Detailed information of component protein


Summary

Component ID T6CP011098
Component protein type TssN
T6SS ID (Type) T6SS00910 experimental (Type iii)
Strain Flavobacterium johnsoniae UW101
Replicon chromosome
Sequence Protein sequence (324 a.a.); Nucleotide sequence (972 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) FJOH_RS16990
Coordinate (Strand) 3916691..3917662 (-)
NCBI ID WP_012025262.1
RefSeq NC_009441
Uniprot ID A5FER9_FLAJ1
KEGG ID fjo:Fjoh_3277
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
TssN PF17555.4 3.7e-76 16..289
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MVKKHLLALIDI
Transmembrane helix 13-32 RLIVFLVVIIAVSFILTMVF
Outside 33-46 SDKIKEFAVQYKRK
Transmembrane helix 47-66 FYIYVFSFVLIYALVGFLGY
Inside 67-78 NKLFTELSDEFR
Transmembrane helix 79-98 FYQIASLLFGILNVYLYRWY
Outside 99-107 FNEFNLKGV
Transmembrane helix 108-130 VGIELLFSLLITLYSSVLFVIIY
Inside 131-136 TALNGI
Transmembrane helix 137-159 ALTFLMCSHFLVFIVPTGVYAVF
Outside 160-323 NYMMQIPPKEYVTWKIPERKNPFPEIENVEMKDLLLITLLIQKKETDTKYTSLRSKGPVRIDFGALFYHTVSGYNEHNADSKIDLKENGENCSWVFFLQPKWYQTAKYVDAKYTLSMNGITENSVIICKRQKIKKVNQTKKAEGKGEDFMFEPKVEKKEKEAAV


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9984 - -


  Protein sequence: 324 a.a.    

>T6CP011098 NC_009441:c3917662-3916691 [Flavobacterium johnsoniae UW101] [TssN]
MVKKHLLALIDIRLIVFLVVIIAVSFILTMVFSDKIKEFAVQYKRKFYIYVFSFVLIYALVGFLGYNKLFTELSDEFRFY
QIASLLFGILNVYLYRWYFNEFNLKGVVGIELLFSLLITLYSSVLFVIIYTALNGIALTFLMCSHFLVFIVPTGVYAVFN
YMMQIPPKEYVTWKIPERKNPFPEIENVEMKDLLLITLLIQKKETDTKYTSLRSKGPVRIDFGALFYHTVSGYNEHNADS
KIDLKENGENCSWVFFLQPKWYQTAKYVDAKYTLSMNGITENSVIICKRQKIKKVNQTKKAEGKGEDFMFEPKVEKKEKE
AAV*

  Nucleotide sequence: 972 bp    

>T6CP011098 NC_009441:c3917662-3916691 [Flavobacterium johnsoniae UW101] [TssN]
ATGGTAAAAAAGCATTTATTAGCCCTTATTGATATTAGGCTTATTGTATTTTTAGTAGTAATTATAGCGGTAAGTTTTAT
ACTTACCATGGTATTCAGCGATAAAATAAAAGAATTTGCGGTACAGTATAAAAGAAAGTTTTACATCTATGTTTTTTCAT
TTGTATTGATTTATGCCCTGGTTGGTTTTTTGGGCTATAATAAATTATTTACAGAGTTGTCAGACGAGTTTCGATTTTAT
CAAATTGCATCACTGCTATTCGGAATCCTTAATGTTTACTTGTATAGATGGTATTTTAATGAGTTTAATTTAAAAGGCGT
TGTAGGTATCGAATTATTGTTTTCACTTTTAATAACACTTTATTCAAGTGTATTATTTGTGATTATTTATACTGCCTTAA
ACGGGATTGCACTGACGTTTTTAATGTGTTCTCATTTCTTGGTATTTATAGTACCGACAGGCGTTTATGCAGTTTTTAAC
TATATGATGCAGATACCTCCGAAAGAATATGTTACATGGAAAATTCCGGAAAGAAAAAATCCCTTTCCTGAGATTGAAAA
CGTTGAAATGAAAGATTTATTGTTGATTACCCTATTAATTCAGAAAAAAGAAACCGATACAAAATATACTTCTTTAAGAT
CCAAAGGTCCAGTAAGAATTGATTTTGGTGCACTGTTTTATCATACGGTTTCAGGATATAATGAACATAATGCCGATAGT
AAAATTGATTTAAAAGAAAACGGCGAAAATTGCAGCTGGGTATTCTTTTTACAGCCAAAATGGTATCAAACGGCAAAATA
TGTAGATGCAAAATATACTTTGAGCATGAATGGTATTACTGAAAACTCAGTTATCATTTGTAAAAGACAAAAGATTAAAA
AAGTTAATCAAACAAAAAAAGCAGAAGGAAAAGGAGAAGATTTTATGTTTGAACCAAAGGTTGAAAAGAAGGAAAAAGAG
GCTGCAGTTTAA