SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP011290
Component protein type	TssO
T6SS ID (Type)	T6SS00926 (Type iii)
Strain	Bacteroides fragilis 638R
Replicon	chromosome
Sequence	Protein sequence (176 a.a.); Nucleotide sequence (528 bp)
Reference	[1] Chatzidaki-Livanis M, Geva-Zatorsky N, Comstock LE. Bacteroides fragilis type VI secretion systems use novel effector and immunity proteins to antagonize human gut Bacteroidales species. Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):3627-32. doi: 10.1073/pnas.1522510113. Epub 2016 Mar 7. PMID: 26951680

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BF638R_RS09265
Coordinate (Strand)	2330949..2331476 (-)
NCBI ID	WP_008768464.1
RefSeq	NC_016776
Uniprot ID	A0A015Z1N0_BACFG, Q64UZ5_BACFR, A0A5M5VWX8_BACFG, E1WVH2_BACF6
KEGG ID	bfr:BF1936, bfg:BF638R_1973
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
TssO	PF17561.4	2.6e-52	13..160

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-18	MSITPKNKSSKAVSERER
Transmembrane helix	19-41	FIGFVYVLTLFIVITGACGFILF
Outside	42-175	KYAGTRHIFSNKIMVIKKMERQKEFQNIQSVQIVSADTLFSRIEQFEPGVNASYEENDIKFLINDLAKQWEKNSFDKRNKMFWHLASVYEMWFADKKELWSKQDNIVKFRKNLEECEVGLQKKEGELKNKGGKP

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8560	-	-

Protein sequence: 176 a.a.

						>T6CP011290 NC_016776:c2331476-2330949 [Bacteroides fragilis 638R] [TssO]
MSITPKNKSSKAVSERERFIGFVYVLTLFIVITGACGFILFKYAGTRHIFSNKIMVIKKMERQKEFQNIQSVQIVSADTL
FSRIEQFEPGVNASYEENDIKFLINDLAKQWEKNSFDKRNKMFWHLASVYEMWFADKKELWSKQDNIVKFRKNLEECEVG
LQKKEGELKNKGGKP*					

Nucleotide sequence: 528 bp

						>T6CP011290 NC_016776:c2331476-2330949 [Bacteroides fragilis 638R] [TssO]
ATGAGCATAACCCCTAAAAATAAAAGCAGCAAAGCCGTTAGCGAGCGTGAACGCTTTATCGGATTTGTATATGTGCTTAC
CCTGTTTATTGTTATCACAGGAGCCTGTGGATTTATTCTTTTCAAGTATGCAGGGACACGCCACATCTTCTCCAATAAAA
TAATGGTCATTAAAAAGATGGAGCGGCAAAAGGAATTTCAAAACATACAATCTGTACAGATTGTGAGCGCAGATACCCTA
TTTTCACGTATTGAACAGTTTGAGCCGGGCGTCAATGCCTCTTATGAGGAAAATGATATCAAGTTCCTGATAAACGACCT
TGCCAAACAATGGGAGAAAAACAGCTTTGACAAGCGCAATAAGATGTTCTGGCATCTCGCTTCGGTATATGAAATGTGGT
TTGCCGATAAGAAAGAACTATGGAGCAAGCAGGATAACATAGTGAAGTTCAGGAAAAATCTGGAGGAGTGCGAAGTCGGA
CTCCAGAAGAAAGAAGGCGAACTTAAAAATAAAGGAGGCAAGCCATGA					