SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP011049
Component protein type	TssF
T6SS ID (Type)	T6SS00907 (Type iii)
Strain	Bacteroides fragilis NCTC 9343
Replicon	chromosome
Sequence	Protein sequence (606 a.a.); Nucleotide sequence (1818 bp)
Reference	[1] Russell AB, Wexler AG, Harding BN, et al. A type VI secretion-related pathway in Bacteroidetes mediates interbacterial antagonism. Cell Host Microbe. 2014 Aug 13;16(2):227-236. doi: 10.1016/j.chom.2014.07.007. Epub 2014 Jul 25. PMID: 25070807

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BF9343_RS09420
Coordinate (Strand)	2359546..2361363 (-)
NCBI ID	499302026
RefSeq	NC_003228
Uniprot ID	UPI00004E23E9
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-605	MANLFTKESIKARMFKQAATLYDIRNIDGMDPLIRLLIEALSGEIFKLSGDMHAIESRLLEKVASALTPHTALVAKPAHAIAAARPYTPQATVSPTDLFSYKSTEIVKKYKMKNLFFTPLHETRIINAELKYLVTADEFCTITPEGERDATARFRSDVPVMGRKISIGMKIGNNVTTLNDLPLYIDIPLVADKSSYLKLLPYCHCTIAGIPVEIKGGIEYDPTRSVSEKYDLGRLITEEITSKYASHYLTLKAHGLKVRDLSRSRVPEEISFLLPSDFIAECDADTVWIDIEFPTAFSKEILEQVKVQMNTFIVVNKYPAKITRKVDSVSAILPLEKTEFEYFLFVDSITDNHGDRLREISGTQDEGRAGCYSVRRGGCERFNAMDAKDFLNRLTDLLYDESMAFSSTDKDGMKEVIEQIEERVNQLGDKNKDGVGGQEMLSYVVIDQRYDKDTRLTVDYTLTNGEFANDIYAGEPLNDCANSDIDGDTLRFVTSARGGKPSPSVKRRMDMYRFMLLSHGRIYTKEDIRNFCMARYGDSIRSVEVKLGYVAGKKESEGFIRTLDVYLRLSEGMQGLDREEFVVNLDSELRRLSPETYNYRVFINS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9967	-	-

Protein sequence: 606 a.a.

						>T6CP011049 NC_003228:c2361363-2359546 [Bacteroides fragilis NCTC 9343] [TssF]
MANLFTKESIKARMFKQAATLYDIRNIDGMDPLIRLLIEALSGEIFKLSGDMHAIESRLLEKVASALTPHTALVAKPAHA
IAAARPYTPQATVSPTDLFSYKSTEIVKKYKMKNLFFTPLHETRIINAELKYLVTADEFCTITPEGERDATARFRSDVPV
MGRKISIGMKIGNNVTTLNDLPLYIDIPLVADKSSYLKLLPYCHCTIAGIPVEIKGGIEYDPTRSVSEKYDLGRLITEEI
TSKYASHYLTLKAHGLKVRDLSRSRVPEEISFLLPSDFIAECDADTVWIDIEFPTAFSKEILEQVKVQMNTFIVVNKYPA
KITRKVDSVSAILPLEKTEFEYFLFVDSITDNHGDRLREISGTQDEGRAGCYSVRRGGCERFNAMDAKDFLNRLTDLLYD
ESMAFSSTDKDGMKEVIEQIEERVNQLGDKNKDGVGGQEMLSYVVIDQRYDKDTRLTVDYTLTNGEFANDIYAGEPLNDC
ANSDIDGDTLRFVTSARGGKPSPSVKRRMDMYRFMLLSHGRIYTKEDIRNFCMARYGDSIRSVEVKLGYVAGKKESEGFI
RTLDVYLRLSEGMQGLDREEFVVNLDSELRRLSPETYNYRVFINS*					

