SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP011446
Component protein type	TagL
T6SS ID (Type)	T6SS00053 (Type Unknown)
Strain	Photorhabdus luminescens subsp. laumondii TTO1
Replicon	chromosome
Sequence	Protein sequence (559 a.a.); Nucleotide sequence (1677 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PLU_RS20880
Coordinate (Strand)	4943908..4945584 (-)
NCBI ID	WP_011148325.1
RefSeq	NC_005126
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
OmpA	PF00691.22	1.9e-18	442..538

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-6	MSCVLK
Transmembrane helix	7-26	RALWLLAGVLAALLCLFFFP
Outside	27-30	LGNV
Transmembrane helix	31-50	SVALWLAVISMVAVMAWWWV
Inside	51-319	RRTTLEISHQASVYRLLAALPAASWHQPVVLTCGDSVATLFAGELVRFTPQGCFICVPKQSEMSTLVEAIIAARPDWCRQISVLLTLFPEQQWDHAVMAAKIREFRYQVDRVAQLTACHTPVLIASYLDGDISPWFELQADSATMTVWGEKHAAVGMNIWLTEGDAQQRVARLQQTIVAAAWQRWMVENILNECRSQELVSRPCYPIAMALTFLPQTPQADSLWTKWLVDRTTLPRISHSIAEAVNVSPFPDAMLRLLPHHVGHTPHRR
Transmembrane helix	320-342	AVIWGIGLLSGFTCLALASCAWN
Outside	343-558	NLRLFRQIRSDLQHYQVVGVKDSGAKVQAYHQLKRDATLLEKYQRDGEPVRLGLGLYLGDRLYTPLMTVINHAVLPVSSPPVLKSGKPLPQTLNLSSMSLFDVGQSRLKPEAAKVLVNALIEIKAKPEWMVVITGHTDSTGDAVKNRELSLARAKAVRDWILQTSDVSPTCFVVKGEGATQPIATNTTAVGRAVNRRVEISLMPPSVIACQRPENH

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9881	-	-

Protein sequence: 559 a.a.

						>T6CP011446 NC_005126:c4945584-4943908 [Photorhabdus laumondii subsp. laumondii TTO1] [TagL]
MSCVLKRALWLLAGVLAALLCLFFFPLGNVSVALWLAVISMVAVMAWWWVRRTTLEISHQASVYRLLAALPAASWHQPVV
LTCGDSVATLFAGELVRFTPQGCFICVPKQSEMSTLVEAIIAARPDWCRQISVLLTLFPEQQWDHAVMAAKIREFRYQVD
RVAQLTACHTPVLIASYLDGDISPWFELQADSATMTVWGEKHAAVGMNIWLTEGDAQQRVARLQQTIVAAAWQRWMVENI
LNECRSQELVSRPCYPIAMALTFLPQTPQADSLWTKWLVDRTTLPRISHSIAEAVNVSPFPDAMLRLLPHHVGHTPHRRA
VIWGIGLLSGFTCLALASCAWNNLRLFRQIRSDLQHYQVVGVKDSGAKVQAYHQLKRDATLLEKYQRDGEPVRLGLGLYL
GDRLYTPLMTVINHAVLPVSSPPVLKSGKPLPQTLNLSSMSLFDVGQSRLKPEAAKVLVNALIEIKAKPEWMVVITGHTD
STGDAVKNRELSLARAKAVRDWILQTSDVSPTCFVVKGEGATQPIATNTTAVGRAVNRRVEISLMPPSVIACQRPENH*					

Nucleotide sequence: 1677 bp

						>T6CP011446 NC_005126:c4945584-4943908 [Photorhabdus laumondii subsp. laumondii TTO1] [TagL]
TTGAGTTGCGTATTAAAACGAGCGTTGTGGCTTTTAGCGGGTGTTCTGGCGGCGCTGTTGTGTCTGTTTTTCTTCCCGTT
AGGGAATGTGTCAGTTGCATTGTGGTTGGCTGTGATCTCGATGGTAGCGGTGATGGCTTGGTGGTGGGTGAGGCGTACCA
CACTTGAAATATCCCATCAGGCCAGTGTTTACCGTTTGCTGGCGGCATTGCCCGCTGCTTCGTGGCACCAGCCTGTGGTG
CTAACCTGCGGAGATAGCGTGGCAACCCTGTTTGCGGGAGAACTTGTACGCTTCACGCCACAGGGCTGTTTTATCTGTGT
GCCGAAACAAAGTGAGATGAGTACGCTGGTAGAAGCGATTATTGCCGCCAGACCTGATTGGTGTAGGCAGATCAGCGTGT
TATTAACACTCTTTCCTGAACAGCAATGGGATCACGCGGTGATGGCGGCAAAGATCAGGGAATTTCGCTATCAGGTTGAT
CGTGTTGCTCAACTGACCGCTTGTCATACACCGGTATTGATCGCCAGCTATCTTGATGGCGATATCAGCCCGTGGTTCGA
GCTACAGGCAGATAGCGCGACAATGACGGTATGGGGAGAAAAACACGCCGCTGTTGGGATGAATATCTGGTTGACCGAAG
GAGATGCACAGCAACGCGTCGCACGTTTACAACAAACGATTGTGGCGGCGGCATGGCAACGCTGGATGGTAGAAAATATC
TTGAACGAATGCCGATCCCAGGAGCTGGTCAGTCGGCCTTGTTACCCGATAGCAATGGCACTGACGTTTTTGCCACAAAC
GCCACAGGCAGACAGCTTATGGACAAAATGGCTGGTCGATCGTACCACTTTACCCCGCATTAGCCACAGCATTGCTGAGG
CGGTGAACGTATCGCCATTTCCCGATGCCATGTTACGCCTGTTGCCACATCATGTTGGTCATACACCGCACCGACGAGCG
GTCATCTGGGGCATCGGCCTACTGAGCGGATTTACCTGTCTGGCGTTGGCCTCCTGTGCCTGGAACAACTTGCGTCTGTT
CCGCCAGATACGCAGTGATTTACAGCATTATCAGGTCGTTGGCGTGAAAGATAGTGGTGCAAAAGTTCAGGCTTACCATC
AACTTAAGCGTGATGCGACGTTGCTGGAAAAATATCAACGTGATGGCGAACCGGTGCGTTTAGGGTTAGGGCTCTATTTA
GGTGATCGCCTTTATACGCCACTGATGACTGTGATCAACCATGCGGTGTTACCGGTATCTTCGCCTCCTGTACTGAAGAG
TGGCAAGCCGTTGCCACAGACATTGAACCTGAGCAGCATGTCACTGTTTGACGTTGGACAGTCACGTCTTAAGCCAGAAG
CAGCCAAAGTGTTGGTGAACGCATTGATCGAGATTAAAGCTAAACCGGAGTGGATGGTGGTGATAACGGGTCATACTGAT
TCAACCGGTGATGCCGTAAAAAACCGCGAGCTATCACTAGCGCGGGCAAAAGCAGTACGAGATTGGATATTACAAACCAG
TGATGTATCGCCGACTTGCTTTGTCGTAAAAGGTGAAGGAGCAACGCAGCCGATTGCCACCAATACAACCGCAGTGGGGC
GTGCTGTTAATCGTAGGGTTGAAATCAGTCTGATGCCGCCGAGCGTGATCGCCTGCCAACGACCAGAAAATCACTAG					