Detailed information of component protein


Summary

Component ID T6CP011358
Component protein type TagL
T6SS ID (Type) T6SS00463 experimental (Type i2)
Strain Escherichia coli 042
Replicon chromosome
Sequence Protein sequence (577 a.a.); Nucleotide sequence (1731 bp)
Reference
[1] Durand E, Zoued A, Spinelli S, et al. Structural characterization and oligomerization of the TssL protein, a component shared by bacterial type VI and type IVb secretion systems. J Biol Chem. 2012 Apr 20;287(17):14157-68. doi: 10.1074/jbc.M111.338731. Epub 2012 Feb 27. PMID: 22371492
[2] Felisberto-Rodrigues C, Durand E, Aschtgen MS, et al. Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathog. 2011 Nov;7(11):e1002386. doi: 10.1371/journal.ppat.1002386. Epub 2011 Nov 10. PMID: 22102820
[3] Dudley EG, Thomson NR, Parkhill J, et al. Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli. Mol Microbiol. 2006 Sep;61(5):1267-82. PMID: 16925558
[4] Morin N, Santiago AE, Ernst RK, et al. Characterization of the AggR regulon in enteroaggregative Escherichia coli. Infect Immun. 2013 Jan;81(1):122-32. doi: 10.1128/IAI.00676-12. Epub 2012 Oct 22. PMID: 23090962
[5] Zoued A, Durand E, Bebeacua C, et al. TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the type VI secretion system. J Biol Chem. 2013 Sep 20;288(38):27031-27041. doi: 10.1074/jbc.M113.499772. Epub 2013 Aug 6. PMID: 23921384
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) EC042_RS24190
Coordinate (Strand) 4856944..4858674 (+)
NCBI ID WP_000558483.1
RefSeq NC_017626
Uniprot ID D3GUV9_ECO44, A0A377L9R2_ECOLX
KEGG ID elo:EC042_4528
PDB ID 7BBA, 5M38
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
OmpA PF00691.22 2.4e-15 448..544
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MHVRSR
Transmembrane helix 7-29 LWLTILSLFLTLWLISGFLPLSA
Outside 30-32 VNQ
Transmembrane helix 33-51 IILTLVSVVAAGLVLWYQW
Inside 52-329 RCLSSRRDIPEKAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGWYLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQCRQWLNTTPPVLVTLMVSEPSPQNTSLNKHSTHWFTLTPDLPGMHIRLTEAGAIPAEEWLQACDAASRLRCVSELLWLNAMLSWYNRVTDALTAICRQEHFYFRPVAVGFCLTSVAARPGNLWQQQLTSLTALPPPAGVSSQVLPLPDILLSGIPRHRGLSHWHRLCRQ
Transmembrane helix 330-347 TGAIVGVFLLLAMLASFM
Outside 348-576 NNQRLVRSVGEHLTAWHRMRGYSSESKTTALQRLQADSQLLNDWQQRGVPQRYGLGLYQGMWLIPPVERATGEWVAPRPPRPVIQKVTQTPKTVRLNSLALFDAGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPLRNQALSLKRAEAVRDWMRDTGDIPQSCFAVQGYGESRPVAPNDTAEGRARNRRVEISLVPQADACRLPDVSPAPEEGASENITE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9945 - -


  Protein sequence: 577 a.a.    

>T6CP011358 NC_017626:4856944-4858674 [Escherichia coli 042] [TagL]
MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAGLVLWYQWRCLSSRRDIPEKAGDDGLPPEYFQGHVLL
VAGNSDAWFEPGQAYRETASGWYLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
CRQWLNTTPPVLVTLMVSEPSPQNTSLNKHSTHWFTLTPDLPGMHIRLTEAGAIPAEEWLQACDAASRLRCVSELLWLNA
MLSWYNRVTDALTAICRQEHFYFRPVAVGFCLTSVAARPGNLWQQQLTSLTALPPPAGVSSQVLPLPDILLSGIPRHRGL
SHWHRLCRQTGAIVGVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKTTALQRLQADSQLLNDWQQRGVPQRY
GLGLYQGMWLIPPVERATGEWVAPRPPRPVIQKVTQTPKTVRLNSLALFDAGKWTLKPGATKWLVNALVDIKAKAGWLIV
VSGHTDNTGDPLRNQALSLKRAEAVRDWMRDTGDIPQSCFAVQGYGESRPVAPNDTAEGRARNRRVEISLVPQADACRLP
DVSPAPEEGASENITE*

