEQLYLSRLH
DGRYVLHYTDRSYYVFGDFDSDGMAYLLFMETPHRQRIVFGHEGGRLVRIASSSGHHLLLHRTQTPAGERLSRIELVQGG
TRGNLVEYRYDDNGQLTGVVNRAGTQVRQFAYENGLMTAHSNATGFTCRYRWQELDGAPRVTEHDTSDGEHYRFDYDFAA
GTTTVTGRQGETWQWWYDRETYITAHRTPGGGMYRFTYNEDHFPVNIELPGGRTVAYEYDIQNRVVKTTDPEGRVTQTQW
NGEFDEITRTALDDDAVWKTQYNAHGQPVQETDPEGRVTQYAYDEQGQMCSRTDAAGGTVVTAFDSRGQMTRYTDCSGRS
TGYDHDEDGNLTRVTDAEGKVVRISYNRLGLPETVNSPGKQQDRYTWNALGLMSSHRRITGSVESWRYTPRGLLAAHTDE
EKRETRWQYTPEGRVAALTNGNGAQYRFSHDADGRLVREVRPDGLSRTFILDDSGYLTAIQTTGTQGGVRRETQQRDALG
RLLRTENEHGQRTFSYNRLDQITAVTLTPTEAGQQQHRMQADTVRFEYDRSGWLTAEHAGNGSICYQRDALGNPTDITLP
DGQHLTHLYYGSGHLLQTALDGLTVSEYERDSLHRQIMRTQGQLATYSGYDDDGLLSWQRSLASGSAPVLPGQRPARQGC
VTSRDYYWNNHGEVGTIDDGLRGSVVYSYDRSGYLTGRSGQMYDHDRYYYDKAGNLLDNEGQGAVMSNRLPGCGRDRYGY
NEWGELTTRRDQQLEWNAQGQLTRVISGNTETHYGYDALGRRTRKATYGRHTGHTARSRTDFVWEGFRLLQENVQQQGWR
TYLYDAEQPYTPVASVTGRGESRQVWYYHTDVTGTPQEVTAADGTLVWAGYIRGFGENAADISNSGAYFHQPLRLPGQYF
DDETGLHYNLFRYYAPECGRFVSQDPIGLRGGLNLYQYAPNPLKYIDPLGLTATVGRWMGPAEYQQMLDTGTVVQSSTGT
THVAYPADIDAFGKQAKNGAMYVEFDVPEKSLVPTNEGWAKIVGPDSIEGRLAKRKGLPVPEMPTAENITVRGEKINGEV
EAKC*					

Nucleotide sequence: 4095 bp

						>T6CP011991 NC_016856:333236-337330 [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S] [PAAR]
ATGTATGAAGCAGCCCGTGTGGATGATCCTATCTACCACACCAGCGCGCTCGCCGGGTTTCTTATCGGCGCTATCATCGG
CATCGCCATTATCGCGCTTGCCGCCTTTGCCTTCTTTAGCTGCGGTTTTCTTGCCGGGCTGATTCTGGGTTTTATGGCCG
ATCAAATAGCCTCCGGGGTATTGCAACTGGGCGAGGCCATCGGGCGCTCCATCCACCACACGGCAGGAAAAATCCTCACC
GGTTCGGAGAATGTCAGCACCAACAGTCGCCCGGCGGCGCGCGCGGTACTGAGTACGGTGAAATGCGATAACCATATCGC
AGAAAAACGCATCGCCCAAGGGTCGGAAAATATCTACATCAACAGCCAGCCCGCCGCCCGTAAGGATGACCACACCGAAT
GCGACGCGGTGATTGAAGACGGTTCGCCGAATGTGTTTCTCGGCGGCGGCACACAGACGGTACTGGAAATCAGTTCTGAA
ATTCCGGACTGGCTGCGCAAGGTGGTGGATGTATTGTTTGTCGTGGCGAGTCTGCTCGGCGGGCTGGCCGGGGCGTGGCG
