SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP011099
Component protein type	TssG
T6SS ID (Type)	T6SS00910 (Type iii)
Strain	Flavobacterium johnsoniae UW101
Replicon	chromosome
Sequence	Protein sequence (315 a.a.); Nucleotide sequence (945 bp)
Reference	[1] Russell AB, Wexler AG, Harding BN, et al. A type VI secretion-related pathway in Bacteroidetes mediates interbacterial antagonism. Cell Host Microbe. 2014 Aug 13;16(2):227-236. doi: 10.1016/j.chom.2014.07.007. Epub 2014 Jul 25. PMID: 25070807

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	FJOH_RS16995
Coordinate (Strand)	3917665..3918609 (-)
NCBI ID	WP_012025263.1
RefSeq	NC_009441
Uniprot ID	A5FES0_FLAJ1
KEGG ID	fjo:Fjoh_3278
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	6.3e-06	219..297

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-314	MERKSKKSLEDLAYEIENISYDIRAEVIANELLENKAVVQDEITISNHGQFSRAYRSDILGAAIQDDNYDKQEYLNIRLSRDSMYDALPEGFVHSLTENNSDKSVKQMIREHKHQKKQEAEARVFFTPFENEIFHYRTKIESVERNFLYKLNGSKPLDFFYDFWGLSHIYPEILVSKFIQLLPYAYKIVGDIDLACNCLSSIIEEKVTYTTTSSKEYSEEAEQIKLGENRLGVDFITGKDYMDYSMNLRIEIGPIMNKPYQHYLKNGDIKIFIDCFCEHFFPMEVEVKTVLLINEETEEFNFSKEPVLGYTTRI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9952	-	-

Protein sequence: 315 a.a.

						>T6CP011099 NC_009441:c3918609-3917665 [Flavobacterium johnsoniae UW101] [TssG]
MERKSKKSLEDLAYEIENISYDIRAEVIANELLENKAVVQDEITISNHGQFSRAYRSDILGAAIQDDNYDKQEYLNIRLS
RDSMYDALPEGFVHSLTENNSDKSVKQMIREHKHQKKQEAEARVFFTPFENEIFHYRTKIESVERNFLYKLNGSKPLDFF
YDFWGLSHIYPEILVSKFIQLLPYAYKIVGDIDLACNCLSSIIEEKVTYTTTSSKEYSEEAEQIKLGENRLGVDFITGKD
YMDYSMNLRIEIGPIMNKPYQHYLKNGDIKIFIDCFCEHFFPMEVEVKTVLLINEETEEFNFSKEPVLGYTTRI*					

Nucleotide sequence: 945 bp

						>T6CP011099 NC_009441:c3918609-3917665 [Flavobacterium johnsoniae UW101] [TssG]
ATGGAAAGAAAAAGTAAAAAAAGCCTTGAAGATCTTGCGTACGAAATTGAAAATATTTCGTATGATATTAGAGCGGAAGT
GATTGCTAATGAGCTTCTTGAAAACAAAGCTGTGGTTCAGGATGAAATTACGATTTCGAATCACGGACAGTTTTCACGTG
CCTACAGAAGCGATATTCTGGGAGCGGCAATTCAGGATGATAATTACGACAAACAAGAATATCTAAATATACGCTTATCT
CGAGACAGTATGTATGACGCACTTCCGGAAGGTTTTGTGCATAGTCTGACCGAAAATAACTCTGATAAATCTGTAAAACA
GATGATAAGGGAGCATAAACACCAGAAAAAGCAAGAGGCTGAAGCACGTGTTTTTTTTACTCCTTTTGAAAACGAAATTT
TTCATTATCGGACTAAAATTGAAAGTGTTGAAAGGAATTTTTTGTATAAGCTGAACGGGAGTAAACCGTTAGATTTTTTC
TATGATTTTTGGGGTTTATCACACATTTATCCAGAGATTTTAGTTTCAAAATTTATTCAGTTACTGCCGTATGCCTACAA
GATAGTTGGAGATATTGATCTTGCCTGTAATTGTTTATCAAGCATTATAGAAGAAAAAGTAACCTATACAACAACAAGTT
CTAAAGAATACAGCGAAGAAGCTGAGCAGATAAAGCTGGGTGAAAACAGGTTAGGAGTCGATTTTATTACCGGAAAAGAT
TATATGGATTATTCTATGAATTTAAGGATAGAAATAGGTCCTATAATGAACAAACCTTATCAGCATTACCTTAAAAACGG
CGATATAAAAATATTTATAGATTGTTTTTGCGAACATTTTTTTCCAATGGAAGTAGAAGTAAAAACAGTGTTATTGATTA
ATGAAGAAACAGAAGAGTTTAATTTTAGTAAAGAACCTGTGTTAGGGTATACAACACGAATTTAG					