SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP011384
Component protein type	FHA
T6SS ID (Type)	T6SS00161 (Type i3)
Strain	Azoarcus olearius
Replicon	chromosome
Sequence	Protein sequence (612 a.a.); Nucleotide sequence (1836 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Shidore T, Dinse T, Öhrlein J, et al. Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte Azoarcus sp. strain BH72. Environ Microbiol. 2012 Oct;14(10):2775-87. doi: 10.1111/j.1462-2920.2012.02777.x. Epub 2012 May 23. PMID: 22616609

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AZO_RS19490
Coordinate (Strand)	4257210..4259045 (+)
NCBI ID	WP_011767606.1
RefSeq	NC_008702
Uniprot ID	A1KCE4_AZOSB
KEGG ID	azo:azo3884
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
FHA	PF00498.28	3.9e-11	28..93

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-611	VLRISVIQVNGFPPATPLAGEFDERGGTIGRDDSNALTLPDPGRHISRMQAQVQYDGSRYVLTDHGGNPTHLNGRPLGKGASATLRDGDEIGIADYTLRVEIVRTVSGFGNETLPNIEQRGDPLGLDLDLALPPDLPAGLGAPAAAAPPPRAAALDDDPFAVFAAATPTPPAPPAAAPPAASVSDDPFAAFAPARPAPPPAPASSRAGDPLGIGALAQPAEASSLDALFGLDGASTGGDPFAGSPLGNPAHAPAPFDGGGEDPLALLGGMAATPSSAPVRNDSPLLNDAFAPPRLIDDSPPRPLAPAPASAAAPAPQRSPAAPQGGVVSWASTPAAPAAPAAPAVPAAGDDGREAPTRIVPAFPPPEAPRANVGIERAPLTPAPAPAPSPVPPASAPAAAAQPAAASAGSSADADALLAAFARGIGLPRLAPAGGLTPELMEHIGRMLREAVQGTVELLVARAATKREVRADVTMIVSKNNNPLKFSPDVDFALMQLLLPQGSGFMKPDEAMQDAYDDLRAHQLGFMAGMRAALASILGRFTPAELEARLTTKSFLDNVLPANRKAKLWDLYEQRYGDISREAEDDFHSLFGREFLKAYEAQIDQLQRERG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9084	-	-

Protein sequence: 612 a.a.

						>T6CP011384 NC_008702:4257210-4259045 [Azoarcus olearius] [FHA]
VLRISVIQVNGFPPATPLAGEFDERGGTIGRDDSNALTLPDPGRHISRMQAQVQYDGSRYVLTDHGGNPTHLNGRPLGKG
ASATLRDGDEIGIADYTLRVEIVRTVSGFGNETLPNIEQRGDPLGLDLDLALPPDLPAGLGAPAAAAPPPRAAALDDDPF
AVFAAATPTPPAPPAAAPPAASVSDDPFAAFAPARPAPPPAPASSRAGDPLGIGALAQPAEASSLDALFGLDGASTGGDP
FAGSPLGNPAHAPAPFDGGGEDPLALLGGMAATPSSAPVRNDSPLLNDAFAPPRLIDDSPPRPLAPAPASAAAPAPQRSP
AAPQGGVVSWASTPAAPAAPAAPAVPAAGDDGREAPTRIVPAFPPPEAPRANVGIERAPLTPAPAPAPSPVPPASAPAAA
AQPAAASAGSSADADALLAAFARGIGLPRLAPAGGLTPELMEHIGRMLREAVQGTVELLVARAATKREVRADVTMIVSKN
NNPLKFSPDVDFALMQLLLPQGSGFMKPDEAMQDAYDDLRAHQLGFMAGMRAALASILGRFTPAELEARLTTKSFLDNVL
PANRKAKLWDLYEQRYGDISREAEDDFHSLFGREFLKAYEAQIDQLQRERG*					

