SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP011291
Component protein type	TssO
T6SS ID (Type)	T6SS00926 (Type iii)
Strain	Bacteroides fragilis 638R
Replicon	chromosome
Sequence	Protein sequence (168 a.a.); Nucleotide sequence (504 bp)
Reference	[1] Chatzidaki-Livanis M, Geva-Zatorsky N, Comstock LE. Bacteroides fragilis type VI secretion systems use novel effector and immunity proteins to antagonize human gut Bacteroidales species. Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):3627-32. doi: 10.1073/pnas.1522510113. Epub 2016 Mar 7. PMID: 26951680

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BF638R_RS09270
Coordinate (Strand)	2331489..2331992 (-)
NCBI ID	WP_005787050.1
RefSeq	NC_016776
Uniprot ID	A0A015XI10_BACFG, A0A016CPR5_BACFG, A0A015V650_BACFG, A0A015X3T8_BACFG, A0A017NDR3_BACFG, A0A016EEE2_BACFG, Q5LDV2_BACFN, A0A081TTN3_BACFG, E1WVT1_BACF6, A0A0E2ANW2_BACFG
KEGG ID	bfb:VU15_08280, bfg:BF638R_1974, bfs:BF9343_1922
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
TssO	PF17561.4	2.9e-07	5..149

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-12	MKAKNSEKIIRG
Transmembrane helix	13-35	YLEFAGGLLISTALSMALLTGFI
Outside	36-167	HTNGSEYKLMESKTQEYDKIYARQIALVDKVDSLYNYLVLMGSNDRLNQVVLQKVISTRKMELIEELQIMDSKDVLLYKKLASQINVFLDTKEAIRKAVIEESLVRKDLMRCIQDNKQATRKLTLGNISVEK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8539	-	-

Protein sequence: 168 a.a.

						>T6CP011291 NC_016776:c2331992-2331489 [Bacteroides fragilis 638R] [TssO]
MKAKNSEKIIRGYLEFAGGLLISTALSMALLTGFIHTNGSEYKLMESKTQEYDKIYARQIALVDKVDSLYNYLVLMGSND
RLNQVVLQKVISTRKMELIEELQIMDSKDVLLYKKLASQINVFLDTKEAIRKAVIEESLVRKDLMRCIQDNKQATRKLTL
GNISVEK*					

Nucleotide sequence: 504 bp

						>T6CP011291 NC_016776:c2331992-2331489 [Bacteroides fragilis 638R] [TssO]
ATGAAAGCAAAGAACAGTGAGAAAATCATCCGTGGATATTTGGAGTTTGCCGGCGGACTGTTAATCAGTACGGCTTTGAG
CATGGCACTACTCACAGGCTTTATCCATACGAATGGAAGCGAATATAAACTGATGGAATCCAAAACGCAAGAATACGATA
AGATTTACGCCAGACAGATTGCCCTGGTGGATAAGGTGGATTCGCTGTATAACTACCTGGTGCTGATGGGCAGTAACGAC
CGGCTTAATCAGGTGGTGCTCCAAAAAGTTATCAGTACACGCAAGATGGAACTTATCGAGGAACTGCAAATAATGGATAG
TAAGGATGTACTTCTGTACAAAAAACTGGCTTCGCAAATCAACGTGTTTCTGGATACCAAAGAAGCCATACGTAAAGCGG
TTATTGAAGAAAGCCTTGTGAGAAAAGACCTGATGCGGTGCATTCAGGACAATAAACAGGCTACCCGAAAGCTGACATTG
GGAAACATTAGTGTAGAAAAGTAA					