Nucleotide sequence: 1818 bp

						>T6CP011049 NC_003228:c2361363-2359546 [Bacteroides fragilis NCTC 9343] [TssF]
ATGGCAAATCTATTCACAAAAGAAAGCATTAAAGCCCGGATGTTCAAACAGGCGGCAACCCTATATGACATACGAAATAT
AGATGGCATGGACCCCCTTATCCGACTGCTGATTGAAGCCTTGTCGGGCGAAATTTTCAAGCTGTCCGGTGATATGCACG
CCATTGAAAGCCGATTGTTGGAGAAAGTCGCTTCCGCCCTTACTCCACACACGGCATTGGTTGCCAAACCCGCCCATGCT
ATTGCTGCTGCCCGACCATATACACCGCAAGCTACTGTATCTCCCACAGACCTGTTTTCTTATAAAAGCACGGAGATAGT
GAAGAAGTATAAGATGAAAAATCTCTTCTTTACTCCGCTGCATGAAACCCGCATAATCAATGCCGAACTGAAGTATCTCG
TGACGGCGGATGAATTTTGCACCATCACTCCCGAAGGAGAACGTGACGCCACTGCCCGCTTTCGTTCCGATGTGCCGGTA
ATGGGTCGGAAAATCAGTATCGGGATGAAAATAGGCAACAATGTAACTACTCTCAACGACTTGCCGTTATATATAGATAT
ACCGCTTGTAGCAGATAAAAGCAGTTATCTGAAATTACTGCCGTACTGCCATTGCACGATTGCCGGTATTCCTGTAGAAA
TTAAAGGGGGAATCGAGTACGACCCCACACGTTCGGTCAGTGAGAAATACGATCTTGGCAGGTTGATTACGGAAGAAATA
ACCAGCAAATATGCTTCCCATTACCTGACATTGAAAGCTCACGGATTAAAAGTCAGAGATTTGTCACGTAGCAGAGTGCC
CGAAGAAATCTCTTTCCTGCTGCCAAGTGATTTCATTGCCGAATGTGATGCGGATACTGTTTGGATAGATATAGAATTTC
CCACCGCTTTTTCAAAAGAAATACTGGAACAGGTAAAGGTGCAGATGAACACTTTTATCGTAGTAAACAAATATCCGGCA
AAGATTACCAGAAAGGTAGATTCCGTCTCGGCCATTCTCCCTTTGGAAAAGACGGAATTTGAATATTTCCTGTTTGTGGA
TTCGATTACCGATAACCACGGAGACCGGCTTAGGGAAATTTCCGGCACACAGGATGAAGGCAGAGCCGGCTGTTATTCGG
TCAGAAGGGGCGGTTGCGAACGCTTCAATGCGATGGATGCCAAAGATTTTCTGAACCGCCTGACAGACCTGCTATATGAC
GAAAGCATGGCCTTCTCTTCGACAGACAAAGACGGGATGAAAGAGGTGATCGAGCAGATAGAGGAACGGGTAAACCAATT
GGGAGATAAAAATAAAGACGGAGTCGGAGGACAGGAAATGTTGTCATATGTAGTCATTGACCAACGTTATGACAAGGATA
CCCGGTTAACTGTGGATTATACTCTTACTAATGGCGAATTTGCCAATGACATCTATGCGGGAGAGCCATTAAATGACTGC
GCCAACTCCGATATAGACGGAGATACACTGCGGTTTGTCACTTCTGCCCGTGGAGGAAAGCCGTCCCCATCCGTAAAGCG
CCGGATGGATATGTACCGATTCATGCTATTGTCACACGGCAGGATATACACGAAAGAGGATATACGCAACTTCTGCATGG
CAAGGTATGGCGACAGCATTCGCTCTGTGGAGGTAAAGCTGGGGTATGTAGCCGGTAAAAAAGAAAGCGAAGGTTTCATA
AGGACGCTGGACGTGTATCTACGGTTATCGGAGGGGATGCAAGGATTGGACAGGGAAGAGTTCGTTGTCAATTTGGACAG
TGAGCTCAGGCGGTTGTCTCCGGAGACCTATAACTACCGTGTTTTTATTAACTCATAG					