Nucleotide sequence: 1836 bp

						>T6CP011384 NC_008702:4257210-4259045 [Azoarcus olearius] [FHA]
GTGCTACGCATCAGTGTCATCCAGGTCAACGGCTTTCCGCCCGCCACCCCGCTTGCCGGCGAATTCGACGAGCGCGGCGG
CACCATCGGTCGCGACGACAGCAACGCGCTGACCCTGCCCGACCCCGGCCGCCACATCTCGCGCATGCAGGCCCAGGTCC
AGTACGACGGCAGCCGCTATGTGCTGACCGACCACGGCGGCAACCCCACCCATCTCAACGGCCGCCCGCTGGGCAAGGGC
GCCAGTGCCACGCTGCGCGACGGCGACGAGATCGGCATCGCCGACTACACGCTGCGGGTCGAGATCGTGCGCACCGTATC
CGGCTTCGGTAACGAAACGCTGCCCAACATCGAACAGCGCGGCGACCCGCTCGGGCTGGATCTCGATCTCGCGCTGCCGC
CCGATCTTCCGGCGGGCCTCGGCGCCCCGGCCGCAGCCGCGCCGCCGCCGCGCGCCGCCGCGCTCGACGACGATCCGTTC
GCGGTGTTCGCCGCCGCCACGCCGACGCCGCCCGCGCCGCCTGCTGCCGCTCCGCCCGCGGCGTCGGTGTCCGACGACCC
GTTCGCGGCATTCGCGCCCGCGCGGCCGGCCCCGCCCCCCGCGCCCGCGTCCAGCCGCGCGGGCGATCCGCTCGGCATCG
GTGCGCTGGCGCAGCCCGCCGAAGCGAGTTCGCTCGACGCGCTGTTCGGCCTCGACGGCGCCAGCACCGGCGGCGACCCC
TTCGCCGGCTCGCCGCTCGGCAACCCGGCCCATGCGCCCGCCCCGTTCGACGGGGGCGGCGAAGACCCGCTGGCGCTGCT
CGGCGGCATGGCGGCAACGCCCAGCAGCGCCCCGGTGCGCAACGATTCGCCGCTGCTCAACGACGCCTTCGCCCCGCCGC
GCCTGATCGACGACAGCCCACCGCGGCCGCTTGCCCCCGCCCCAGCGTCCGCCGCCGCACCGGCACCGCAGCGCAGCCCC
GCTGCGCCGCAAGGCGGCGTGGTGTCGTGGGCCAGCACGCCCGCCGCCCCCGCCGCACCGGCAGCGCCGGCAGTGCCGGC
CGCCGGGGACGACGGACGCGAGGCGCCGACCCGCATCGTGCCCGCATTCCCCCCGCCGGAGGCCCCGCGCGCCAACGTCG
GCATCGAACGCGCGCCGCTGACGCCCGCCCCGGCGCCGGCGCCCTCCCCCGTGCCGCCGGCGTCTGCACCAGCCGCCGCG
GCGCAACCCGCGGCCGCCAGCGCCGGCTCCAGTGCCGACGCCGACGCGCTGCTGGCCGCCTTTGCCCGCGGCATCGGCCT
GCCGCGGCTGGCCCCGGCGGGCGGGCTGACCCCCGAACTGATGGAGCACATCGGCCGCATGCTGCGCGAGGCGGTGCAGG
GCACGGTGGAACTGCTGGTCGCGCGCGCGGCCACCAAGCGCGAGGTGCGCGCGGACGTGACCATGATCGTCAGCAAGAAC
AACAACCCGCTGAAGTTCTCGCCCGACGTCGATTTCGCGCTGATGCAGCTGCTGCTGCCGCAGGGCAGCGGCTTCATGAA
GCCGGACGAGGCGATGCAGGACGCCTACGACGATCTGCGCGCCCATCAGCTCGGCTTCATGGCCGGCATGCGTGCGGCGC
TGGCGAGCATCCTCGGCCGCTTCACCCCGGCCGAGCTGGAAGCCCGCCTGACCACCAAGAGCTTCCTCGACAACGTGCTG
CCGGCCAACCGCAAGGCCAAGCTGTGGGACCTCTACGAGCAACGCTATGGCGACATCTCGCGCGAGGCCGAGGACGACTT
CCATTCGCTGTTCGGGCGCGAGTTCCTGAAGGCCTACGAGGCCCAGATCGACCAGTTGCAGCGCGAACGCGGCTGA					