  Nucleotide sequence: 1731 bp    

>T6CP011358 NC_017626:4856944-4858674 [Escherichia coli 042] [TagL]
ATGCACGTACGTTCCCGTCTCTGGCTGACAATACTGAGTCTGTTCCTGACACTGTGGCTGATTTCGGGATTCCTGCCGCT
GTCTGCAGTAAACCAGATAATACTGACCCTGGTTTCAGTTGTGGCGGCGGGGCTTGTGCTGTGGTACCAGTGGCGTTGTC
TGTCTTCCCGTCGTGATATCCCGGAGAAGGCCGGCGATGACGGTCTGCCGCCTGAGTATTTTCAGGGACATGTGCTGCTT
GTTGCCGGAAACTCAGATGCGTGGTTCGAACCCGGTCAGGCATACCGGGAAACAGCATCGGGATGGTATCTTCGTGTGGA
TACGCCGGAGCAGATGAGAATACTGGCAGAACGGTTGTCGTACAGCCGTCCGGCCCTGTGTGGCCAGGTCGTGGTCATGC
TGGGACTTTTACCGGAGCAGCATACCTCAGATGAAGCTTTCCTGTCGTGGTTGCGGAACTGGCAGCGTGCAGTCATGCAG
TGCCGGCAGTGGCTGAATACCACGCCACCGGTGCTGGTTACATTGATGGTTTCAGAGCCGTCACCACAAAACACATCCTT
AAATAAGCACTCTACACACTGGTTTACACTGACACCGGACCTGCCGGGAATGCATATACGCCTGACGGAAGCCGGAGCAA
TTCCGGCAGAGGAATGGCTGCAGGCGTGTGATGCAGCGTCCAGGCTCCGGTGCGTCAGTGAACTGTTATGGCTGAATGCC
ATGTTGTCCTGGTACAACCGGGTTACTGATGCCCTGACAGCTATTTGCCGGCAGGAGCACTTTTATTTCCGGCCGGTTGC
CGTTGGATTCTGTCTGACGTCGGTTGCGGCCCGCCCCGGTAATCTCTGGCAACAACAACTGACGTCCCTCACTGCATTGC
CACCGCCTGCCGGCGTGTCATCACAGGTATTGCCGTTGCCGGATATTCTGCTGTCCGGTATTCCCCGTCATCGTGGACTC
AGTCACTGGCACAGACTTTGTCGCCAGACGGGGGCGATTGTCGGTGTTTTTCTTCTGCTGGCTATGCTGGCGTCTTTTAT
GAATAACCAGCGGCTGGTTCGCAGCGTGGGGGAACACCTGACGGCCTGGCACCGGATGCGCGGATATTCATCTGAATCGA
AAACAACAGCACTGCAACGTCTTCAGGCTGACAGCCAGTTACTGAACGACTGGCAGCAACGTGGTGTGCCACAGCGTTAC
GGACTGGGATTGTATCAGGGAATGTGGCTGATACCGCCGGTTGAGCGTGCGACTGGTGAGTGGGTTGCACCACGGCCTCC
CCGTCCGGTGATACAGAAAGTTACTCAGACGCCGAAAACGGTTCGCCTTAACAGCCTGGCACTGTTTGATGCCGGTAAAT
GGACGCTGAAGCCGGGCGCCACAAAATGGCTGGTGAATGCCCTGGTGGATATTAAGGCAAAAGCAGGCTGGCTGATTGTG
GTCAGTGGTCACACGGATAATACCGGTGACCCGCTGCGCAACCAGGCCTTGTCCCTGAAGCGTGCAGAGGCCGTTCGCGA
CTGGATGCGGGATACCGGCGATATTCCCCAAAGCTGTTTTGCCGTACAGGGATACGGTGAAAGCCGCCCTGTGGCCCCGA
ATGACACGGCGGAAGGCCGTGCGCGTAACCGCCGTGTTGAAATCAGTCTGGTACCGCAGGCCGATGCCTGTCGTCTGCCG
GACGTTTCCCCTGCGCCGGAAGAGGGCGCATCAGAAAACATAACGGAGTAA