SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP011753
Component protein type	PAAR
T6SS ID (Type)	T6SS00344 (Type i2)
Strain	Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044
Replicon	chromosome
Sequence	Protein sequence (352 a.a.); Nucleotide sequence (1056 bp)
Reference	[1] Sarris PF, Zoumadakis C, Panopoulos NJ, et al. Distribution of the putative type VI secretion system core genes in Klebsiella spp. Infect Genet Evol. 2011 Jan;11(1):157-66. doi: 10.1016/j.meegid.2010.09.006. Epub 2010 Oct 4. PMID: 20932940 [2] Hsieh PF, Lu YR, Lin TL, et al. Klebsiella pneumoniae Type VI Secretion System Contributes to Bacterial Competition, Cell Invasion, Type-1 Fimbriae Expression, and In Vivo Colonization. J Infect Dis. 2019 Jan 29;219(4):637-647. doi: 10.1093/infdis/jiy534. PMID: 30202982

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	KP1_RS11305
Coordinate (Strand)	2323555..2324610 (+)
NCBI ID	WP_014907714.1
RefSeq	NC_012731
Uniprot ID	A0A652Q192_9ENTR, A0A332LZE1_KLEPN, A0A3F3BGA9_KLEPN
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Ntox44	PF15607.8	2.2e-31	196..303
PAAR_motif	PF05488.15	8.8e-09	11..74

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-351	MAAKGFYLVQGDRTTCGGRITTGAEDHTLFDKPVAREQDSVTCGKHAGLYKIAGGIDNDTIHGRRMAGTLDSCSTCPCKAKFIPSMMNDTYEKGGECITTLFSLDGLMASLQEPYKNLHPENKTERQCTHTDGAIKVAEYILSEIKANVRSDTAETIRYLIDEDFIIKRLTEWNKLPFYARLGPPPKTDLLAAMAIWYQTVKTGSIWDHKPKIRDFFSSVAVARPLPRKGKSSKSYYHKFKQHDYFYDVWSNIHYGYVGRSVGFSEAILLKGSTWEQNMTPGAMGDDTMDDITSMKIGFNLFYEHGKFAETLTADNILCVLETTPGAHLPHSRENHWCWNTDNPEKSDESF

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9671	-	-

Protein sequence: 352 a.a.

						>T6CP011753 NC_012731:2323555-2324610 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044] [PAAR]
MAAKGFYLVQGDRTTCGGRITTGAEDHTLFDKPVAREQDSVTCGKHAGLYKIAGGIDNDTIHGRRMAGTLDSCSTCPCKA
KFIPSMMNDTYEKGGECITTLFSLDGLMASLQEPYKNLHPENKTERQCTHTDGAIKVAEYILSEIKANVRSDTAETIRYL
IDEDFIIKRLTEWNKLPFYARLGPPPKTDLLAAMAIWYQTVKTGSIWDHKPKIRDFFSSVAVARPLPRKGKSSKSYYHKF
KQHDYFYDVWSNIHYGYVGRSVGFSEAILLKGSTWEQNMTPGAMGDDTMDDITSMKIGFNLFYEHGKFAETLTADNILCV
LETTPGAHLPHSRENHWCWNTDNPEKSDESF*					

Nucleotide sequence: 1056 bp

						>T6CP011753 NC_012731:2323555-2324610 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044] [PAAR]
ATGGCAGCAAAAGGTTTTTATCTGGTACAGGGAGACAGAACGACCTGCGGCGGAAGAATCACTACAGGCGCAGAAGATCA
CACTCTGTTTGATAAACCTGTTGCCCGGGAGCAGGACAGCGTCACTTGCGGAAAGCACGCAGGGCTATACAAGATTGCCG
GTGGCATCGACAACGATACCATTCATGGCCGGCGAATGGCCGGAACCCTCGACAGCTGTAGTACTTGCCCCTGTAAGGCG
AAGTTTATTCCATCAATGATGAATGATACTTATGAGAAAGGAGGTGAATGCATAACTACTTTATTTTCACTCGATGGGCT
TATGGCATCTTTACAGGAACCCTATAAGAATCTTCATCCAGAAAATAAGACTGAACGACAGTGTACTCACACAGATGGGG
CGATTAAAGTTGCAGAATATATACTAAGTGAAATAAAGGCGAATGTCAGAAGCGATACGGCAGAAACAATACGATATTTA
ATCGACGAAGATTTTATAATAAAAAGACTTACTGAATGGAATAAACTTCCCTTTTATGCAAGGCTGGGGCCGCCCCCTAA
AACAGATTTACTTGCTGCAATGGCTATTTGGTATCAGACCGTGAAAACAGGTTCAATCTGGGATCATAAACCGAAGATCC
GCGATTTTTTTTCATCCGTTGCAGTAGCACGACCACTTCCCAGGAAAGGAAAATCATCAAAGTCTTATTATCATAAGTTT
AAACAGCATGATTATTTTTATGATGTTTGGTCAAACATACATTATGGTTATGTTGGACGTAGTGTCGGTTTTTCTGAAGC
AATTCTCTTGAAAGGGTCCACTTGGGAACAAAACATGACGCCAGGTGCCATGGGGGATGATACCATGGATGACATTACTA
GCATGAAAATAGGGTTTAATCTATTTTATGAACATGGGAAATTTGCTGAGACGTTGACGGCAGATAATATTCTTTGCGTT
TTAGAAACAACACCTGGCGCGCACCTTCCTCACTCAAGAGAAAATCATTGGTGCTGGAATACAGATAATCCAGAAAAAAG
TGACGAATCATTTTGA					