SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP011289
Component protein type	TssP
T6SS ID (Type)	T6SS00926 (Type iii)
Strain	Bacteroides fragilis 638R
Replicon	chromosome
Sequence	Protein sequence (298 a.a.); Nucleotide sequence (894 bp)
Reference	[1] Chatzidaki-Livanis M, Geva-Zatorsky N, Comstock LE. Bacteroides fragilis type VI secretion systems use novel effector and immunity proteins to antagonize human gut Bacteroidales species. Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):3627-32. doi: 10.1073/pnas.1522510113. Epub 2016 Mar 7. PMID: 26951680

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BF638R_RS09260
Coordinate (Strand)	2330059..2330952 (-)
NCBI ID	WP_008768463.1
RefSeq	NC_016776
Uniprot ID	UPI0001BD8FA5
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
PKD_4	PF18911.2	1.1e-07	41..96
PKD	PF00801.22	1e-05	143..180

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-9	MMNKFLNTK
Transmembrane helix	10-27	VLYIFISVIILIMVFLLV
Inside	28-297	MRACSQKQPIQAVVTPSDPVVGEEIFFSDSTSGARIWYWEFGNNETSTQRSGHHRFKQKGVYKIRLTVNGNLERYFDVRVKEKTNTEDLHLVHIIAPKEAIQGENIIFRGEGHDEQWRWEFGETGMIDSREKTALYAYTEPGEYEVLLNTENTRYPIRHRINILPYYSENDSTDVMVLIGLDIKEKLQNIADGKPFNVNYNYVVDKYFNNNPNTLVIINNNKYNDFYSYCQGLHHIGRKETIIQNVIVETEDEESGYITQITVMQIEKKK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.5278	pos: 30-31. MRA-CS.	MMNKFLNTKVLYIFISVIILIMVFLLVMRA

Protein sequence: 298 a.a.

						>T6CP011289 NC_016776:c2330952-2330059 [Bacteroides fragilis 638R] [TssP]
MMNKFLNTKVLYIFISVIILIMVFLLVMRACSQKQPIQAVVTPSDPVVGEEIFFSDSTSGARIWYWEFGNNETSTQRSGH
HRFKQKGVYKIRLTVNGNLERYFDVRVKEKTNTEDLHLVHIIAPKEAIQGENIIFRGEGHDEQWRWEFGETGMIDSREKT
ALYAYTEPGEYEVLLNTENTRYPIRHRINILPYYSENDSTDVMVLIGLDIKEKLQNIADGKPFNVNYNYVVDKYFNNNPN
TLVIINNNKYNDFYSYCQGLHHIGRKETIIQNVIVETEDEESGYITQITVMQIEKKK*					

Nucleotide sequence: 894 bp

						>T6CP011289 NC_016776:c2330952-2330059 [Bacteroides fragilis 638R] [TssP]
ATGATGAATAAATTCCTAAATACGAAGGTTCTCTATATCTTTATCTCCGTTATCATACTTATTATGGTTTTTCTGTTGGT
AATGAGAGCTTGCTCGCAGAAGCAGCCGATTCAGGCTGTTGTAACTCCGTCTGACCCTGTCGTGGGGGAAGAAATATTTT
TTTCCGATTCCACGTCGGGAGCGAGAATCTGGTACTGGGAGTTCGGAAACAACGAGACATCCACACAACGCAGCGGACAC
CATCGGTTCAAGCAAAAAGGGGTGTACAAAATACGACTGACCGTCAACGGAAATCTGGAACGCTACTTTGATGTGAGGGT
AAAAGAGAAGACCAATACGGAAGACCTGCATCTGGTTCATATCATAGCTCCCAAGGAAGCCATTCAGGGAGAAAATATCA
TCTTCCGTGGCGAAGGACACGACGAACAATGGCGCTGGGAGTTCGGAGAGACGGGGATGATTGATTCTCGTGAAAAAACG
GCTCTTTATGCTTATACCGAACCCGGAGAGTACGAAGTATTGCTTAATACGGAGAATACCCGGTATCCCATCAGACACCG
GATAAACATTCTGCCCTACTATTCGGAGAATGATTCTACCGATGTAATGGTGCTCATCGGTTTAGACATCAAAGAGAAGT
TACAGAACATTGCAGACGGTAAACCCTTTAATGTAAACTACAACTATGTCGTGGACAAGTATTTTAATAATAACCCGAAC
ACGCTGGTTATTATCAACAACAATAAATATAACGACTTTTATTCTTATTGCCAAGGACTGCACCATATCGGCAGAAAAGA
AACGATTATCCAGAATGTCATCGTAGAGACGGAGGATGAAGAGAGCGGATATATTACCCAAATAACGGTTATGCAAATCG
AAAAAAAGAAATGA					