GCAGGCGGCAAAGCTGGGGACGAAATTTGGCACTAAATGTGCCGCTAAGTTTATCGGCGGGGAGCTTGTCGGGATGGCCG
TGGGTGAGGCTATCAGCGGGCTGTTCAGCAATCCGGTGGATGTGACCACCGGGCAGAAAATCCTGCTGCCGGAAACGGAC
TTCACCCTGCCCGGTCGCCTGCCGGTCACCTGCTCGCGTTTTTACGCCAGCCACCTGGAAACTGTGGGACTGTTGGGACG
GGGCTGGCGGCTGAACTGGGAAACCAGCCTGCGCGATGACGATGAACACATCACGCTGACCGGCGTACAGGGGCGGGAAC
TGCGTTACCCGAAAACGATGCTGACGCCCGGCCACCAGATATTTGACCCGGAAGAACAGTTATACCTCAGCCGCCTGCAT
GACGGGCGTTACGTGCTGCATTACACCGATCGCAGCTATTACGTATTTGGTGATTTTGACAGTGACGGCATGGCATACCT
GCTGTTTATGGAGACGCCGCACCGCCAGCGCATTGTCTTCGGGCACGAAGGAGGCAGACTGGTACGGATAGCCTCCAGCA
GCGGGCATCACCTGTTACTGCACCGCACACAGACCCCGGCAGGGGAGCGGCTGTCGCGAATTGAACTGGTGCAGGGCGGC
ACCCGTGGCAATCTGGTGGAGTACCGGTATGACGATAACGGTCAACTGACCGGCGTGGTGAACCGGGCGGGAACGCAGGT
GCGTCAGTTTGCTTATGAAAACGGGCTGATGACGGCGCACAGCAATGCGACGGGGTTCACCTGCCGCTACCGCTGGCAGG
AACTCGACGGCGCGCCGCGCGTGACGGAGCACGACACCAGTGACGGCGAACATTACCGCTTTGACTATGATTTTGCCGCA
GGCACCACCACCGTCACCGGCAGGCAGGGGGAGACATGGCAGTGGTGGTACGACAGGGAAACGTATATCACCGCGCACCG
GACGCCGGGCGGTGGAATGTACCGCTTCACGTACAACGAAGACCACTTCCCTGTCAACATTGAGCTGCCCGGCGGTCGCA
CGGTGGCGTATGAATATGACATCCAGAACCGGGTGGTGAAGACGACAGATCCGGAAGGCCGGGTGACGCAGACGCAGTGG
AACGGCGAGTTCGACGAAATCACGCGCACGGCGCTGGACGATGACGCTGTCTGGAAAACGCAGTACAACGCCCACGGCCA
GCCAGTGCAGGAGACGGACCCGGAAGGGCGGGTGACGCAGTACGCTTACGATGAACAGGGGCAGATGTGCAGCCGGACGG
ATGCGGCGGGCGGCACGGTGGTGACGGCGTTCGACAGCCGGGGGCAGATGACGCGGTACACCGACTGTTCAGGGCGCAGC
ACAGGATATGACCACGATGAGGACGGCAACCTGACGCGGGTGACGGACGCGGAAGGGAAGGTGGTACGCATCAGCTACAA
CCGACTTGGGTTGCCGGAGACGGTAAACTCACCGGGGAAACAGCAGGACAGGTATACCTGGAATGCGCTGGGGCTGATGA
GCAGCCACCGGCGCATCACGGGGAGCGTGGAGAGCTGGCGGTATACGCCGCGCGGTCTGCTGGCGGCGCACACGGATGAG
GAGAAGCGCGAGACGCGCTGGCAGTACACGCCGGAAGGCCGGGTGGCAGCGCTGACCAACGGCAACGGGGCGCAGTACCG
GTTCAGTCACGATGCGGACGGCAGGCTGGTGCGTGAGGTTCGCCCGGACGGACTGAGCCGTACTTTTATCCTGGACGACA
GCGGTTATCTGACGGCGATACAGACCACGGGCACGCAGGGCGGCGTGCGGCGGGAGACGCAGCAGCGGGATGCGCTGGGC
CGTCTGTTACGGACGGAGAATGAACACGGCCAGCGGACGTTCAGCTACAACCGGCTGGACCAGATAACGGCAGTGACGCT
CACGCCCACGGAGGCGGGGCAACAGCAGCACCGGATGCAGGCCGACACGGTGCGTTTTGAGTATGACCGCAGCGGCTGGC
TGACGGCGGAGCACGCGGGGAACGGTAGCATATGTTATCAGCGCGACGCGCTGGGCAACCCGACGGACATCACGCTGCCG
GACGGGCAGCACCTGACGCATCTGTATTACGGGAGCGGGCATCTGTTACAGACGGCGCTGGACGGCCTGACGGTGAGCGA
GTATGAGCGCGACAGCCTGCACCGTCAGATAATGCGCACGCAGGGGCAGCTTGCGACGTACAGCGGCTATGACGACGACG
GGCTGCTGAGCTGGCAGCGCAGTCTGGCGTCCGGCAGTGCCCCTGTTCTTCCTGGCCAGCGCCCGGCGCGGCAGGGCTGC
GTGACGTCGAGGGACTATTACTGGAACAACCACGGCGAGGTGGGCACGATTGACGACGGCCTGCGTGGCAGCGTGGTGTA
CAGCTATGACAGAAGCGGTTACCTGACCGGGCGCTCAGGTCAGATGTATGACCATGACCGTTATTATTACGATAAGGCGG
GCAACCTGCTGGATAACGAAGGGCAGGGAGCGGTGATGAGCAACCGGCTGCCGGGCTGTGGTCGTGACCGTTACGGCTAT
AACGAGTGGGGCGAGCTGACCACGCGGCGCGACCAGCAACTGGAGTGGAACGCGCAGGGGCAGCTGACGCGGGTCATCAG
CGGCAACACGGAGACGCACTACGGCTACGATGCGCTGGGGAGGCGAACCCGCAAGGCGACGTACGGGCGGCACACGGGCC
ATACGGCGCGGAGCCGGACGGACTTTGTGTGGGAGGGGTTCAGGCTGTTGCAGGAGAACGTGCAGCAGCAGGGCTGGCGG
ACCTATCTGTACGATGCGGAACAGCCGTACACGCCGGTGGCGAGCGTGACGGGGCGGGGAGAAAGCAGGCAGGTGTGGTA
TTACCACACGGATGTGACGGGCACGCCGCAGGAGGTGACGGCGGCGGACGGAACGCTGGTGTGGGCGGGGTATATCAGGG
GGTTTGGAGAGAATGCGGCGGACATCAGCAACAGCGGGGCGTACTTTCACCAGCCGCTGCGGCTGCCGGGGCAGTATTTT
GACGACGAGACAGGGCTGCATTACAATCTGTTCAGATATTATGCACCGGAGTGTGGACGGTTTGTCAGTCAGGATCCGAT
CGGGCTGAGGGGCGGGTTAAACCTTTATCAGTATGCGCCAAATCCTCTCAAATATATAGACCCACTTGGTTTAACCGCGA
CTGTTGGGCGATGGATGGGGCCTGCGGAATATCAGCAAATGCTTGATACTGGGACAGTAGTACAAAGTTCAACAGGGACA
ACTCATGTTGCCTACCCTGCTGATATAGATGCTTTTGGTAAGCAAGCAAAAAATGGTGCTATGTATGTTGAATTTGATGT
GCCTGAAAAATCATTAGTACCTACAAATGAAGGATGGGCAAAAATAGTAGGGCCAGATTCTATCGAAGGGCGATTAGCTA
AACGCAAAGGTTTGCCTGTTCCTGAAATGCCAACAGCAGAAAACATAACTGTAAGGGGCGAGAAAATTAATGGGGAAGTT
GAAGCAAAATGCTAA					